Distilling Consciousness: Isolated the Underlying Brain Activity?

Macerollo, Antonella; Quattrocchi, Graziella

doi:10.1523/jneurosci.2020-14.2014

Cited by 1 publication

(1 citation statement)

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“…For all samples, cortical tissue was utilized in order to gain a better perspective in regions associated with loss of righting reflex/drug-induced loss of consciousness [19, 20]. Phosphatase inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA) was added to tissue collected for phosphorylation analysis.…”

Section: Methodsmentioning

confidence: 99%

Cytoplasmic Phospholipase A2 Modulation of Adolescent Rat Ethanol-Induced Protein Kinase C Translocation and Behavior

et al. 2015

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Ethanol consumption typically begins during adolescence, a developmental period which exhibits many age-dependent differences in ethanol behavioral sensitivity. Protein kinase C (PKC) activity is largely implicated in ethanol-behaviors, and our previous work indicates that regulation of novel PKC isoforms likely contributes to decreased high-dose ethanol sensitivity during adolescence. The cytoplasmic Phospholipase A2 (cPLA2) signaling cascade selectivity modulates novel and atypical PKC isoform activity, as well as adolescent ethanol hypnotic sensitivity. Therefore, the current study was designed to ascertain adolescent cPLA2 activity both basally and in response to ethanol, as well as it's involvement in ethanol-induced PKC isoform translocation patterns. cPLA2 expression was elevated during adolescence, and activity was increased only in adolescents following high-dose ethanol administration. Novel, but not atypical PKC isoforms translocate to cytosolic regions following high-dose ethanol administration. Inhibiting cPLA2 with AACOCF3 blocked ethanol-induced PKC cytosolic translocation. Finally, inhibition of novel, but not atypical, PKC isoforms when cPLA2 activity was elevated, modulated adolescent high-dose ethanol-sensitivity. These data suggest that the cPLA2/PKC pathway contributes to the acute behavioral effects of ethanol during adolescence.

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Section: Methodsmentioning

confidence: 99%