Distinct basolateral amygdala excitatory inputs mediate the somatosensory and aversive-affective components of pain

Combined Experiments with <i>in Vivo</i> Fiber Photometry and Behavior Tests Can Facilitate the Measurement of Neuronal Activity in the Primary Somatosensory Cortex and Hyperalgesia in an Inflammatory Pain Mice Model

“…Previous studies have reported that the in vivo fiber photometry system could monitor the deep brain regions such as the ACC and prefrontal cortex, which comprise the pain matrix. [12][13][14][15][16] The results of these studies suggested that combination experiments of in vivo fiber photometry and behavior tests enable us to understand the interaction between changes of neuronal activities in pain matrix which contribute to affective component of pain and pain modulation, pain induced anxiety, or cognition of pain. On the other hand, we also monitored changes of calcium responses in the S1 cortex before and after administration of CFA, resulting that averaged amplitude of calcium responses in S1 were significantly increased 3 or 7 d after injection of CFA, in present study.…”

“…Recently, to identify the involvement of neuronal activities in the pain matrix in the development of chronic pain symptoms such as the sensory and affective components of pain, combined experiment with the behavior tests and in vivo calcium imaging using fiber photometry was applied. [12][13][14][15][16] The effects of drugs such as dexmedetomidine and duloxetine into ACC in chronic or inflammatory pain model mice with expressed GCaMP were measured using this combined experiment. As the results, administration of abovementioned drugs reduced the calcium responses of glutamatergic neurons in ACC and showed the anti-anxiety like behaviors.…”

“…12,14) Moreover, other previous studies also reported that the change of neuronal responses in parabrachial nucleus, ACC, or basolateral amygdala could be monitored using in vivo fiber photometry system in several pain model mice. 13,15,16) Thus, these combined experiment of this optical system and behavior tests seemed to be useful to understand the mechanism of causing chronic pain or to examine the effects of analgesics or pain treatments.…”

Remote automated delivery of mechanical stimuli coupled to brain recordings in behaving mice

“…Finally, we combine ARM stimulation with in vivo brain recording in the basolateral amygdala, an area that has been linked to encoding pain affect, unpleasantness, and negative emotion (Corder 2019, Meng 2022, Becker 2023, Tanimoto 2003). Although we focus on the amygdala as a proof-of-principle in this study, future studies could use this setup to combine ARM stimulation with behavior mapping and brain recordings in other cortical and sub-cortical areas implicated in pain (Meda, 2019, Chiang 2020, Tan 2021, Singh 2020, Okada 2021, Zhou & Li 2023, Li & Yang 2024, Chen & Sun 2023).…”

The neurobiology of pain and facial movements in rodents: Clinical applications and current research

“…Fiber photometry-based Ca2+ imaging revealed that dopamine, an important neuromodulator of pain-related behavior in mammals, increases the activity of the media prefrontal cortex neurons in the ventrolateral PAG and modulates responses to neuropathic pain by descending pain pathways ( 80 ). Likewise, fiber photometry showed that pathways involving glutamatergic neurons in the basolateral amygdala, insular cortex, and the mediodorsal thalamic nucleus upon inflammatory pain activate and modulate aversive responses to pain in mice ( 81 ). MRI and neural connectivity with ultra-high-field in rat models of knee chronic pain showed connectivity between ACC and subcortical structures, as well as a suppression of burrowing, a behavior associated with the presence of pain ( 82 ).…”