Distinct Effects of Platelet-Rich Plasma and BMP13 on Rotator Cuff Tendon Injury Healing in a Rat Model

Lamplot, Joseph D.; Angeline, Michael E.; Angeles, Jovito; Beederman, Maureen; Wagner, Eric R.; Rastegar, Farbod; Scott, Bryan L.; Skjong, Christian; Mass, Daniel P.; Kang, Richard W.; Ho, Sherwin S.W.; Shi, Lewis L.

doi:10.1177/0363546514547171

Cited by 42 publications

(39 citation statements)

References 61 publications

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“…More importantly, the maximum load and stiffness were increased significantly in the H-dose group from 8 to 16 weeks compared with the other groups in our study. A similar finding was described by Lamplot et al 24 , who investigated the upregulated fibronectin expression of bone morphogenetic protein 13 (BMP13, a growth factor of TGF). Results showed that BMP13 promoted tendon healing, because of its superior biological factor, and reduced the incidence of re-tearing.…”

Section: ■ Discussionsupporting

confidence: 78%

Biomechanic and histologic analysis of fibroblastic effects of tendon-to-bone healing by transforming growth factor β1 (TGF-β1) in rotator cuff tears

Zhang

Liu

2017

Acta Cir. Bras.

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Purpose: To evaluate the effect of transforming growth factor β 1 (TGF-β 1 ) on tendon-to-bone reconstruction of rotator cuff tears. Methods: Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain. Collagen type III was observed by picric acid sirius red staining under polarized light. The level of insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) was measured by the enzyme-linked immunosorbent assay (ELISA) method. Results: Collagen type III of the H-dose group had a significant difference in histology structure compared with the L-dose group (P<0.05). The maximum load and stiffness decreased significantly in the control group compared with the values of the L-dose and H-dose groups. The stiffness among the three groups differed significantly at the same postoperative time (P<0.05). Interestingly, progressive reestablishment of collagen type III affected tendon-tobone healing significantly in the later stages. Conclusion: The H-dose was associated with an increased collagen type III morphology stimulated by TGF-β 1.

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Section: ■ Discussionsupporting

confidence: 78%

Biomechanic and histologic analysis of fibroblastic effects of tendon-to-bone healing by transforming growth factor β1 (TGF-β1) in rotator cuff tears

Zhang

Liu

2017

Acta Cir. Bras.

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“…5 The transduction of mice tenocytes with adeno-virus expressing BMP-13 increased the expression of genes involved in tendon healing, stress to failure and stiffness. 21 Our study adds to this body of work, by demonstrating for the first time the regenerative potential of BMP-13 in human IVD cells by inducing matrix production.…”

Section: Bmp-13 In Human Intervertebral Discmentioning

confidence: 72%

Localization of bone morphogenetic protein 13 in human intervertebral disc and its molecular and functional effects in vitro in 3D culture

Gulati

Chung

Wei

et al. 2015

Journal Orthopaedic Research

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Our laboratory has demonstrated that bone morphogenetic protein 13 prevented the effects of annular injury in an ovine model, maintaining intervertebral disc height, cell numbers and increasing extracellular matrix production compared to degenerated controls. The present study sought to examine the molecular effects of bone morphogenetic protein 13 on human degenerated disc cells and localize its expression in both human degenerate and scoliotic disc tissue. Effect of bone morphogenetic protein 13 on human derived nucleus pulposus, annulus fibrosus and endplate cells cultured in alginate beads was evaluated by changes in proteoglycan and collagen content. Migratory potential of disc cells towards bone morphogenetic protein 13 was also examined. Bone morphogenetic protein 13 induced significant proteoglycan accumulation in nucleus (18%), annulus (21%) and endplate (23%) cells cultured in alginate beads (p < 0.05) compared to controls. Further bone morphogenetic protein 13 increased collagen I and II protein expression in nucleus and endplate cells. Nucleus cells displayed a significant chemotactic response towards bone morphogenetic protein 13. The endogenous expression of bone morphogenetic protein 13 in degenerate disc tissue was not different to scoliotic disc. Bone morphogenetic protein 13 has the potential to enhance extracellular matrix accumulation and induce cell migration in certain disc cells. Keywords: bone morphogenetic protein 13; human intervertebral disc; low back pain; cell migration; intervertebral disc degeneration Chronic low back pain is strongly associated with intervertebral disc (IVD) degeneration. IVD consists of a central gelatinous nucleus pulposus (NP), surrounded circumferentially by annulus fibrosus (AF) and separated from the vertebral bodies by cartilaginous endplates (EPs). During IVD degeneration, cellular viability is decreased and the gel-like composition of NP becomes fibrous. Additionally, there is increasing irregularity and bifurcation of the lamellar structure of AF. 1Biological therapies aim to retard IVD degeneration and re-establish its biological function by restoring cellular viability and extracellular matrix (ECM). Growth factors such as bone morphogenetic proteins (BMPs), members of transforming growth factor beta (TGF-b) superfamily, play a critical role in stimulation of ECM formation and maintenance of cell numbers. The effect of BMPs on degenerate IVDs in vitro and in vivo has been extensively studied, 2 however, the role of BMP-13 in disc regeneration has not been explored. BMP-13, the human homologue of murine growth and differentiation factor 6, 3 has been highly associated with the stimulation of ECM production. BMP-13 induced aggrecan gene expression and proteoglycan (PG) synthesis in mouse mesenchymal cell line 4 and human articular chondrocytes.5 BMP-13 also stimulated gene expression of collagen II in mouse mesenchymal cell line 4 and collagen synthesis in juvenile bovine articular cartilage explants. 6 Moreover, Zhang et al.have demonstrated t...

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“…Due to its role in angiogenesis, it was proposed that it plays a critical role in tendon healing [130]. Recently, an in vivo model of rotator cuff healing showed that VEGF improved biomechanical properties of repaired tissue [131]. However, in two clinical studies, VEGF expression was associated with motion pain and synovial proliferation in patients with impingement syndrome, while a correlation with diabetes was also reported [132, 133].…”

Section: Growth Factorsmentioning

confidence: 99%

Effectiveness of Biologic Factors in Shoulder Disorders

Giotis¹,

Aryaei²,

Vasilakakos³

et al. 2017

TOORTHJ

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Background:Shoulder pathology can cause significant pain, discomfort, and loss of function that all interfere with activities of daily living and may lead to poor quality of life. Primary osteoarthritis and rotator cuff diseases with its sequalae are the main culprits. Management of shoulder disorders using biological factors gained an increasing interest over the last years. This interest reveals the need of effective treatments for shoulder degenerative disorders, and highlights the importance of a comprehensive and detailed understanding of the rapidly increasing knowledge in the field.Methods:This study will describe most of the available biology-based strategies that have been recently developed, focusing on their effectiveness in animal and clinical studies.Results:Data from in vitro work will also be briefly presented; in order to further elucidate newly acquired knowledge regarding mechanisms of tissue degeneration and repair that would probably drive translational work in the next decade. The role of platelet rich-plasma, growth factors, stem cells and other alternative treatments will be described in an evidence-based approach, in an attempt to provide guidelines for their clinical application. Finally, certain challenges that biologic treatments face today will be described as an initiative for future strategies.Conclusion:The application of different growth factors and mesenchymal stem cells appears as promising approaches for enhancing biologic repair. However, data from clinical studies are still limited, and future studies need to improve understanding of the repair process in cellular and molecular level and evaluate the effectiveness of biologic factors in the management of shoulder disorders.

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Distinct Effects of Platelet-Rich Plasma and BMP13 on Rotator Cuff Tendon Injury Healing in a Rat Model

Abstract: While PRP is used in the clinical setting, BMP13 may be explored as a superior biofactor to improve rotator cuff tendon healing and reduce the incidence of retears.

Cited by 42 publications

References 61 publications

Biomechanic and histologic analysis of fibroblastic effects of tendon-to-bone healing by transforming growth factor β1 (TGF-β1) in rotator cuff tears

Biomechanic and histologic analysis of fibroblastic effects of tendon-to-bone healing by transforming growth factor β1 (TGF-β1) in rotator cuff tears

Localization of bone morphogenetic protein 13 in human intervertebral disc and its molecular and functional effects in vitro in 3D culture

Effectiveness of Biologic Factors in Shoulder Disorders

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