Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research

Zomer, Helena Debiazi; Roballo, Kelly Cristine Santos; Lessa, Thais Borges; Bressan, Fabiana Fernandes; Gonçalves, Natália N; Meirelles, Flávio Vieira; Trentin, Andréa Gonçalves; Ambrósio, Carlos Eduardo

doi:10.2147/sccaa.s175749

Cited by 12 publications

(17 citation statements)

References 54 publications

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“…From a morphological point of view, these cells show a fibroblast-like shape, which changes during the culture from round to spindle-shaped. Similar cell morphology has been confirmed by many studies not only in rabbits [ 38 , 39 ] but also in other animal species, such as dogs [ 40 ], pigs [ 41 ], horse [ 42 , 43 ] and humans [ 44 , 45 ]. To monitor the rate of proliferation, we evaluated the doubling time of the cell population (PDT).…”

Section: Discussionsupporting

confidence: 81%

“…In general, these cells are defined as CD29 + , CD44 + , CD73 + , CD90 + , CD105 + , CD34 − and CD45 − . A similar phenotype, even though with small differences, was confirmed also for rabbit AT-MSCs [ 39 , 50 ]. The positivity for CD49f marker (integrin α6), which is associated with cell pluripotency, is described by the present study in accordance with previous reports of [ 51 , 52 , 53 ], who claimed the expression of CD49f in MSCs from various sources.…”

Section: Discussionsupporting

confidence: 58%

“…The positive expression of surface and intracellular markers was confirmed by confocal microscopy in both rAT-MSCs and hAT-MSCs. Studies comparing the phenotype of human and rabbit AT-MSCs [ 39 , 50 , 56 ] point out discrepancies in the expression of CD73, CD90 and CD105. Different clones of antibodies for these markers were tested on various rMSCs to distinguish the most appropriate antibodies for rabbit species.…”

Section: Discussionmentioning

confidence: 99%

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Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells

Tirpáková

Vašíček

Svoradová

et al. 2021

Genes

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Although the rabbit is a frequently used biological model, the phenotype of rabbit adipose-derived mesenchymal stem cells (rAT-MSCs) is not well characterized. One of the reasons is the absence of specific anti-rabbit antibodies. The study aimed to characterize rAT-MSCs using flow cytometry and PCR methods, especially digital droplet PCR, which confirmed the expression of selected markers at the mRNA level. A combination of these methods validated the expression of MSCs markers (CD29, CD44, CD73, CD90 and CD105). In addition, cells were also positive for CD49f, vimentin, desmin, α-SMA, ALDH and also for the pluripotent markers: NANOG, OCT4 and SOX2. Moreover, the present study proved the ability of rAT-MSCs to differentiate into a neurogenic lineage based on the confirmed expression of neuronal markers ENO2 and MAP2. Obtained results suggest that rAT-MSCs have, despite the slight differences in marker expression, the similar phenotype as human AT-MSCs and possess the neurodifferentiation ability. Accordingly, rAT-MSCs should be subjected to further studies with potential application in veterinary medicine but also, in case of their cryopreservation, as a source of genetic information of endangered species stored in the gene bank.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

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Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells

Tirpáková

Vašíček

Svoradová

et al. 2021

Genes

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“…Though mice are still the most studied and used model for basic and applied research, it has considerable limitations, in special, in early development, and in overall physiology when compared to humans [21]. Rabbits are also considered adequate models for translational research [22,23]; however, its in vitro cell culture and pluripotency acquisition still lack further robust characterization [24]. In this context, domestic animal species such as swine, small ruminants, and even companion animals such as dogs may provide an important contribution to regenerative and translational medicine.…”

Section: Introductionmentioning

confidence: 99%

Generation of induced pluripotent stem cells from large domestic animals

et al. 2020

Self Cite

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Background: Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results. Methods: Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation. Results: Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs). Conclusions: The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models.

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“…BM-MSC-adherent cells were strongly positive for CD90, whereas Zomer et al. [27] observed that CD90 expression was lower in rabbit MSCs when compared with human MSCs. In addition, the majority of BM-MSC-adherent cells showed no expression for CD34, which is one of the most important characteristics of MSCs [28], [29].…”

Section: Discussionmentioning

confidence: 97%

In vitro periodontal ligament cell expansion by co-culture method and formation of multi-layered periodontal ligament-derived cell sheets

Safi

Hussein

Al-Shammari

2019

Regenerative Therapy

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ObjectivePer-implantitis is one of the implant treatment complications. Dentists have failed to restore damaged periodontium by using conventional therapies. Tissue engineering (stem cells, scaffold and growth factors) aims to reconstruct natural tissues. The paper aimed to isolate both periodontal ligament stem cells (PDLSCs) and bone marrow mesenchymal stem cells (BMMSCs) and use them in a co-culture method to create three-layered cell sheets for reconstructing natural periodontal ligament (PDL) tissue.Materials and methodsBMMSCs were isolated from rabbit tibia and femur, and PDLSC culture was established from the lower right incisor. The cells were co-cultured to induce BMMSC differentiation into PDL cells. Cell morphology, stem cells and PDL-specific markers (CD90, CD34, and periostin) were also detected using immunofluorescent assay. Co-cultured cell monolayers were detached using temperature-responsive tissue culture dishes and collagen graft to create the three-layer construct. The 3D-engineered tissue was examined histologically and by field emission scanning electron microscopy (FESEM).ResultsBMMSCs co-cultured with PDLSCs successfully induced more PDL cells. The newly induced PDL cells exhibited periostin and CD90 expression. Fluorescence green intensity was measured for the co-cultured cells that were stained with periostin, the mean fluorescence green intensity (periostin expression) was significantly higher for the newly induced PDL cells after 1, 2, and 3 weeks when compared with control (BM-MSCs), at 21 days non-significant difference was measured when compared with control (PDLSCs).The results showed the successful formation of 3D multilayer PDL tissue. Histological cross-section showed cell sheets and the stable adhesion between them. FESEM examination was conducted for the cross-section, showing three-layered cell sheets with stable adhesion between cells.ConclusionsThe results of this paper report that the three layered-cell sheets were successfully constructed by the novel use of collagen graft as a scaffold to be used in treatment of periodontitis and to envelop the dental implants to create biohybrid implant.

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Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research

Cited by 12 publications

References 54 publications

Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells

Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells

Generation of induced pluripotent stem cells from large domestic animals

In vitro periodontal ligament cell expansion by co-culture method and formation of multi-layered periodontal ligament-derived cell sheets

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