Dissolution Test for Silymarin Tablets and Capsules

Campodonico, Adriana; Collado, Estela; Ricci, Rita; Pappa, Horacio; Segall, Adriana Inés; Pizzorno, M. T.

doi:10.1081/ddc-100000244

Cited by 13 publications

(4 citation statements)

References 1 publication

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“…Modifications included a methanol : water:acetic acid (35 : 60 : 5) mobile phase and UV detection at 277 nm. This preparation of silymarin was comparable to several commercial formulations characterized by Campodónico et al [11]. Mice were given five daily intraperitoneal (i.p.)…”

Section: Treatment Of Assay Groupsmentioning

confidence: 95%

“…Silymarin was purchased from Sigma-Aldrich Chemical Company (St. Louis, MO). This silymarin consists of a mixture of seven isomers including taxifolin (4 %), silichristin (27.9 %), silidianin (2.9 %), silybin A (19.3 %), silybin B (31.3 %), isosilybin A (8.2 %) and isosilybin B (2.3 %) as determined by high performance liquid chromatography as described previously [11]. Modifications included a methanol : water:acetic acid (35 : 60 : 5) mobile phase and UV detection at 277 nm.…”

Section: Treatment Of Assay Groupsmentioning

confidence: 99%

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Physiological Responses to a Natural Antioxidant Flavonoid Mixture, Silymarin, in BALB/c Mice: II. Alterations in Thymic Differentiation Correlate with Changes in c-mycGene Expression

Johnson¹,

Osuchowski²,

He³

et al. 2002

Planta med

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Silymarin is a mixture of bioactive flavonoids isolated from the seeds and fruits of Milk Thistle [ Silybum marianum (L.) Gaertner]. We tested the hypothesis that exposure to silymarin will modulate differentiation and cell selection in the thymus via alterations in gene expression. Male BALB/c mice were treated intraperitoneally once daily for five days with 0, 10, 50 or 250 mg/kg of silymarin. Flow cytometric examination of thymic lymphocyte populations showed that the absolute numbers of CD4 + and CD8 + positive T-lymphocytes were increased by silymarin. The c- myc proto-oncogene is important in controlling differentiation and functions of thymocytes. Treatment with silymarin resulted in increased c- myc expression in the thymus. In contrast, the expressions of IL-2 and IL-4 were decreased by silymarin, while MHC II expression did not change. These results indicate that in vivo exposure to silymarin influences phenotypic selection processes in the thymus at doses that may be encountered in natural medicinal use. Further studies investigating the effects of silymarin on the immune system are warranted.

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Section: Treatment Of Assay Groupsmentioning

confidence: 95%

Section: Treatment Of Assay Groupsmentioning

confidence: 99%

Physiological Responses to a Natural Antioxidant Flavonoid Mixture, Silymarin, in BALB/c Mice: II. Alterations in Thymic Differentiation Correlate with Changes in c-mycGene Expression

Johnson¹,

Osuchowski²,

He³

et al. 2002

Planta med

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“…The stationary phase, Bondclone C 18 column (300 mm x 3.9 mm, 10 µm -Phenomenex, CA, USA), was kept at 25°C. The mobile phase was a mixture of methanol : double distilled water : acetic acid (50:50:1) 21 . The flow rate was 1.0 ml/min and the run time was 30 min.…”

Section: High Performance Liquid Chromatography (Hplc) Of Sesmentioning

confidence: 99%

Preparation, Characterization and Evaluation of Fast-Dissolving Silibininenriched Silymarin (Ses)

Khaled

2008

Bulletin of Pharmaceutical Sciences. Assiut

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The hepato-protective activity of silymarin is well demonstrated. However, silymarin is not a single component, but a mixture of silydianin, silychristin and silibinin. Silibinin is the least soluble and the most active component of silymarin. Accordingly, as silibinin content of silymarin increases, its activity is expected to increase. These were the objectives of the study i.e to prepare silibinin enriched silymarin (SES) and enhance its dissolution by preparing its adsorbates and co-adsorbates with Florite. Silibinin enriched silymarin was prepared by extracting the water soluble components of silymarin using water. The silibinin content of the SES was evaluated using HPLC method of assay. The fast dissolving SES systems were characterized using differential scanning calormetry, x-ray diffractometry and infra-red spectroscopy. The obtained results indicated the compatibility of Florite with SES. The biological activity of SES systems was evaluated in rats using paracetamol as a hepatotoxic agent and compared to that of silibinin alone. The results showed that SESco-adsorbate was more efficient in lowering the serum level of the specific liver enzymes (ALT and AST) than silibinin alone.

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“…Silybin is one of the major flavonolignans from the mixture & consists of about 60-70% of silymarin. Silybin is a mixture of two diastereomers (A, B) which are present in a ratio of 1:1 [1,2]. Silybin is a known hepatoprotectant but due to its low serum, tissue levels, low solubility & absorption lead to its poor oral bioavailability [3,4].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Biological Evaluation of Silybin Liposomes for the Treatment of Liver Disorders

Gandey¹,

Aparna

Kandarapu³

2021

JPRI

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Aim: The aim of the present study was to develop silybin liposome by incorporating phosphatidyl choline & cholesterol so as to increase its oral bioavailability and liver targeted enhanced hepatoprotection. Methodology: Thin film hydration technique was used for the development of liposomes by using phosphatidyl choline, cholesterol and drug. Liposomes were evaluated for vesicle size, zeta potential, PDI, encapsulation efficiency, surface morphology and in vitro drug release study. Further the optimized formulation was evaluated for APAP-induced alterations in liver and kidney function tests in rats and histopathological studies. Results: The results were promising with a sustained drug release of 80% within 20hrs, optimized vesicle size of 276nm and 89% encapsulation efficiency. The animal studies demonstrated superior hepatoprotective effect compared to silybin solution. Conclusion: The silybin liposomes showed better in-vitro release & in-vivo hepatoprotection along with better animal activity & improvement in histopathological changes as compared to silybin.

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Dissolution Test for Silymarin Tablets and Capsules

Cited by 13 publications

References 1 publication

Physiological Responses to a Natural Antioxidant Flavonoid Mixture, Silymarin, in BALB/c Mice: II. Alterations in Thymic Differentiation Correlate with Changes in c-mycGene Expression

Physiological Responses to a Natural Antioxidant Flavonoid Mixture, Silymarin, in BALB/c Mice: II. Alterations in Thymic Differentiation Correlate with Changes in c-mycGene Expression

Preparation, Characterization and Evaluation of Fast-Dissolving Silibininenriched Silymarin (Ses)

Preparation and Biological Evaluation of Silybin Liposomes for the Treatment of Liver Disorders

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