Distance-dependent visual fluorescence immunoassay on CdTe quantum dot–impregnated paper through silver ion-exchange reaction

Huang, Lingting; Wang, Jun; Wang, Qingshui; Tang, Dianping; Lin, Yao

doi:10.1007/s00604-020-04546-7

Cited by 26 publications

(18 citation statements)

References 31 publications

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“…However, different from Hg 2+ , the emission spectra of CdTe QDs quenched by Ag + ions exhibit an obvious red shift accompanied by the changes in the spectra profiles. As discussed in the former reports, the mechanism for the CdTe QD fluorescence quenching was that the binding of Ag + with bare Te atoms could form a Ag 2 Te species on the surface of the QDs and thus alter the surface state of QDs [ 35 ]. As for Hg 2+ , the quenching mechanism is complex: electron transfer as well as ion-binding can result in the reduction of QD emission intensity.…”

Section: Resultsmentioning

confidence: 99%

Dual-Emission Fluorescence Probe Based on CdTe Quantum Dots and Rhodamine B for Visual Detection of Mercury and Its Logic Gate Behavior

Gao

Liu

et al. 2021

Micromachines

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It is urgent that a convenient and sensitive technique of detecting Hg2+ be developed because of its toxicity. Conventional fluorescence analysis works with a single fluorescence probe, and it often suffers from signal fluctuations which are influenced by external factors. In this research, a novel dual-emission probe assembled through utilizing CdTe quantum dots (QDs) and rhodamine B was designed to detect Hg2+ visually. Only the emission of CdTe QDs was quenched after adding Hg2+ in the dual-emission probe, which caused an intensity ratio change of the two different emission wavelengths and hence facilitated the visual detection of Hg2+. Compared to single emission QDs-based probe, a better linear relationship was shown between the variation of fluorescence intensity and the concentration of Hg2+, and the limit of detection (LOD) was found to be11.4 nM in the range of 0–2.6 μM. Interestingly, the intensity of the probe containing Hg2+ could be recovered in presence of glutathione (GSH) due to the stronger binding affinity of Hg2+ towards GSH than that towards CdTe QDs. Based on this phenomenon, an IMPLICATION logic gate using Hg2+/GSH as inputs and the fluorescence signal of QDs as an output was constructed.

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Section: Resultsmentioning

confidence: 99%

Dual-Emission Fluorescence Probe Based on CdTe Quantum Dots and Rhodamine B for Visual Detection of Mercury and Its Logic Gate Behavior

Gao

Liu

et al. 2021

Micromachines

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“…There are also other types of visible fluorescence immunoassay impregnated on paper that may have great potential for heparin detection. Huang et al [ 70 ] developed a paper-based analytical device (PAD) that Ag + fluorescently quenched CdTe quantum dots (QD) on nitrocellulose films by distance dependent silver ion exchange reaction, followed by Sandwich immune reactions between silver nanoparticles labelled secondary antibodies and the microtitration pores coated with primary antibodies to test human serum samples. Meanwhile, Lv et al [ 71 ] produced wet NH 3 by sandwich immunoassay using highly functionalized gold nanoparticles with glutamate dehydrogenase and secondary antibody.…”

Section: Analysis Of Heparinsmentioning

confidence: 99%

Techniques for Detection of Clinical Used Heparins

Zhao

2021

International Journal of Analytical Chemistry

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Heparins and sulfated polysaccharides have been recognized as effective clinical anticoagulants for several decades. Heparins exhibit heterogeneity depending on the sources. Meanwhile, the adverse effect in the clinical uses and the adulteration of oversulfated chondroitin sulfate (OSCS) in heparins develop additional attention to analyze the purity of heparins. This review starts with the description of the classification, anticoagulant mechanism, clinical application of heparins and focuses on the existing methods of heparin analysis and detection including traditional detection methods, as well as new methods using fluorescence or gold nanomaterials as probes. The in-depth understanding of these techniques for the analysis of heparins will lay a foundation for the further development of novel methods for the detection of heparins.

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“…Moreover, multiple detection channels also can be made on a piece of paper to achieve simultaneous detection of multiple targets [8][9][10][11]. The μPAD detection principle was mainly based on the change of color and distance [12][13][14]. For the ones based on color change, it was difficult to achieve high visual resolution and high sensitivity because it was difficult to judge unobvious changes in color with naked eyes.…”

Section: Introductionmentioning

confidence: 99%

Distance-Based Detection of Ag+ with Gold Nanoparticles-Coated Microfluidic Paper

Cui²,

Wen

et al. 2021

J. Anal. Test.

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In this study, we developed a microfluidic paper analysis device (μPAD) for distance-based detection of Ag + in water. The μPAD was manufactured by wax printing method on filter paper. Then, a layer of gold nanoparticles (AuNPs) was deposited and ascorbic acid was printed on the channel. In the detection, Ag + was reduced by ascorbic acid and coated on the surface of the AuNPs on the channel, forming Au@Ag core/shell nanoparticles. Based on the capillary flow principle, different concentrations of Ag + formed different distances of color ribbons. Thus, quantitative detection of Ag + can be achieved by measuring the distance of the color ribbon. The detection limit of this method was as low as 1 mg•L −1 within 15 min and the interference of common metal ions in water can be eliminated. In conclusion, this method had successfully realized the leap from colorimetry to direct reading, realizing fast read and easy manipulation with low-cost.

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Distance-dependent visual fluorescence immunoassay on CdTe quantum dot–impregnated paper through silver ion-exchange reaction

Cited by 26 publications

References 31 publications

Dual-Emission Fluorescence Probe Based on CdTe Quantum Dots and Rhodamine B for Visual Detection of Mercury and Its Logic Gate Behavior

Dual-Emission Fluorescence Probe Based on CdTe Quantum Dots and Rhodamine B for Visual Detection of Mercury and Its Logic Gate Behavior

Techniques for Detection of Clinical Used Heparins

Distance-Based Detection of Ag+ with Gold Nanoparticles-Coated Microfluidic Paper

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