Dissolving Classroom by Melissa Tanaka

Engineered Biosynthesis of Fungal 4-Quinolone Natural Products

“…Three candidate amidases were found in the A. nidulans genome, including GmdS (accession number, AAL10515), which was shown by whole-cell assays to hydrolyze benzamide and phenylacetamide. 18 We cloned and expressed GmdS and assayed its activity in the presence of 4. The complete consumption of 4 and the rapid formation of 6 were observed in the presence of GmdS, which is consistent with the hydrolysis to an amide, followed by a much more facile intramolecular lactam formation (Figure S15).…”