Dissolved organic substrates as phosphorus and nitrogen sources for axenic batch cultures of freshwater green algae

Berman, T.; Chava, S.; Kaplan, Bina; Wynne, David

doi:10.2216/i0031-8884-30-4-339.1

Cited by 26 publications

(19 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While the quantitative role of DON as a nitrogen source for autotrophs is debated (Bronk et al, 2007), potential mechanisms of utilization, such as bacterial ammonification and subsequent phytoplankton uptake of the released nitrogen (Antia et al, 1991;Berman et al, 1991), are indicated for many estuarine settings. In a microcosm study on turnover of low-(LMW-DON) and high-molecular weight DON (HMW-DON) in waters from the Elbe estuary, LMW-DON was preferentially removed and nitrified over HMW-DON (Kerner and Spitzy, 2001).…”

Section: Don Turnover In the Elbe Estuarymentioning

confidence: 99%

Turnover of combined dissolved organic nitrogen and ammonium in the Elbe estuary/NW Europe: Results of nitrogen isotope investigations

Schlarbaum¹,

Dähnke²,

Emeis³

2010

Marine Chemistry

View full text Add to dashboard Cite

Section: Don Turnover In the Elbe Estuarymentioning

confidence: 99%

Turnover of combined dissolved organic nitrogen and ammonium in the Elbe estuary/NW Europe: Results of nitrogen isotope investigations

Schlarbaum¹,

Dähnke²,

Emeis³

2010

Marine Chemistry

View full text Add to dashboard Cite

“…'Mix A was 0.25 pM P each of F-1,6-P, G-1-P, GYP and PEP 'Mix B was 0.25 pM P each of ATP, GDP, UDP and PPP rus may ultimately control biomass and organic production in certain marine environments [Smith et al 1986, teria and phytoplankton do have enzyme systems appropriate for DOP hydrolysis (Chrost & Overbeck 1987, Tarapchak & Moll 1990). Consequently, they are able existing Organic phosphorus sources, though to varying degrees (Chu 1946, Kuentzler 1965, 1970, Currie 1986, Bentzen & Taylor 1991, Berman et al 1991, Codispoti 1989. In oligotrophic oceanic habitats, such as the central North Pacific Ocean (Karl et al 1993), the regeneration of phosphate within the euphotic zone could be an important regulating factor.…”

Section: Size Fractionation Experimentsmentioning

confidence: 99%

Bioavailability of inorganic and organic phosphorus compounds to natural assemblages of microorganisms m Hawaiian coastal waters

Björkman¹,

Karl²

1994

Mar. Ecol. Prog. Ser.

176

129

View full text Add to dashboard Cite

The bioavailability of 7 organic and 2 inorganic phosphorus compounds to natural communities of coastal marine bacteria and phytoplankton was evaluated. A bioavailability factor (BF) based on changes in the turnover time of the phosphate pool (measured using 3'P0,) in the absence and presence of selected phosphorus compounds, relative to positive controls receiving PO,, was calculated as an index of the relative microbial metabolic preference for each added compound. There were marked differences in the bioavailabihty factors of various substrates tested with values ranging from 0 to 0.2. The results indicate that nucleotides were the most readily utilizable of the combined phosphorus compounds investigated. By comparison, the addition of selected monophosphate esters, either individually or in mixtures, had only a lirmted effect on orthophosphate (PO,) flux. However, orthophosphate appeared to be the preferred and, apparently, universal substrate. The bacterialennched slze fraction (< 0 8 pm) comprised an average of 75 % of the total phosphorus uptake measured in our samples A large but variable percentage of the added organic and combined inorganic compounds accunlulated outside the cells as soluble reactive phosphorus (SRP), indicating an efficient regeneration of orthophosphate from the various phosphorus compounds tested

show abstract

“…Some DON compounds can be taken up directly by bacteria (Flynn & Butler 1986, Paul et al 1986, Sirnon & Rosenstock 1992 or by phytoplankton (Antia et al 1975, Kapp et al 1975, Neilson & Larson 1980, Oliveira & Huynh 1989, Berman et al 1991. Field research also proved that urea is an important potential nitrogen source for phytoplankton in several coastal waters (McCarthy 1972, Price & Harrison 1988, Tamminen & Irmisch 1996, and dissolved free amino acids have been reported to sustain a significant part of algal growth in the Chesapeake Bay (Glibert et al 1991).…”

Section: Introductionmentioning

confidence: 97%

Release of ammonium and urea from dissolved organic nitrogen in aquatic ecosystems

Berman¹,

Béchemin²,

Maestrini³

1999

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

The potential for release of ammonium (NH,+) a n d o r urea from the pool of dissolved organic nitrogen (DON) was examined in san~ples taken from Lake l n n e r e t (Israel), the k v e r Charente estuary and coastal water near Ile de Re (French Atlantic coast). After prefiltration through 1.0 or 1.2 pm membranes to remove most of the microbiota with the exception of bacteria, water samples with or without supplements (40 pM) of various organic nitrogen compounds (arginine, glucosamine, guanine, hypoxanthine, lysine, ornithine or thymine) were incubated at in situ temperatures, in the dark. for 7 to 14 d. Concentrations of NH,' and urea were monitored during the incubation period. Increases of NH,' with time were observed in 8 out of 12 experiments with unsupplemented lake samples, and in a single trial with coastal water, but not with Charente estuary water. In some expenments, increases of urea concentrations were also observed. The addition of organic nitrogen compounds almost always led to NH4+ increases in samples from all locations; guanine, hypoxanthine, arginine and, in the case of Charente water, glucosamine gave rise to urea. The addition of nitrification inhibitors (40 pM) at the start of some experiments gave inconsistent results, but in some cases appeared to increase the concentrations of NH,' with time. Taken together, the results of these experiments clearly indicate the potential in natural waters for degradation of DON pool constituents by indigenous bactena a n d o r free dssolved enzymes to NH,+ or urea; these in turn can be effectively exploited by the ambient microbiota. The breakdown of DON with the concomitant release of readily available compounds such as NH,' or urea could be an important process in the nitrogen nutrition of phytoplankton and bacteria.

show abstract

Dissolved organic substrates as phosphorus and nitrogen sources for axenic batch cultures of freshwater green algae

Cited by 26 publications

References 0 publications

Turnover of combined dissolved organic nitrogen and ammonium in the Elbe estuary/NW Europe: Results of nitrogen isotope investigations

Turnover of combined dissolved organic nitrogen and ammonium in the Elbe estuary/NW Europe: Results of nitrogen isotope investigations

Bioavailability of inorganic and organic phosphorus compounds to natural assemblages of microorganisms m Hawaiian coastal waters

Release of ammonium and urea from dissolved organic nitrogen in aquatic ecosystems

Contact Info

Product

Resources

About