Dissociation of RecA filaments from duplex DNA by the RuvA and RuvB DNA repair proteins.

Adams, David; Tsaneva, Irina R.; West, Stephen C.

doi:10.1073/pnas.91.21.9901

Cited by 40 publications

(23 citation statements)

References 28 publications

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“…Using these experimental conditions, we could follow the interaction between RecA and dsDNA in the region of synapsis almost exclusively. Only a very small proportion of covalently closed plasmid DNA molecules was covered with RecA resulting in the formation of extremely negatively supercoiled plasmid DNA, form X, after relaxation with topoisomerase I (Ohtani et al 1982;Adams et al 1994) (Fig. 6B).…”

Section: Discussionmentioning

confidence: 99%

The duplex DNA is very underwound in the three‐stranded RecA protein‐mediated synaptic complex

Voloshin

Camerini‐Otero

1997

Genes to Cells

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Section: Discussionmentioning

confidence: 99%

The duplex DNA is very underwound in the three‐stranded RecA protein‐mediated synaptic complex

Voloshin

Camerini‐Otero

1997

Genes to Cells

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“…The action of RecG following RecA-mediated DNA strand exchange and DNA repair could move the crossover backwards and ultimately reconstruct the framework of a replication fork without the action of RuvC or a similar Holliday junction resolvase (Kuzminov 1996b). The RuvA and RuvB proteins also displace RecA protein from DNA under some in vitro conditions, suggesting an additional function for these proteins in vivo (Adams et al 1994). However, the RecFOR proteins also appear to modulate RecA filament assembly and disassembly, and the fate of RecA filaments may turn out to be a more complex affair involving interactions with the RuvAB, RecFOR, and perhaps other proteins.…”

Section: Quantifying Step 1 Under Normal Growth Conditionsmentioning

confidence: 99%

A broadening view of recombinational DNA repair in bacteria

1998

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“…RuvA and RuvB form a complex composed of one or two tetramers of RuvA and two hexamers of RuvB that catalyze branch migration of Holliday junctions (HJs) using the energy provided by ATP hydrolysis. The RuvAB helicase activity extends the heteroduplex DNA region and is thought to displace the RecA protein filament facilitating the loading of RuvC onto the HJ complex (30,31). Subsequently, the endonuclease activity of RuvC resolves the recombination intermediate by introducing nicks in two of the four DNA strands (29), producing nicked duplexes that can be sealed by a DNA ligase.…”

mentioning

confidence: 99%

Characterization of the Operon Encoding the Holliday Junction Helicase RuvAB from Mycoplasma genitalium and Its Role in mgpB and mgpC Gene Variation

Burgos

Totten

2014

J Bacteriol

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Mycoplasma genitalium is an emerging sexually transmitted pathogen associated with reproductive tract disease in men and women, and it can persist for months to years despite the development of a robust antibody response. Mechanisms that may contribute to persistence in vivo include phase and antigenic variation of the MgpB and MgpC adhesins. These processes occur by segmental recombination between discrete variable regions within mgpB and mgpC and multiple archived donor sequences termed MgPa repeats (MgPars). The molecular factors governing mgpB and mgpC variation are poorly understood and obscured by the paucity of recombination genes conserved in the M. genitalium genome. Recently, we demonstrated the requirement for RecA using a quantitative PCR (qPCR) assay developed to measure recombination between the mgpB and mgpC genes and MgPars. Here, we expand these studies by examining the roles of M. genitalium ruvA and ruvB homologs. Deletion of ruvA and ruvB impaired the ability to generate mgpB and mgpC phase and sequence variants, and these deficiencies could be complemented with wild-type copies, including the ruvA gene from Mycoplasma pneumoniae. In contrast, ruvA and ruvB deletions did not affect the sensitivity to UV irradiation, reinforcing our previous findings that the recombinational repair pathway plays a minor role in M. genitalium. Reverse transcription-PCR (RT-PCR) and primer extension analyses also revealed a complex transcriptional organization of the RuvAB system of M. genitalium, which is cotranscribed with two novel open reading frames (ORFs) (termed ORF1 and ORF2 herein) conserved only in M. pneumoniae. These findings suggest that these novel ORFs may play a role in recombination in these two closely related bacteria.W ith a genome of only 580 kb encoding 482 predicted proteins, Mycoplasma genitalium is considered the organism with the smallest known genome capable of self-replication (1). Consequently, M. genitalium has become a model organism for understanding basic biological processes (2-4) and has been used as a platform to identify a minimal gene set required for autonomous cellular life (5). M. genitalium is also a sexually transmitted human pathogen with an implicated reproductive tract disease spectrum very similar to that of Chlamydia trachomatis, including urethritis in men (6) and cervicitis, endometritis, pelvic inflammatory disease (PID), and tubal factor infertility in women (7). M. genitalium infections are often asymptomatic and persistent (8-10), possibly increasing the risk for sexual transmission and sequelae such as PID and infertility. Despite the significance of primary disease and its sequelae, the molecular pathogenesis of M. genitalium has been understudied. Barriers to such investigations include the fastidious nature and slow growth of this bacterium, difficulty of isolating contemporary strains from human specimens, and the limited genetic tools available for molecular investigations (11).In previous studies, we and others have shown that M. genitalium strains gen...

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Dissociation of RecA filaments from duplex DNA by the RuvA and RuvB DNA repair proteins.

Cited by 40 publications

References 28 publications

The duplex DNA is very underwound in the three‐stranded RecA protein‐mediated synaptic complex

The duplex DNA is very underwound in the three‐stranded RecA protein‐mediated synaptic complex

A broadening view of recombinational DNA repair in bacteria

Characterization of the Operon Encoding the Holliday Junction Helicase RuvAB from Mycoplasma genitalium and Its Role in mgpB and mgpC Gene Variation

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