We provide an open-source user-friendly graphical-user interface software in a MATLAB environment, named Speckle Analyzer, as a tool for calculating and analyzing statistical parameters of a laser speckle pattern to find metrics for an object’s physical quantity. The first- and second-order statistical functions containing gray-level co-occurrence and gray-level run-length matrices and speckle grains geometrical properties are included in Speckle Analyzer. To validate the software’s operation, statistical parameters of the laser speckle pattern, to find metrics for the size and concentration of particles suspended in liquid, are investigated.