Distinct Gut Microbiota and Metabolite Profiles Induced by Different Feeding Methods in Healthy Chinese Infants

Li, Na; Yan, Fang; Wang, Nana; Song, Yue; Yue, Yingxue; Guan, Jiaqi; Li, Bailiang; Gui-cheng, Huo

doi:10.3389/fmicb.2020.00714

Cited by 43 publications

(61 citation statements)

References 68 publications

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“… 16 However, substantial differences have been found between the gut microbiota composition of formula-fed and breastfed infants. 16 , 35 Those differences are due in part to the low concentrations and different structures of oligosaccharides found in infant formulas compared to human milk. 15 Infant formulas are based in bovine milk, which does not contain type-1 oligosaccharides, 15 lacking therefore the predominant LNB structural unit of HMOs.…”

Section: Discussionmentioning

confidence: 99%

Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice

et al. 2021

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Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development. A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto- N -biose (LNB) and galacto- N -biose on the fecal microbiota and host–microbiota interactions. 16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased ( Ruminococcus and Oscillospira ) or decreased ( Eubacterium and Clostridium ) in all disaccharide-supplemented groups. Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides. An increment of the relative abundance of Coprococcus genus was only observed with 3FN. As well, LNB significantly increased the relative abundance of Bifidobacterium , whereas the absolute levels of this genus, as measured by quantitative real-time PCR, did not significantly increase. OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation. The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol. 3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue. The present study revealed that the four disaccharides show efficacy in producing beneficial compositional shifts of the gut microbiota and in addition, the 3FN demonstrated physiological and immunomodulatory roles.

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Section: Discussionmentioning

confidence: 99%

Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice

et al. 2021

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“…In the mixed-fed group they detected increased levels of cis-11,14-eicosadienoic acid, stearidonic acid, capric acid, myristic acid, docosahexaenoic acid, cis-8,11,14-eicosatrienoic acid, and 15-oxoete. Interestingly, only eicosapentaenoic acid was elevated in stool samples of the exclusive formula-fed group (Li et al, 2020). Varying results of fecal fatty acid profiles between different studies could be due to the variable fatty acid composition of different infant formulas.…”

Section: Increased Fatty Acids In Breastfed Children Reflected Fatty Acid Composition Of Breast Milkmentioning

confidence: 93%

“…On the contrary, a different study reported myristic acid to be significantly higher in formula-fed infants (Chow et al, 2014). Li et al observed a series of different fatty acids, significantly altered in fecal samples of breastfed, mixed-fed, exclusively formula-fed or complementary food-fed infants (Li et al, 2020). In the mixed-fed group they detected increased levels of cis-11,14-eicosadienoic acid, stearidonic acid, capric acid, myristic acid, docosahexaenoic acid, cis-8,11,14-eicosatrienoic acid, and 15-oxoete.…”

Section: Increased Fatty Acids In Breastfed Children Reflected Fatty Acid Composition Of Breast Milkmentioning

confidence: 95%

“…Several studies consistently showed that children fed with breast milk or formula have a clear and distinct fecal metabolome, as recently summarized by Laurens et al (Laurens et al, 2020). Targeted or non-targeted profiling of infant stool resulted in the detection of various metabolite classes including short chain fatty acids (SCFAs), amino acids, amino acid catabolites, fatty acids, human milk oligosaccharides, monosaccharides, purine degradation products or even plantbased metabolites from soy-based formula (Chow et al, 2014;Martin et al, 2014;Dotz et al, 2015;Dotz et al, 2016;Martin et al, 2016b;Bridgman et al, 2017;He et al, 2019;Brink et al, 2020;Kok et al, 2020;Li et al, 2020).…”

Section: Time Independent Feeding Effects On the Fecal Metabolomementioning

confidence: 99%

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Longitudinal Profiles of Dietary and Microbial Metabolites in Formula- and Breastfed Infants

Sillner

Walker

Lucio

et al. 2021

Front. Mol. Biosci.

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The early-life metabolome of the intestinal tract is dynamically influenced by colonization of gut microbiota which in turn is affected by nutrition, i.e. breast milk or formula. A detailed examination of fecal metabolites was performed to investigate the effect of probiotics in formula compared to control formula and breast milk within the first months of life in healthy neonates. A broad metabolomics approach was conceptualized to describe fecal polar and semi-polar metabolites affected by feeding type within the first year of life. Fecal metabolomes were clearly distinct between formula- and breastfed infants, mainly originating from diet and microbial metabolism. Unsaturated fatty acids and human milk oligosaccharides were increased in breastfed, whereas Maillard products were found in feces of formula-fed children. Altered microbial metabolism was represented by bile acids and aromatic amino acid metabolites. Elevated levels of sulfated bile acids were detected in stool samples of breastfed infants, whereas secondary bile acids were increased in formula-fed infants. Microbial co-metabolism was supported by significant correlation between chenodeoxycholic or lithocholic acid and members of Clostridia. Fecal metabolites showed strong inter- and intra-individual behavior with features uniquely present in certain infants and at specific time points. Nevertheless, metabolite profiles converged at the end of the first year, coinciding with solid food introduction.

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“…Purified amplicons were pooled in equimolar and paired-end sequenced (2 × 250) on an Illumina platform. The details of DNA extraction, PCR amplification, quantitative PCR, amplicons purification, sequencing, and high-quality sequences criteria are those described by Li ( Li et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Distinct Effects of Milks From Various Animal Types on Infant Fecal Microbiota Through in vitro Fermentations

Guan

et al. 2020

Front. Microbiol.

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Human milk is compatible with infant intestinal microbiota and is vital for infant health. However, most infants do not receive sufficient exclusive breastfeeding, and the effects of including other types of animal milk on the gut microbiota of infants are unclear. Therefore, the objective of this study was to elucidate the impact of milk from various animal sources on infant fecal microbiota through in vitro fermentation. The types of milk assessed include cow milk, goat milk, camel milk, mare milk, human milk, and infant formula milk. Here we determined the gas pressure, pH, and microbiota after 24 h fermentation. Results showed that mare milk had the lowest gas pressure rating, with levels similar to human milk. More so, pH analysis demonstrated that other milk types were identical to human milk. Bacterial 16S rRNA gene sequence analysis revealed that all milk types increased the abundance of Bifidobacterium and Lactobacillus , which was proportional to the lactose content of milk. Moreover, mare milk also significantly increased the relative abundance of Akkermansia . Collectively, results from mare milk (gas pressure, pH, and microbiota) were comparable to that of human milk, and thus support the theoretical basis for exploring the development of a mare milk-based infant formula.

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Distinct Gut Microbiota and Metabolite Profiles Induced by Different Feeding Methods in Healthy Chinese Infants

Cited by 43 publications

References 68 publications

Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice

Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice

Longitudinal Profiles of Dietary and Microbial Metabolites in Formula- and Breastfed Infants

Distinct Effects of Milks From Various Animal Types on Infant Fecal Microbiota Through in vitro Fermentations

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