2018
DOI: 10.1016/j.aanat.2017.10.002
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Dissociation of mono- and co-culture spheroids into single cells for subsequent flow cytometric analysis

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Cited by 26 publications
(25 citation statements)
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“…This could indicate that co‐cultured spheroids proliferate and mimic better the in vivo growth situation of heterocellular tissues with increasing culture time. This was in line with former studies reporting that mono‐cultured spheroids were lower in diameter versus co‐cultured spheroids (Grasser et al, 2018). This was clearly observed when adding HUVEC to the ASC spheroids and the co‐culture spheriods increased in diameter compared with the mono‐culture spheroids, with no difference in diameter between the two co‐culture conditions (VAT‐2S).…”
Section: Discussionsupporting
confidence: 92%
“…This could indicate that co‐cultured spheroids proliferate and mimic better the in vivo growth situation of heterocellular tissues with increasing culture time. This was in line with former studies reporting that mono‐cultured spheroids were lower in diameter versus co‐cultured spheroids (Grasser et al, 2018). This was clearly observed when adding HUVEC to the ASC spheroids and the co‐culture spheriods increased in diameter compared with the mono‐culture spheroids, with no difference in diameter between the two co‐culture conditions (VAT‐2S).…”
Section: Discussionsupporting
confidence: 92%
“…The LF spheroids were first dissociated following the method reported by Grasser et al [26] Several spheroids were collected from a ULA 96-well plate in a centrifuge tube (15 mL). After precipitation of the spheroids, the supernatants were carefully removed and washed twice with 1× PBS.…”
Section: Flow Cytometrymentioning
confidence: 99%
“…Single cells within spheroids were analyzed by flow cytometry. 23 Briefly, spheroids were washed with PBS and dissociated in a solution of 0.1% (w/v) type I collagenase (Calbiochem, San Diego, California) and 0.3 U dispase (Thermo Fisher) at 37 C with gentle external agitation. After 1 hour, an equal volume of media was added to quench enzymatic activity, and cells were washed with PBS.…”
Section: Flow Cytometrymentioning
confidence: 99%