The study goal is to solve the problem of the diagnosis of triceps crus injury of volleyball players, meet the needs of volleyball players and team doctors for the correct diagnosis of triceps crus injury scanning, make up for the deficiency that triceps crus injury scanning diagnosis is easy to make mistakes, and improve the efficiency and ability of triceps crus injury diagnosis scanning. Because the experiment involves the technical action of volleyball jump serve, DELSYSR Trignomobile wireless portable surface electromyography tester (16 leads) made in the United States is selected to test the surface electromyography of the main muscle groups of college male volleyball players in the process of jump serve. The German made simi-3D motion image system is used to conduct three-dimensional synchronous test of athletes’ jump serve action. The data analysis software adopts EMG work analysis, EMG analysis software, and simi-3D motion image analysis system for postprocessing data. The original signal is filtered (400 Hz for low pass and 20 Hz for high pass) and rectified. Finally, IEMG, EMG contribution rate, and RMS were calculated. This ensures the accuracy of the experiment.