Distinct dynamics of lipid accumulation by porcine cumulus cells during in vitro maturation with follicular fluid of low and high fatty acid contents

Małyszka, Natalia; Pawlak, Piotr; Cieślak, Adam; Szkudelska, K.; Lechniak, D.

doi:10.1016/j.theriogenology.2022.10.015

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…The causes of these poor results may be its role at the molecular level. It has been reported that fatty acids in porcine FF (pFF) affected the lipid metabolism of cumulus–oocyte complexes (COCs) [ 11 ]. Moreover, Pawlak et al suggested that the competence of porcine oocytes and embryos depends on the fatty acid profiles of FF collected from prepubertal and cyclic gilts [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Porcine Follicular Fluid-Derived Exosome: The Pivotal Material for Porcine Oocyte Maturation in Lipid Antioxidant Activity

Kim

Lee

2023

IJMS

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Several studies have examined exosomes derived from porcine follicular fluid (FF), but few have reported their application in controlled experiments. The main concern in the field of embryology may be that controlled conditions, such as using a defined medium intermittently, cause poor results in mammalian oocyte maturation and embryo development. The first reason is the absence of the FF, which copes with the majority of the processes emerging in oocytes and embryos. Therefore, we added exosomes derived from porcine FF to the maturation medium of porcine oocytes. For morphological assessment, cumulus cell expansion and subsequent embryonic development were evaluated. Moreover, several stainings, such as glutathione (GSH) and reactive oxygen species (ROS), fatty acid, ATP, and mitochondrial activity, as well as evaluations of gene expression and protein analysis, were used for the functional verification of exosomes. When the oocytes were treated with exosomes, the lipid metabolism and cell survival of the oocytes were fully recovered, as well as morphological evaluations compared to the porcine FF-excluded defined medium. Therefore, controlled experiments may produce reliable data if the exosomes are treated with the desired amounts, and we suggest applying FF-derived exosomes to promote experimental data when performing controlled experiments in embryology.

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Section: Introductionmentioning

confidence: 99%

Porcine Follicular Fluid-Derived Exosome: The Pivotal Material for Porcine Oocyte Maturation in Lipid Antioxidant Activity

Kim

Lee

2023

IJMS

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Feline cumulus cells and oocytes show massive accumulation of lipid droplets and upregulation of PLIN2 expression after in vitro maturation

Sowinska,

Lechtanska,

Greczka

et al. 2025

Theriogenology

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6‐Gingerol and Astaxanthin Mitigate the Effects of Stearic Acid in Pig Oocyte Maturation

Saeed,

Sabir,

et al. 2024

Reprod Domestic Animals

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Elevated non‐esterified fatty acids (NEFAs), particularly stearic acid (SA), have a deleterious effect on oocyte maturation, leading to developmental damage and reproductive issues. High SA levels disrupt metabolic processes, inducing lipotoxicity that impairs oocyte quality and contributes to reproductive failures through early embryonic losses. This research investigates the lipotoxic effects of SA and assesses the protective potential of 6‐Gingerol (6‐G) and Astaxanthin (AX) on porcine oocytes during in vitro maturation (IVM). Herein, 6100 cumulus‐oocyte complexes (COCs) were exposed to various concentrations of SA (25–250 μM) to elucidate the concentration‐dependent effect on oocyte viability, polar body extrusion (PBE) and cumulus cell expansion index (CCEI). However, the efficacy of 6‐G (5–15 μM) and AX (2.5 μM) in combination with SA at 150 μM (SA6) concentration was evaluated to mitigate these adverse effects. The results indicated that SA6 substantially reduced oocyte viability, PBE and CCEI, demonstrating its toxic impact on oocyte developmental competence (p < 0.0001). Moreover, treatment with antioxidants such as SA6 + 6‐G (10 μM) and SA6 + AX showed a considerable increase in viability and PBE compared to SA6 alone (p < 0.05). These findings demonstrate the importance of lipid metabolism in oocyte health, where dysregulation impairs reproductive capacity. Both 6‐G and AX protected against lipotoxicity induced by SA6 while enhancing lipid homeostasis and the anti‐oxidative defences necessary for maintaining cellular integrity. This study finds substantial evidence that optimising the microenvironment with specific antioxidants can improve oocyte quality and provide invaluable knowledge in reproductive technologies and fertility treatments.

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Distinct dynamics of lipid accumulation by porcine cumulus cells during in vitro maturation with follicular fluid of low and high fatty acid contents

Cited by 3 publications

References 17 publications

Porcine Follicular Fluid-Derived Exosome: The Pivotal Material for Porcine Oocyte Maturation in Lipid Antioxidant Activity

Porcine Follicular Fluid-Derived Exosome: The Pivotal Material for Porcine Oocyte Maturation in Lipid Antioxidant Activity

Feline cumulus cells and oocytes show massive accumulation of lipid droplets and upregulation of PLIN2 expression after in vitro maturation

6‐Gingerol and Astaxanthin Mitigate the Effects of Stearic Acid in Pig Oocyte Maturation

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