Dissolved air flotation as potential new mechanism for intestinal parasite diagnosis in feces

Soares, Felipe Augusto; Santos, Bianca Martins dos; Rosa, Stefany Laryssa; Loiola, Saulo Hudson Nery; Suzuki, Celso Tetsuo Nagase; Sabadini, Edvaldo; Bresciani, Kátia Denise Saraiva; Falcão, Alexandre X.; Gomes, Jancarlo Ferreira

doi:10.1016/j.actatropica.2021.106137

Cited by 4 publications

(4 citation statements)

References 37 publications

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“…oocysts from wastewater in several studies focused on the physical-chemical treatment phase for water reuse [13,14]. The technical viability of this principle for the detection of four parasitic species in faecal suspension was confirmed in a study that presented an interaction of microbubbles with the surface of eggs of Ascaris lumbricoides and Hymenolepis diminuta, larvae of Strongyloides stercoralis, and cysts of G. duodenalis, with the positive recovery of parasites contained in faecal samples [15].…”

Section: Introductionmentioning

confidence: 90%

“…The protocol described by Soares et al [15] was used at each stage of the laboratory evaluation of DAF parameters. The following initial parameters were adopted for air saturation and saturated liquid volume proportion inserted in the system: pH 7-7.2; temperature 20 -26 C; pressure 5 bar; saturation time 15 min; and saturated liquid proportion 10%.…”

Section: Standardisation Testsmentioning

confidence: 99%

“…For the total count of helminth eggs and larvae, a 500 μl aliquot was transferred to 10 microscope slides (26 by 76 mm) (50 μl per slide) for complete scanning of the coverslip area (24 by 24 mm) with a microscope. For an estimated protozoan count, we prepared 10 slides and obtained five micrographs (20Â magnification) from the central areas of the slide to record the total number of cysts in 50 images of each sample (floated or non-floated) [15,16]. We evaluated the DAF parameters adopted in the experiments regarding the number of parasites counted in the floated and nonfloated materials according to the flowchart in Figure S1.…”

Section: Standardisation Testsmentioning

confidence: 99%

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Detection of intestinal parasites in human faecal samples using dissolved air flotation

Soares

Santos

Rosa

et al. 2022

Tropical Med Int Health

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Objective Ova and parasite (O&P) examination is recommended for the laboratory diagnosis of agents causing parasitic infections; however, this exam requires scientific and technological improvements to enhance its diagnostic validity. Dissolved air flotation (DAF) is an efficient technical principle separating suspended solids in a liquid medium. We aimed to develop and validate a new procedure for intestinal parasite detection with DAF. Methods In this study, we collected samples from 500 volunteers, screened them by direct examination, and transferred the material to tubes using the Three Faecal Test (TF‐Test) for triplicate DAF tests. We evaluated physical–chemical parameters and DAF prototype components through quantifying parasites recovered from floated and non‐floated regions of the flotation column. The DAF operation protocol was validated with the gold standard results. Results The 10% saturated volume proportion and cationic surfactant showed regularity and high parasite recovery (80%). Modifications of the needle device did not influence parasite recovery (p > 0.05). Sensitivity, specificity, accuracy and kappa agreement obtained with the DAF protocol were 91%, 100%, 93% and substantial (k = 0.64), respectively. Conclusion The DAF principle could be used to process faecal samples in routine laboratory exams, enabling intestinal parasite detection.

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Section: Introductionmentioning

confidence: 90%

Section: Standardisation Testsmentioning

confidence: 99%

Section: Standardisation Testsmentioning

confidence: 99%

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Detection of intestinal parasites in human faecal samples using dissolved air flotation

Soares

Santos

Rosa

et al. 2022

Tropical Med Int Health

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“…The results showed parasite recovery efficiencies of 73.27 %, 58.12 %, 37.85 %, and 91.89 % for Ascaris lumbricoides, Hymenolepis diminuta, Giardia duodenalis, and Strongyloides stercoralis, respectively. This reveals a promising line of research for DAF, as intestinal parasitosis is a severe public health problem globally [40].…”

Section: Introductionmentioning

confidence: 96%

Dissolved Air Flotation: A Review from the Perspective of System Parameters and Uses in Wastewater Treatment

Muñoz-Alegría

España

Flórez-Marulanda

2021

TecnoL.

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The current issues of climate change and high freshwater demand worldwide have promoted the implementation of wastewater reclamation technologies. This study aims to review the efficiency of the dissolved air flotation (DAF) technique in a wide variety of applications in the agricultural, industrial, domestic, and municipal sectors, which have high freshwater consumption worldwide. We made a systematic review of the DAF technique in wastewater treatment in 2015-2021. We reviewed six indexed databases and governmental statistical reports; we used the keywords: dissolved air flotation, microbubbles, wastewater treatment, and the main operating and design parameters involved in the effectiveness of the flotation process. Additionally, we conducted a review of the most common synthetic coagulant studies used with DAF, as well as natural coagulants that promise to mitigate current climate change. Finally, we discussed advantages, disadvantages, and potential future studies. DAF to have considerable potential for wastewater treatment, as well as for waste utilization. The generation of large quantities of DAF sludge is a breakthrough for clean energy production, as it allows the use of this waste for biogas production.

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An in-depth analysis of the hydrodynamic behaviour of a high-rate lamellar DAF tank by means of CFD simulations

Hlukhov,

Larraona,

Rivas

et al. 2024

Appl Water Sci

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This research is based on computational fluid dynamics simulations of water and microbubble flow within the tank of a lamellar DAF (L-DAF) clarification system operating under high-rate DAF conditions (12–30 m/h). Firstly, performance of the DAF tank with lamellae was evaluated under two operating conditions in which the flow was either short-circuited or stratified in the absence of lamellae. In addition, the improvement in bubble removal efficiency achieved by the incorporation of lamellae in each scenario was assessed. Secondly, an in-depth analysis was conducted of the flow that develops in the separation zone as a result of placing the lamella pack in that part of the tank. The significant density difference that the lamellae cause to exist between the bubble blanket and clarified water below is responsible for the complex three-dimensional flow observed between the two regions. Analysis of this flow showed a previously undescribed mechanism in which the density gradient plays a crucial role in preventing bubbles from passing through the lamellae and ultimately escaping with the effluent. Finally, the effect of hydraulic loading on the bubble removal efficiency of the L-DAF tank under consideration was researched, and it was found that an L-DAF with a height/length ratio of 0.72 is able to operate at hydraulic loading close to 30 m/h, evidencing good debubbling performance.

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Dissolved air flotation as potential new mechanism for intestinal parasite diagnosis in feces

Cited by 4 publications

References 37 publications

Detection of intestinal parasites in human faecal samples using dissolved air flotation

Detection of intestinal parasites in human faecal samples using dissolved air flotation

Dissolved Air Flotation: A Review from the Perspective of System Parameters and Uses in Wastewater Treatment

An in-depth analysis of the hydrodynamic behaviour of a high-rate lamellar DAF tank by means of CFD simulations

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