A graphical method for determining the 5-day biochemical
oxygen
demand (BOD5) for a body of water is described. In this
bioassay, students collect a sample of water from a designated site,
transport it to the laboratory, and evaluate the amount of oxygen
consumed by naturally occurring bacteria during a 5-day incubation
period. An accuracy check, prepared by spiking a portion of the water
with a glucose–glutamic acid solution, is used to evaluate
the student’s technique. This graphical treatment requires
a larger number of samples than the standard method described in the
literature, but it is not prone to errors caused by the quality of
dilution water, which can invalidate measurements made with the standard
method.