Distinct granuloma responses in C57BL/6J and BALB/cByJ mice in response to pristane

Abstract

Summary Granuloma formation is an inflammatory response of the host against invading pathogens or indigestible substances. We generated mesenteric oil granulomas by injecting pristane into the peritoneal cavity of mice, and oil granuloma formation in C57BL/6J and BALB/cByJ mice was compared. The formation and kinetics of oil granulomas were distinct between the two strains. In C57BL/6J mice, injected pristane induced oil granuloma formation at both the mesenteric centers (MG) and margins (SG). MG was resolving by 11 weeks, whereas SG persisted. In BALB/cByJ mice, MG developed slower but persisted longer than in C57BL/6J mice, whereas SG resolved sooner than in C57BL/6J mice. Injection of India ink revealed that phagocytes were mainly localized to the SG in C57BL/6J mice, but were diffusely located in both MG and SG of BALB/cByJ mice. SG cells expressed more monocyte chemotactic protein-1 mRNA than MG cells in C57BL/6J mice, but there was no difference in MCP-1 expression between the MG and SG in BALB/cByJ mice. These observations suggest that the recruitment of inflammatory leukocytes under the direction of chemokines differentiates the patterns of granuloma responses to pristane in C57BL/6J and BALB/cByJ mice.

Emerging Trends in the Formation and Function of Tuberculosis Granulomas

“…Not only B-cells are present in granuloma but also they could be important for their maturation. This is suggested in pristane induced oil granuloma formation (Chen et al, 2010) and during Schistosoma japonicum infection (Ji et al, 2008) where the absence of B-cells results in a marked delay in granuloma formation. In the context of the TB, although granulomas form in the absence of B-cells, their numbers and size remain lower and they hardly become inflammatory (Bosio et al, 2000; Maglione et al, 2007).…”

Efficient role of IgH 3′ regulatory region deficient B-cells in the development of oil granulomas

“…Pristine-induced oil granuloma generation is different with respect to mouse substrains [24]. Before assessing the influence of an IgH 3′RR-deleted allele vs a wt allele in B-cell recruitment in oil granulomas we firstly investigated heterozygous IgH a Δ3′RR /b wt mice.…”

“…Serosal granulomas (SG) are located at the interface of the mesenteric margins and gut. Mesenteric granulomas (MG) are located around the center of the mesenteric tissue [23, 24]. Oil granulomas are considered as tertiary lymphoid tissues constituted of monocytes, granulocytes, T-cells and B-cells.…”

Genetic regulation of pristane‐induced oil granuloma responses

“… mesenteric granuloma (MG) that locate in the mesenteric centers away from fat and blood vessels; and serosal granuloma (SG) that appear individually at mesenteric margins (Chen et al. 2010).…”

“…Histologically, MG and SG exhibit similar populations of inflammatory leucocytes, that include lymphocytes, dendritic cells, neutrophils and macrophages (Chen et al. , 2010).…”