Distinct Anabolic Response of Osteoblast to Low-Intensity Pulsed Ultrasound

Naruse, Keiko; Miyauchi, Akimitsu; Itoman, Moritoshi; Mikuni-Takagaki, Yuko

doi:10.1359/jbmr.2003.18.2.360

Cited by 152 publications

(106 citation statements)

References 72 publications

(137 reference statements)

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“…In line with this finding, Naruse et al 35 demonstrated that PI3K inhibitors blunted an anabolic response to pulsed ultrasound treatments in murine, bonederived marrow cells. Although PI3K is activated mostly by receptor tyrosine kinases (RTKs), PI3K activation by some G-protein coupled receptors (GPCRs) has also been reported.…”

Section: Discussionmentioning

confidence: 74%

Pulsed electromagnetic fields rapidly modulate intracellular signaling events in osteoblastic cells: Comparison to parathyroid hormone and insulin

Schnoke

Midura

2007

Journal Orthopaedic Research

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Pulsed electromagnetic field (PEMF) devices are approved for the healing of bone nonunions, but there is a lack of understanding as to their mechanism of action at the cell and molecular level. Intermittent parathyroid hormone (PTH) therapy is currently utilized for treatment of osteoporosis, and is also being investigated for the purpose of augmenting fracture healing. Insulin and IGF-1 are also thought to play important anabolic roles in osteogenesis. In this report, signaling pathways activated by acute PTH or insulin treatments were compared to those activated by PEMF treatment in osteoblast-like cells. Some signaling molecules like the extracellular response kinases 1/2 (Erk1/2) and the cAMP response element binding protein (CREB) were activated by insulin and PTH, respectively, but not by PEMF treatment. Other signaling molecules like the insulin receptor substrate-1 (IRS-1), the S6 ribosomal subunit kinase, and the endothelial nitric oxide synthase (eNOS) were phosphorylated by PTH, insulin, and PEMF to the same relative extent and within the same time frame. IRS-1, eNOS, and S6 have been implicated in bone anabolism, and our results suggest that the anabolic effects of PEMF may be mediated, in part, through the activation of these proteins. ß

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Section: Discussionmentioning

confidence: 74%

Pulsed electromagnetic fields rapidly modulate intracellular signaling events in osteoblastic cells: Comparison to parathyroid hormone and insulin

Schnoke

Midura

2007

Journal Orthopaedic Research

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“…Moreover, high cellularity and less mineralization at the early stage of consolidation might be in favor of LIPUS treatment. Periosteal cells, 32 marrow stem cells, 33 and less mature osteoblasts, 34 which were found in distraction callus, were stimulated by LIPUS in vitro studies.…”

Section: Discussionmentioning

confidence: 95%

Low intensity pulsed ultrasound accelerated bone remodeling during consolidation stage of distraction osteogenesis

Chan

Qin

Lee

et al. 2005

Journal Orthopaedic Research

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Bone regeneration in distraction osteogenesis occurs under tensile stress with axial rhythmic distraction after osteotomy. In this study, we evaluated if the low intensity pulsed ultrasound (LIPUS) was also effective on enhancement of bone remodeling during consolidation stage of distraction osteogenesis. Open osteotomy of seventeen 18-week-old female New Zealand rabbit tibiae were performed. The distraction was applied with the rate of 1 mm per day. LIPUS (30 mW/cm 2 , 1.5 MHz) was delivered for 20 min per day during 4-week consolidation stage (n ¼ 10). The animals without treatment served as sham group (n ¼ 7). Plain X-ray, peripheral quantitative computational tomography (pQCT), and torsional test were performed. Results showed that smaller radiolucent interzone of LIPUS treatment group was gradually occupied by calcified tissue in plain X-ray at week 2. The bone mineral density (BMD) measured on radiographs increased by 9.18% in the LIPUS group. Bone mineral content (BMC), hard callus volume, and bone strength index (BSI) measured by pQCT were 83%, 116%, and 94%, respectively, in LIPUS group that were significantly greater than those of the controls. At the 4th week, LIPUS-treated callus showed the development of neocorticalization in the proximal and distal region. The BMC, hard callus volume, and BSI of LIPUS group decreased and was not significantly different from control. This was also confirmed by the maximum torque of LIPUS-treated callus (1424.2 AE 457.3 N Á mm) obtained at week 4, which did not differ from that of the sham group (1968.8 AE 895.1 N Á mm). In conclusion, the effective period of LIPUS treatment was at the initial stage of consolidation, with accelerated bone formation and remodeling. ß

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“…Low intensity pulsed ultrasound (LIPUS) is mechanical stress that is already used as a clinical application to promote the healing of bone fracture (9). Several in vitro studies have shown that LIPUS facilitates osteoblast differentiation, represented by increased Osteocalcin mRNA expression and extracellular calcification (10). We have reported previously that mRNA expression of several chemokines in mature osteoblasts that is mediated by angiotensin 2 type 1 receptor is also induced with LIPUS treatment (11).…”

Section: Multipotent Mesenchymal Stem Cells (Mscs)mentioning

confidence: 99%

“…LIPUS Stimulation Suppressed Adipogenic Differentiation and Promoted Osteogenic Differentiation-Previous studies have reported that osteogenic differentiation of osteoblasts is significantly promoted by daily LIPUS stimulation (10). However, the influence of LIPUS on adipogenic differentiation has never been elucidated.…”

Section: Lipus Induced Erk Phosphorylation In Preadipocytesmentioning

confidence: 99%

Low Intensity Pulsed Ultrasound (LIPUS) Influences the Multilineage Differentiation of Mesenchymal Stem and Progenitor Cell Lines through ROCK-Cot/Tpl2-MEK-ERK Signaling Pathway

Kusuyama

Bandow

Shamoto

et al. 2014

Journal of Biological Chemistry

125

107

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Background: Low intensity pulsed ultrasound (LIPUS) is a mechanical stimulus clinically used to promote bone fracture healing. Results: LIPUS suppresses adipogenesis and promotes osteogenesis of mesenchyme stem/progenitor cell lines by inhibiting PPAR␥2 through ROCK-Cot/Tpl2-MEK-ERK pathway. Conclusion: LIPUS influences multilineage differentiation of mesenchymal stem and progenitor cells. Significance: LIPUS may be a new clinical approach to chronic bone metabolic disorders, including osteoporosis.

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Distinct Anabolic Response of Osteoblast to Low-Intensity Pulsed Ultrasound

Cited by 152 publications

References 72 publications

Pulsed electromagnetic fields rapidly modulate intracellular signaling events in osteoblastic cells: Comparison to parathyroid hormone and insulin

Pulsed electromagnetic fields rapidly modulate intracellular signaling events in osteoblastic cells: Comparison to parathyroid hormone and insulin

Low intensity pulsed ultrasound accelerated bone remodeling during consolidation stage of distraction osteogenesis

Low Intensity Pulsed Ultrasound (LIPUS) Influences the Multilineage Differentiation of Mesenchymal Stem and Progenitor Cell Lines through ROCK-Cot/Tpl2-MEK-ERK Signaling Pathway

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