Distinct biomarker roles for HbA 1c and glycated albumin in patients with type 2 diabetes on hemodialysis

Hayashi, Akinori; Takano, Koji; Takasu, Masaki; Yoshino, Sonomi; Ogawa, Akifumi; Shichiri, Masayoshi

doi:10.1016/j.jdiacomp.2016.08.015

Cited by 35 publications

(32 citation statements)

References 43 publications

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“…Even the CVs of GA or HbA1c level, which adjusted the effect of mean glucose exposure, remained a significant risk factor for CAN. Therefore, the finding that the predictive power of GA variability for CAN development outperformed that of HbA1c variability was partially explained by GA’s shorter half-life, which enables it to reflect glucose excursions more accurately than does HbA1c [ 6 , 33 , 34 ]. The 2-year fluctuations in GA level were actually greater than those in HbA1c level in this study.…”

Section: Discussionmentioning

confidence: 99%

Glycated albumin and its variability as an indicator of cardiovascular autonomic neuropathy development in type 2 diabetic patients

Jun

Lee

et al. 2017

Cardiovasc Diabetol

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BackgroundWe investigated whether glycated albumin (GA) and its variability are associated with cardiovascular autonomic neuropathy (CAN) and further compared their associations with glycated hemoglobin (HbA1c).MethodsThis retrospective longitudinal study included 498 type 2 diabetic patients without CAN. CAN was defined as at least two abnormal results in parasympathetic tests or presence of orthostatic hypotension. The mean, standard deviation (SD), and coefficient of variance (CV) were calculated from consecutively measured GA (median 7 times) and HbA1c levels (median 8 times) over 2 years. Logistic regression analysis was used to compare the associations between CAN and GA- or HbA1c-related parameters. Receiver operating characteristic (ROC) curve analysis was used to compare the predictive power for CAN between GA- and HbA1c-related parameters.ResultsA total of 53 subjects (10.6%) developed CAN over 2 years. The mean, SD, and CV of GA or HbA1c were significantly higher in subjects with CAN. Higher mean GA and GA variability were associated with the risk of developing CAN, independent of conventional risk factors and HbA1c. In ROC curve analysis, the SD and CV of GA showed higher predictive value for CAN compared to the SD and CV of HbA1c, whereas the predictive value of mean GA did not differ from that of mean HbA1c. The mean, SD, and CV of GA showed additive predictive power to detect CAN development along with mean HbA1c.ConclusionsHigher serum GA and its variability are significantly associated with the risk of developing CAN. Serum GA might be a useful indicator for diabetic complications and can enhance HbA1c’s modest clinical prediction for CAN.Electronic supplementary materialThe online version of this article (doi:10.1186/s12933-017-0619-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Glycated albumin and its variability as an indicator of cardiovascular autonomic neuropathy development in type 2 diabetic patients

Jun

Lee

et al. 2017

Cardiovasc Diabetol

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“…Type 2 diabetic patients provided an additional serum sample at the time of scheduled blood sampling on the last morning of 72-h CGM recording using the CGMS system GOLD (Medtronic Minimed Inc. Northridge, CA)4647. They also received our routine systemic evaluation protocols for diabetic patients covered by universal health coverage system in Japan4348, which include laboratory tests, such as urinalysis, a complete blood count, serum biochemical analysis for more than 15 items, anti-glutamic acid decarboxylase antibody, glycated albumin, HbA1c, and fasting serum insulin.…”

Section: Methodsmentioning

confidence: 99%

Methionine sulfoxides in serum proteins as potential clinical biomarkers of oxidative stress

Suzuki

Kodera

Saito

et al. 2016

Sci Rep

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Oxidative stress contributes to the pathophysiology of a variety of diseases, and circulating biomarkers of its severity remains a topic of great interest for researchers. Our peptidomic strategy enables accurate and reproducible analysis of circulating proteins/peptides with or without post-translational modifications. Conventional wisdom holds that hydrophobic methionines exposed to an aqueous environment or experimental handling procedures are vulnerable to oxidation. However, we show that the mass spectra intensity ratio of oxidized to non-oxidized methionine residues in serum tryptic proteins can be accurately quantified using a single drop of human serum and give stable and reproducible results. Our data demonstrate that two methionine residues in serum albumin (Met-111 and Met-147) are highly oxidized to methionine sulfoxide in patients with diabetes and renal failure and in healthy smokers versus non-smoker controls. This label-free mass spectrometry approach to quantify redox changes in methionine residues should facilitate the identification of additional circulating biomarkers suitable for predicting the development or progression of human diseases.

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“…Diabetic patients received routine systemic evaluation, including serum biochemical analysis 40 , 41 . Patients were also evaluated for their glycemic excursion profiles using continuous glucose monitoring (CGM; CGMS® System Gold or Minimed iPro2® CGM, Medtronic Minimed) 33 , 44 . All 576 glucose values obtained at 5-min intervals during 48-h CGM recordings were used to calculate the mean glucose or the glycemic SD, and this was compared against the plasma free salusin-β level for each patient.…”

Section: Methodsmentioning

confidence: 99%

Identification and quantification of plasma free salusin-β, an endogenous parasympathomimetic peptide

Fujimoto

Hayashi

Kodera

et al. 2017

Sci Rep

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Salusin-β is an endogenous parasympathomimetic proatherosclerotic peptide. Salusin-β was initially predicted from bioinformatic analyses and later immunologically detected in human biofluids. However, elucidation of salusin-β bioactivity has faced enormous challenges because of its unique physicochemical characteristics that cause it to strongly adhere to laboratory apparatus materials. In the strictest sense, the discovery of bioactive peptides is not complete until their exact native sequences have been confirmed in the peripheral circulation. In this study, we determined the plasma molecular form and levels of free salusin-β to determine its pathophysiological significance. Ultra-high-yield enrichment and preseparation of non-tryptic human plasma was followed by LC-MS/MS, and full-length salusin-β and seven different endogenous fragment sequences were identified. We established a new ELISA that specifically detects plasma free salusin-β without cross-reacting with any of its identified endogenous fragments. Free salusin-β levels exhibited a profound early morning nadir and rapidly decreased in response to parasympathetic nervous augmentation. Our technical advance in plasma native peptide analysis successfully identified a hard-to-detect bioactive peptide, salusin-β, together with its formerly unrecognized fragments, and further suggests that conventional immunological measurements of target peptides may not be fully representative.

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Distinct biomarker roles for HbA 1c and glycated albumin in patients with type 2 diabetes on hemodialysis

Cited by 35 publications

References 43 publications

Glycated albumin and its variability as an indicator of cardiovascular autonomic neuropathy development in type 2 diabetic patients

Glycated albumin and its variability as an indicator of cardiovascular autonomic neuropathy development in type 2 diabetic patients

Methionine sulfoxides in serum proteins as potential clinical biomarkers of oxidative stress

Identification and quantification of plasma free salusin-β, an endogenous parasympathomimetic peptide

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