Distinct climatic regions drive antibiotic resistance genes dynamics across public parks and pristine soil ecosystems

Khalid, Muhammad Fasih; Liu, Xinxin; Zheng, Bang-Xiao; Su, Lantian; Kotze, D. Johan; Setälä, Heikki; Ali, Mehran; Rehman, Asad Ur; Rahman, Saeed-ur; Hu, Nan

doi:10.1016/j.jclepro.2023.137275

Cited by 9 publications

(3 citation statements)

References 109 publications

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“…2 ; LSD test, P < 0.05). The abundance of cphA3 (carbapenem resistance), PME-1 (carbapenem resistance), tcr3 (tetracycline antibiotic resistance), and AAC ( 3 )-VIIIa (aminoglycoside antibiotic resistance) in T1 was significantly higher than that of CK and T2 ( Fig. 2 ; LSD test, P < 0.05).…”

Section: Resultsmentioning

confidence: 93%

“…Antibiotic use in agriculture to increase crop productivity can prompt the emergence of antibiotic resistance in the pathogens, which may reduce the effectiveness of antimicrobial therapy for agriculture production ( 1 ). Agricultural soil is a natural reservoir that stores antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) from both native microbes and those introduced by anthropogenic activities ( 3 , 4 ). Besides, resistant bacteria can enter the plant root and migrate into the leaf and stem tissue, accelerating the spread of resistance genes in plants ( 1 ).…”

Section: Introductionmentioning

confidence: 99%

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What role do biocontrol agents with Mg ²⁺ play in the fate of antibiotic resistome and pathogenic bacteria in the phyllosphere?

Zhi,

Tan,

et al. 2024

mSystems

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The contamination of the plant phyllosphere with antibiotics and antibiotic resistance genes (ARGs), caused by application of antibiotics, is a significant environmental issue in agricultural management. Alternatively, biocontrol agents are environmentally friendly and have attracted a lot of interest. However, the influence of biocontrol agents on the phyllosphere resistome remains unknown. In this study, we applied biocontrol agents to control the wildfire disease in the Solanaceae crops and investigated their effects on the resistome and the pathogen in the phyllosphere by using metagenomics. A total of 250 ARGs were detected from 15 samples, which showed a variation in distribution across treatments of biocontrol agents (BA), BA with Mg 2+ (T1), BA with Mn 2+ (T2), and kasugamycin (T3) and nontreated (CK). The results showed that the abundance of ARGs under the treatment of BA-Mg 2+ was lower than that in the CK group. The abundance of cphA3 (carbapenem resistance), PME-1 (carbapenem resistance), tcr3 (tetracycline antibiotic resistance), and AAC (3)-VIIIa (aminoglycoside antibiotic resistance) in BA-Mg 2+ was significantly higher than that in BA-Mn 2+ ( P < 0.05). The abundance of cphA3 , PME_1, and tcr3 was significantly negatively related to the abundance of the phyllosphere pathogen Pseudomonas syringae ( P < 0.05). We also found that the upstream and downstream regions of cphA3 were relatively conserved, in which rpl , rpm, and rps gene families were identified in most sequences (92%). The Ka/Ks of cphA3 was 0 in all observed sequences, indicating that under the action of purifying selection, nonsynonymous substitutions are often gradually eliminated in the population. Overall, this study clarifies the effect of biocontrol agents with Mg 2+ on the distribution of the phyllosphere resistome and provides evolutionary insights into the biocontrol process. IMPORTANCE Our study applied metagenomics analysis to examine the impact of biocontrol agents (BAs) on the phyllosphere resistome and the pathogen. Irregular use of antibiotics has led to the escalating dissemination of antibiotic resistance genes (ARGs) in the environment. The majority of BA research has focused on the effect of monospecies on the plant disease control process, the role of the compound BA with nutrition elements in the phyllosphere disease, and the resistome is still unknown. We believe BAs are eco-friendly alternatives for antibiotics to combat the transfer of ARGs. Our results revealed that BA-Mg 2+ had a lower relative abundance of ARGs compared to the CK group, and the phyllosphere pathogen Pseudomonas syringae was negatively related to three specific ARGs, cphA3 , PME-1, and tcr3 . These three genes also present different Ka/Ks. We believe that the identification of the distribution and evolution modes of ARGs further elucidates the ecological role and facilitates the development of BAs, which will attract general interest in this field.

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Section: Resultsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

What role do biocontrol agents with Mg ²⁺ play in the fate of antibiotic resistome and pathogenic bacteria in the phyllosphere?

Zhi,

Tan,

et al. 2024

mSystems

View full text Add to dashboard Cite

show abstract

“…The extensive utilization of antibiotics, particularly during the COVID-19 pandemic, has substantially elevated their presence in water bodies through diverse routes, including pharmaceutical manufacturing effluents, hospital wastewater, and agricultural runoff, ultimately the contamination of aquatic ecosystems [2]. The excessive usage of antibiotics carries significant implications, including the emergence and spread of antibiotic-resistant pathogens, which poses a serious threat to public health [3]. To enhance the academic context, effective strategies for the removal of antibiotics from water are urgently required.…”

Section: Introductionmentioning

confidence: 99%

Enhanced antibiotic removal by waste coffee grounds prepared via water washing and KOH activation

Lee,

Kim

2023

Korean J. Chem. Eng.

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This paper proposed a method to remove amoxicillin (AMX) from waste coffee grounds (CGs) using environmentally friendly activated biochar-based adsorbents. KOH-activated adsorbents (activated CGs or ACGs) were prepared by washing with water to avoid using toxic chemicals. The maximum adsorption capacity and kinetics of the ACGs were evaluated using the Freundlich isotherm, Langmuir isotherm, adsorption density, and pseudo-second-order equation. ACG-4a (activated by KOH after washing with water) exhibited a significantly higher adsorption capacity (740.7 mg/g), whereas ACG-4b (activated by KOH after washing with NaOH) exhibited a lower capacity (549.5 mg/g). The specific surface area of the adsorbent is key to the adsorption performance. A comparison of the adsorbents revealed that the ACGs exhibited the highest AMX-adsorbing capacity. Their use has important environmental implications for water pollution mitigation and ecosystem preservation.

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Urbanization influences the indoor transfer of airborne antibiotic resistance genes, which has a seasonally dependent pattern

Zhao,

Liu,

Tan

et al. 2024

Environment International

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Distinct climatic regions drive antibiotic resistance genes dynamics across public parks and pristine soil ecosystems

Cited by 9 publications

References 109 publications

What role do biocontrol agents with Mg ²⁺ play in the fate of antibiotic resistome and pathogenic bacteria in the phyllosphere?

What role do biocontrol agents with Mg ²⁺ play in the fate of antibiotic resistome and pathogenic bacteria in the phyllosphere?

Enhanced antibiotic removal by waste coffee grounds prepared via water washing and KOH activation

Urbanization influences the indoor transfer of airborne antibiotic resistance genes, which has a seasonally dependent pattern

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Distinct climatic regions drive antibiotic resistance genes dynamics across public parks and pristine soil ecosystems

Cited by 9 publications

References 109 publications

What role do biocontrol agents with Mg 2+ play in the fate of antibiotic resistome and pathogenic bacteria in the phyllosphere?

What role do biocontrol agents with Mg 2+ play in the fate of antibiotic resistome and pathogenic bacteria in the phyllosphere?

Enhanced antibiotic removal by waste coffee grounds prepared via water washing and KOH activation

Urbanization influences the indoor transfer of airborne antibiotic resistance genes, which has a seasonally dependent pattern

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What role do biocontrol agents with Mg ²⁺ play in the fate of antibiotic resistome and pathogenic bacteria in the phyllosphere?

What role do biocontrol agents with Mg ²⁺ play in the fate of antibiotic resistome and pathogenic bacteria in the phyllosphere?