Distinct effects of recombinant tenascin-C domains on neuronal cell adhesion, growth cone guidance, and neuronal polarity

Abstract

Using a set of recombinantly expressed proteins, distinct domains of the mouse extracellular matrix glycoprotein tenascin‐C, hereafter called tenascin, have been identified to confer adhesion, anti‐adhesion, and changes in morphology of neuronal cells. In short‐term adhesion assays (1 hr), cerebellar and hippocampal neurons adhered to several domains, encompassing the fibronectin type III‐like (FN III) repeats 1–2 and 6–8, as well as to the alternatively spliced FN III repeats and to tenascin itself. Although no short‐term adhesion to the EGF repeats containing fragment could be detected under the conditions used, it was anti‐adhesive for neuronal cell bodies and repellent for growth cone advance and neuritogenesis. FN III repeats 3–5 were repellent only for growth cones but not for neuronal cell bodies. Neurite outgrowth promoting activities at early stages and induction of a polarized neuronal morphology at later stages of differentiation were associated with the EGF repeats and the FN III repeats 6–8. These observations suggest differential effects of particular domains of the tenascin molecule on distinct cellular compartments, i.e., cell body, axon and dendrite, and existence of multiple neuronal receptors with distinct intracellular signaling features. © 1996 Wiley‐Liss, Inc.

Nogo‐66 and myelin‐associated glycoprotein (MAG) inhibit the adhesion and migration of Nogo‐66 receptor expressing human glioma cells

“…U87MG cells were plated on recombinant GST and the respective GST fusion proteins (25–100 µg/mL solution in 2 µL‐sized spots) that are dried down onto poly‐ l ‐lysine (PLL)‐treated dishes which have been pre‐coated with a thin layer of nitrocellulose. Cells were incubated at 37°C before fixation and staining with Commassie Blue after various time points (Dorries et al . 1996).…”

Tenascin Supports Lymphocyte Rolling

“…The fibrinogen domain mediates adhesion of fibroblasts via a cell surface proteoglycan (5), endothelial cells via an integrin (35), and a variety of other cells (46). In addition to these two sites, neurons can bind to FN-III domains 1-2, 7-8, and the alternatively spliced domains (22,30,46).…”

Recognition molecules and neural repair

“…Thus, both neuronal cell bodies and axonal growth cones appear to be able to cruise without impediment through a homogenous tenascin‐C territory in vivo . The domains in tenascin‐C and ‐R involved in promotion and inhibition of neurite outgrowth and neuronal migration (Dorries et al. 1996; Xiao et al.…”