Distinct contributions of one Fe- and two Cu/Zn-cofactored superoxide dismutases to antioxidation, UV tolerance and virulence of Beauveria bassiana

Li, Fang; Shi, Han-Qiang; Ying, Sheng‐Hua; Feng, Ming‐Guang

doi:10.1016/j.fgb.2014.09.006

Cited by 63 publications

(46 citation statements)

References 43 publications

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“…Total activities of SODs and catalases are crucial for the responses of B . bassiana to menadione and H 2 O 2 30–32 and were linearly correlated with antioxidant responses of all tested strains in this study. Fungal cell wall integrity is required for various cellular events, such as protein glycosylation, glycosyl-phoshatidylinositol (GPI) anchor biosynthesis, quality control of secretory proteins and delivery of cell wall components 33, 34 .…”

Section: Discussionsupporting

confidence: 56%

Differential Roles for Six P-Type Calcium ATPases in Sustaining Intracellular Ca2+ Homeostasis, Asexual Cycle and Environmental Fitness of Beauveria bassiana

Wang

Zhu

Ying

et al. 2017

Sci Rep

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A global insight into the roles of multiple P-type calcium ATPase (CA) pumps in sustaining the life of a filamentous fungal pathogen is lacking. Here we elucidated the functions of five CA pumps (Eca1, Spf1 and PmcA/B/C) following previous characterization of Pmr1 in Beauveria bassiana, a fungal insect pathogen. The fungal CA pumps interacted at transcriptional level, at which singular deletions of five CA genes depressed eca1 expression by 76–98% and deletion of spf1 resulted in drastic upregulation of four CA genes by 36–50-fold. Intracellular Ca2+ concentration increased differentially in most deletion mutants exposed to the stresses of Ca2+, EDTA chelator, and/or endoplasmic reticulum and calcineurin inhibitors, accompanied with their changed sensitivities to not only the mentioned agents but also Fe2+, Cu2+ and Zn2+. Liquid culture acidification was delayed in the Δspf1, Δpmr1 and ΔpmcA mutants, coinciding well with altered levels of their extracellular lactic and oxalic acids. Moreover, all deletion mutants showed differential defects in conidial germination, vegetative growth, conidiation capacity, antioxidant activity, cell wall integrity, conidial UV-B resistance and/or virulence. Our results provide the first global insight into differential roles for six CA pumps in sustaining intracellular Ca2+ level, asexual cycle and environmental fitness of B. bassiana.

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Section: Discussionsupporting

confidence: 56%

Differential Roles for Six P-Type Calcium ATPases in Sustaining Intracellular Ca2+ Homeostasis, Asexual Cycle and Environmental Fitness of Beauveria bassiana

Wang

Zhu

Ying

et al. 2017

Sci Rep

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“…Previously, several catalases and SODs in Beauveria bassiana, a fungal entomopathogen widely applied in arthropod pest control, were confirmed contributing differentially to thermotolerance, UV resistance and/or virulence, which are largely determinant to the potential (Xie et al, , 2013Wang et al, 2013;Li et al, 2014). Our preliminary analysis of B. bassiana genome (Xiao et al, 2012) revealed an existence of six Trx homologs (Trx1-6).…”

Section: Introductionmentioning

confidence: 58%

Subcellular localization of six thioredoxins and their antioxidant activity and contributions to biological control potential in Beauveria bassiana

Zhang

Tang

Ying

et al. 2015

Fungal Genetics and Biology

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“…Similarly, the marked reduction in total SOD activity in the Δtrr1 mutant was also restored only by overexpression of trx1 or trx6 in the Δtrr1 background. These results indicate that the backup Trr2 could be activated by the function loss of Trr1 to reduce Trx1 and Trx6 in the redox system and hence could completely remove the suppressive effect of deleted trr1 on the activities of SODs characterized previously Li et al 2015) and PODs to be explored. In B. bassiana, two MnSODs localized in the cytoplasm and mitochondria, respectively, have been shown to dominate total SOD activity, which was responsible for cellular response to menadione more than to H 2 O 2 and a large part of variability in intracellular ROS level .…”

Section: Discussionmentioning

confidence: 89%

Distinct roles of two cytoplasmic thioredoxin reductases (Trr1/2) in the redox system involving cysteine synthesis and host infection of Beauveria bassiana

Zhang

Tang

Ying

et al. 2016

Appl Microbiol Biotechnol

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Two thioredoxin (Trx) reductases (Trr1/2) are known to play overlapping roles in the yeast Trx-Trr redox system but are generally unexplored in filamentous fungi, which possess multiple Trx homologues. This study seeks to characterize the functions of Trr1 and Trr2 in Beauveria bassiana, a filamentous fungal insect pathogen, and to probe their Trx partners. Both Trr1 and Trr2 were evidently localized in the cytoplasm of B. bassiana, unlike the two yeast homologues that have been reported to localize in the cytoplasm and mitochondria, respectively. Most of the six trx genes were greatly upregulated at the transcriptional level in the absence of trr1 instead of trr2 in B. bassiana, in which the trr1/2 double deletion failed in many attempts. Deletion of trr1 resulted in increased Trx activity, severe cysteine auxotrophy, and drastically reduced activities of peroxidases and superoxide dismutases under normal or oxidative conditions despite little change in catalase activity. Such changes disappeared in the absence of trr2 and were completely restored by complementation of trr1/2 or overexpression of trx1/6 in the Δtrr1 mutant, but were not restored at all by overexpression of trx2/3/4/5 or trr2 in the same mutant. All of these mutants exhibited similar trends of changes in the antioxidant response, conidiation, germination, thermotolerance, UV-B resistance, and virulence. Taken together, the findings indicate that Trr1 could reduce Trx2-5 and hence dominate the intracellular redox state, profoundly affecting the potential of B. bassiana against arthropod pests. Trr2 could reduce Trx1/6 but function only in the absence of Trr1.

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Distinct contributions of one Fe- and two Cu/Zn-cofactored superoxide dismutases to antioxidation, UV tolerance and virulence of Beauveria bassiana

Cited by 63 publications

References 43 publications

Differential Roles for Six P-Type Calcium ATPases in Sustaining Intracellular Ca2+ Homeostasis, Asexual Cycle and Environmental Fitness of Beauveria bassiana

Differential Roles for Six P-Type Calcium ATPases in Sustaining Intracellular Ca2+ Homeostasis, Asexual Cycle and Environmental Fitness of Beauveria bassiana

Subcellular localization of six thioredoxins and their antioxidant activity and contributions to biological control potential in Beauveria bassiana

Distinct roles of two cytoplasmic thioredoxin reductases (Trr1/2) in the redox system involving cysteine synthesis and host infection of Beauveria bassiana

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