Distinct Epidermal Keratinocytes Respond to Extremely Low-Frequency Electromagnetic Fields Differently

Huang, Chao-Ying; Chuang, Chun‐Yu; Shu, Wun‐Yi; Chang, Cheng-Wei; Chen, Chaang-Ray; Fan, T.C.; Hsu, Ian C.

doi:10.1371/journal.pone.0113424

Cited by 11 publications

(6 citation statements)

References 47 publications

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“…The exposure to electromagnetic fields (EMF) can lead to different biological effects depending on the intensity, frequency, dose, exposure time and cell type. In fact, recent evidences showed that different keratinocyte lines may respond differently to ELF‐EMF, and our early reports showed that on HaCaT keratinocytes cells line the ELF‐EMF (50 Hz, 1mT) triggers a change in the gene expression profile suggesting a switch towards an increase of “Proliferative” and “Migration” function and modulation of inflammatory mediators …”

Section: Discussionmentioning

confidence: 97%

“…In fact, recent evidences showed that different function and modulation of inflammatory mediators. 12,19,21 It is well known that keratinocytes play an important role in covering the wound bed by migration and proliferation, and that at the migrating front of re-epithelization, during wound healing. They are the predominant source of MMPs and their timing of expression, activation and regulation is fundamental for an efficacious healing.…”

Section: Discussionmentioning

confidence: 99%

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Extremely low‐frequency electromagnetic fields accelerates wound healing modulating MMP‐9 and inflammatory cytokines

et al. 2018

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Extremely low‐frequency electromagnetic fields accelerates wound healing modulating MMP‐9 and inflammatory cytokines

et al. 2018

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“…Moreover, Huang et al observed that EMF may also differently affect the same type of cells i.e. epidermis keratinocytes [ 34 ]. We also confirmed this observation.…”

Section: Discussionmentioning

confidence: 99%

Fas/FasL pathway and cytokines in keratinocytes in atopic dermatitis – Manipulation by the electromagnetic field

et al. 2018

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BackgroundAtopic dermatitis (AD) is one of the most frequent skin diseases. Changes of the keratinocytes functionality play a major role in the development of AD. For example, activation of the Fas (CD95)/FasL (CD178) pathway in AD does not lead to extensive apoptosis in skin. Binding of the Fas receptor to its protein ligand—FasL, which are present on the (AD)-modified keratinocytes, should result in the sequential induction of cell death, but there is no evidence of extensive apoptosis of these cells. This suggests that non-apoptotic mechanism of Fas/FasL pathway is commonly encountered, although not examined in the case of AD, phenomenon. An electromagnetic field, which was used to influence cultured cells in this study, can modulate proliferation, apoptosis, differentiation, and metabolism in various cells.ObjectiveHere, we evaluate the possibility to manipulate the immune activation of AD keratinocytes and their response to the electromagnetic field, which was not tested before.MethodsKeratinocytes isolated from the skin of healthy subjects (n = 20) and patients with atopic dermatitis (n = 20) as well as HaCaT and PCS-200-010 cell were exposed to the 900 MHz electromagnetic field for 60 minutes. Cytometric analysis of viability, Fas/FasL, p-ERK, p-p38 and p-JNK expression and Luminex analysis of cytokine concentration were performed in two-time points: 4 and 24 hours after the exposition.ResultsThis research has shown upregulated Fas, FasL, p-ERK, p-p38, and p-JNK expression along with increased cytokine secretion (IL-1β, IL-4, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-31 and TNFα) by keratinocytes derived from the skin of patients with the AD when compared with healthy control. Exposure of keratinocyte cultures obtained from AD patients to EMF resulted in a decrease of 1β, IL-4, IL-10, IL-12, I L-13, IL-17, IL-31 and TNFα levels. Keratinocytes derived from the skin of AD patients are characterized by elevated Fas and FasL expression when compared to healthy control.ConclusionApoptotic and nonapoptotic activation of the Fas/FasL-dependent signaling pathway may play a significant role in the pathogenesis of AD, by adjusting the local cytokine and chemokine environment at the site of inflammation. Moreover, the electromagnetic field exhibits strong immunomodulatory effects on AD-modified keratinocytes.

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“…A 50 Hz MF of 1 mT induced a proliferative and survival advantage even in malignant tumor cells by activating antioxidative and detoxification cytoprotective pathways [ 14 ]. In contrast, 50 Hz MFs ranging 0.9–4.8 mT inhibited osteoblast proliferation in a non-dose-dependent manner [ 15 ], and a 60 Hz MF at 1.5 mT inhibited epidermal keratinocyte proliferation [ 16 ]. Other studies reported that 50/60 Hz MFs ranging 0.5–3 mT did not affect either cell proliferation or cell cycle [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

A 50 Hz magnetic field affects hemodynamics, ECG and vascular endothelial function in healthy adults: A pilot randomized controlled trial

et al. 2021

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Application of exposure to 50/60 Hz magnetic fields (MFs) has been conducted in the treatment of muscle pain and fatigue mainly in Japan. However, whether MFs could increase blood flow leading to muscle fatigue recovery has not been sufficiently tested. We investigated the acute effects of a 50 Hz sinusoidal MF at Bmax 180 mT on hemodynamics, electrocardiogram, and vascular endothelial function in healthy young men. Three types of regional exposures to a 50 Hz MF, i.e., forearm, upper arm, or neck exposure to MF were performed. Participants who received three types of real MF exposures had significantly increased ulnar arterial blood flow velocity compared to the sham exposures. Furthermore, after muscle loading exercise, MF exposure recovered hemoglobin oxygenation index values faster and higher than sham exposure from the loading condition. Moreover, participants who received real MF exposure in the neck region had significantly increased parasympathetic high-frequency activity relative to the sham exposure. The MF exposure in the upper arm region significantly increased the brachial artery flow-mediated dilation compared to the sham exposure. Computer simulations of induced in situ electric fields indicated that the order-of-magnitude estimates of the peak values were 100–500 mV/m, depending on the exposure conditions. This study provides the first evidence that a 50 Hz MF can activate parasympathetic activity and thereby lead to increase vasodilation and blood flow via a nitric oxide-dependent mechanism. Trial registration: UMIN Clinical Trial Registry (CTR) UMIN000038834. The authors confirm that all ongoing and related trials for this drug/intervention are registered.

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Distinct Epidermal Keratinocytes Respond to Extremely Low-Frequency Electromagnetic Fields Differently

Cited by 11 publications

References 47 publications

Extremely low‐frequency electromagnetic fields accelerates wound healing modulating MMP‐9 and inflammatory cytokines

Extremely low‐frequency electromagnetic fields accelerates wound healing modulating MMP‐9 and inflammatory cytokines

Fas/FasL pathway and cytokines in keratinocytes in atopic dermatitis – Manipulation by the electromagnetic field

A 50 Hz magnetic field affects hemodynamics, ECG and vascular endothelial function in healthy adults: A pilot randomized controlled trial

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