Kolleru Lake is the largest natural freshwater lake in India. Sediment samples were collected at different locations from Kolleru Lake at different seasons in a year over a period of three years and analyzed for eight selected pesticides. In general, pesticides were present at all locations. The selected pesticides alpha-BHC, gamma-BHC, malathion, chlorpyrifos and endosulfan were present at higher concentrations than isodrin, dieldrin and p,p'-DDT. The concentrations of alpha-BHC, gamma-BHC malathion, chlorpyrifos, isodrin, endosulfan, dieldrin and p,p'-DDT in sediments were 1.2-388, 1.4-212, from below the minimum detection limit (BDL)-186, 1.1-292, BDL-38.6, BDL-206, BDL-128 and BDL-191 micrograms kg-1 dry weight, respectively. The aim of this study is to evaluate baseline data, to try to account for the concentration of pesticides in sediments and to discuss their potential to affect human beings and aquatic organisms.