Distinct composition of human fetal HDL attenuates its anti-oxidative capacity

Sreckovic, Ivana; Birner‐Gruenberger, Ruth; Obrist, Britta; Stojaković, Tatjana; Scharnagl, Hubert; Holzer, Michael; Scholler, Monika; Philipose, Sonia; Marsche, Gunther; Lang, Uwe; Desoyé, Gernot; Wadsack, Christian

doi:10.1016/j.bbalip.2012.12.015

Cited by 51 publications

(52 citation statements)

References 66 publications

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“…Following the termination of the centrifuge run, fractions were collected and the absorbance at 280 nm was recorded. The amount recovered in each fraction was normalized to the total absorbance for all the recorded fractions ( 22,(32)(33)(34)(35), this may exacerbate the alteration of their recovered HDL; as in this report, we demonstrate that centrifugation of HDL at rotor speeds in excess of 30,000 rpm results in alteration to the recovered particle. Minimally altered HDL can be recovered from plasma samples using a reduced rotor speed of 15,000 rpm and a KBr-containing gradient in a similar time frame as the traditional ultracentrifuge-based isolation.…”

Section: Loss Of Protein From Hdlmentioning

confidence: 96%

Excessive centrifugal fields damage high density lipoprotein [S]

Munroe

Phillips

Schumaker

2015

Journal of Lipid Research

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This article is available online at http://www.jlr.org with centrifugation for 38 h to isolate HDL using a 40.3 or Ti70 rotor at у 40,000 rpm ( 4, 5 ). It was also John Gofman who proposed that elevated levels of lipoproteins cause atherosclerosis, the deposition of lipid in arteries and blood vessels that eventually leads to the premature deaths of so many people in the developed world ( 6 ). It was noticed that HDL could reverse this trend ( 7-9 ), which spurred an intense research focus on the mechanism of HDL as an atheroprotective particle ( 10-15 ), as well as identifying its other functions (16)(17)(18)(19)(20).Few reports have attempted to characterize the stability of lipoproteins in a centrifugal fi eld. Kunitake and Kane ( 21 ) studied human lipoproteins and found that apoAI was lost during each spin until one-third of the apoAI was lost with fi ve spins. Additionally, Ståhlman et al. ( 22 ) demonstrated that using reduced ionic strength solutions allowed for greater recovery of exchangeable apolipoproteins. Increased ionic strength solutions were found to improve the retention of apoAI on the HDL ( 21 ), whereas this decreased the retention of the exchangeable apolipoproteins ( 22 ). Thus, for different apolipoproteins, their mechanism of dissociation from HDL differs. The assembly of HDL is hinted at by less disruptive characterization methods, such as nondenaturing gradient gel electrophoresis. Li et al. ( 23 ) discovered that native gel electrophoresis produced a series of 14 distinct HDL peaks, which they labeled in the order in which they appeared, although most individuals only have about six. LeBoeuf et al. ( 24 ), characterized mouse lipoproteins from a variety of inbred mouse strains and found that mouse lipoproteins were roughly similar in size, although there were differences in the electrophoretic position of the major peak. Thus, depending upon the method of isolation, the pattern of lipoproteins recovered differs.As there are many contemporary publications that utilize high ultracentrifuge rotor speeds, from 65,000 to 120,000 rpm, in order to reduce the time frame needed to isolate lipoproteins ( 25-31 ), and many characterizations of HDL utilize material which was purifi ed via ultracentrifugation Abstract HDL is typically isolated ultracentrifugally at 40,000 rpm or greater, however, such high centrifugal forces are responsible for altering the recovered HDL particle. We demonstrate that this damage to HDL begins at approximately 30,000 rpm and the magnitude of loss increases in a rotor speed-dependent manner. The HDL is affected by elevated ultracentrifugal fi elds resulting in a lower particle density due to the shedding of associated proteins. To circumvent the alteration of the recovered HDL, we utilize a KBr-containing density gradient and a lowered rotor speed of 15,000 rpm to separate the lipoproteins using a single 96 h centrifugation step. This recovers the HDL at two density ranges; the bulk of the material has a density of about 1.115 g/ml, while lessor amounts of material ...

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Section: Loss Of Protein From Hdlmentioning

confidence: 96%

Excessive centrifugal fields damage high density lipoprotein [S]

Munroe

Phillips

Schumaker

2015

Journal of Lipid Research

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“…Detailed proteomic methods have been previously published [11]. The LC-MS/MS data were analyzed by searching the human NCBI database.…”

Section: Shotgun Proteomics By Lc-ms/ms and Lc-ms/ms Data Analysismentioning

confidence: 99%

“…Due to the small plasma volume obtained from the umbilical cord, neonatal HDLs were isolated by an adapted ultracentrifugation method [11]. HDL was centrifuged in a TL-100 Tabletop centrifuge (Beckman Coulter, CA, USA).…”

Section: Isolation and Characterization Of Hdlmentioning

confidence: 99%

“…Total lysed maternal-and their corresponding neonatal HDLproteins (15 μg) from control and GDM representative subjects (n = 3), were separated by 4-20% gels (Thermo Scientific, Rockford, USA) and analyzed as described previously [11]. Specific proteins were immunodetected by using antibodies for albumin (1:2000, abcam -ab83465 Cambridge, UK) apoA-1 (1:3000, Novus Biologicals, NB100-65491, Littleton, CO, USA), apoA-2 (1:1000, abcamab54796), apoE (1:1000, abcam -ab1906), apoM (1:1000, abcamab57471), PON1 (1:1000, abcam -ab24261) and SAA1 (1:1000, abcam -ab50232).…”

Section: Western Blottingmentioning

confidence: 99%

“…Recently, we showed that neonatal HDL differs from maternal with respect to its proteome, size and function [11]. Neonatal and adult HDL undergoes constant remodeling, that is dependent on the metabolic status of the mother and/or the fetus [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity

Sreckovic

Birner‐Gruenberger

Besenboeck

et al. 2014

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Exploring the Lifeline: Unpacking the Complexities of Placental Vascular Function in Normal and Preeclamptic Pregnancies

Schliefsteiner,

Wadsack,

Allerkamp

2024

Comprehensive Physiology

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The proper development and function of the placenta are essential for the success of pregnancy and the well‐being of both the fetus and the mother. Placental vascular function facilitates efficient fetal development during pregnancy by ensuring adequate gas exchange with low vascular resistance. This review focuses on how placental vascular function can be compromised in the pregnancy pathology preeclampsia, and conversely, how placental vascular dysfunction might contribute to this condition. While the maternal endothelium is widely recognized as a key focus in preeclampsia research, this review emphasizes the importance of understanding how this condition affects the development and function of the fetal placental vasculature. The placental vascular bed, consisting of microvasculature and macrovasculature, is discussed in detail, as well as structural and functional changes associated with preeclampsia. The complexity of placental vascular reactivity and function, its mediators, its impact on placental exchange and blood distribution, and how these factors are most affected in early‐onset preeclampsia are further explored. These factors include foremost lipoproteins and their cargo, oxygen levels and oxidative stress, biomechanics, and shear stress. Challenges in studying placental pathophysiology are discussed, highlighting the necessity of innovative research methodologies, including ex vivo experiments, in vivo imaging tools, and computational modeling. Finally, an outlook on the potential of drug interventions targeting the placental endothelium to improve placental vascular function in preeclampsia is provided. Overall, this review highlights the need for further research and the development of models and tools to better understand and address the challenges posed by preeclampsia and its effects on placental vascular function to improve short‐ and long‐term outcomes for the offspring of preeclamptic pregnancies. © 2024 American Physiological Society. Compr Physiol 14:5763‐5787, 2024.

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Distinct composition of human fetal HDL attenuates its anti-oxidative capacity

Cited by 51 publications

References 66 publications

Excessive centrifugal fields damage high density lipoprotein [S]

Excessive centrifugal fields damage high density lipoprotein [S]

Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity

Exploring the Lifeline: Unpacking the Complexities of Placental Vascular Function in Normal and Preeclamptic Pregnancies

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