Distinct H3K9me3 and DNA methylation modifications during mouse spermatogenesis

Liu, Yingdong; Zhang, Yanping; Yin, Jie; Gao, Yawei; Li, Yanhe; Bai, Dandan; He, Weiming; Li, Xueliang; Zhang, Pengfei; Li, Rongnan; Zhang, Lingkai; Jia, Yanping; Lin, Jiamao; Zheng, Yi; Wang, Hong; Gao, Shaorong; Liu, Wenqiang

doi:10.1074/jbc.ra119.010496

Cited by 43 publications

(20 citation statements)

References 41 publications

(47 reference statements)

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“…The purified DNA was analyzed by RT-qPCR. The primer was designed by published H3K9me3 ChIP-seq data in mouse undifferentiated spermatogonia cells ( Liu et al, 2019 ). Then, ChIP-qPCR primers were designed around the transcription initiation site, and the size of the product was about 200 bp ( Asp, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase

Chen

Liu

et al. 2020

Front. Genet.

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SETDB1, a histone H3 lysine 9 (H3K9) methyltransferase, is crucial in meiosis and embryo development. This study aimed to investigate whether SETDB1 was associated with spermatogonial stem cells (SSC) homeostasis. We found that knockdown of Setdb1 impaired cell proliferation, led to an increase in reactive oxygen species (ROS) level through NADPH oxidase, and Setdb1 deficiency activated ROS downstream signaling pathways, including JNK and p38 MAPK, which possibly contributed to SSC apoptosis. Melatonin scavenged ROS and rescued the phenotype of Setdb1 KD. In addition, we demonstrated that SETDB1 regulated NADPH oxidase 4 ( Nox4 ) and E2F1 . Therefore, this study uncovers the new roles of SETDB1 in mediating intracellular ROS homeostasis for the survival of SSC.

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Section: Methodsmentioning

confidence: 99%

The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase

Chen

Liu

et al. 2020

Front. Genet.

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“… 78 DNA methylation occurs in the covalent modification of cytosine 5-C, mainly in the context of cytosine guanine dinucleotide. 79 The mechanism for establishing and maintaining DNA methylation is well established and involves 3 mammalian methyltransferases (Dnmts): Dnmt1, 3A, and 3B. 80 Germ-line deletion studies in mice have identified an essential role for Dnmts in embryonic heart development.…”

Section: Epigenetic Regulation Of Ror Receptor Pathwaymentioning

confidence: 99%

Roles of Wnt Signaling Pathway and ROR2 Receptor in Embryonic Development: An Update Review Article

Guo

Xing

2022

Genet Epigenet

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The Wnt family is a large class of highly conserved cysteine-rich secretory glycoproteins that play a vital role in various cellular and physiological courses through different signaling pathways during embryogenesis and tissue homeostasis 3. Wnt5a is a secreted glycoprotein that belongs to the noncanonical Wnt family and is involved in a wide range of developmental and tissue homeostasis. A growing body of evidence suggests that Wnt5a affects embryonic development, signaling through various receptors, starting with the activation of β-catenin by Wnt5a. In addition to affecting planar cell polarity and Ca2+ pathways, β-catenin also includes multiple signaling cascades that regulate various cell functions. Secondly, Wnt5a can bind to Ror receptors to mediate noncanonical Wnt signaling and a significant ligand for Ror2 in vertebrates. Consistent with the multiple functions of Wnt5A/Ror2 signaling, Wnt5A knockout mice exhibited various phenotypic defects, including an inability to extend the anterior and posterior axes of the embryo. Numerous essential roles of Wnt5a/Ror2 in development have been demonstrated. Therefore, Ror signaling pathway become a necessary target for diagnosing and treating human diseases. The Wnt5a- Ror2 signaling pathway as a critical factor has attracted extensive attention.

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“…In contrast to somatic cells, transcriptional activity does not always occur during gametogenesis. In male mice, transcriptional silencing occurs during spermatocyte meiotic entry and again during spermatid elongation and maturation, which involves histone exchange and altered chromatin compaction [53,54] (Figure 3A). The mRNAs are transcribed during male germ cell differentiation and deposited with long poly(A) tails, keeping them in a repressed state (Figure 3A).…”

Section: Pabpcs In Spermatogenesismentioning

confidence: 99%

Revisiting poly(A)‐binding proteins: Multifaceted regulators during gametogenesis and early embryogenesis

Zhao

Fan

2021

BioEssays

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Post-transcriptional regulation faces a distinctive challenge in gametes. Transcription is limited when the germ cells enter the division phase due to condensed chromatin, while gene expression during gamete maturation, fertilization, and early cleavage depends on existing mRNA post-transcriptional coordination. The dynamics of the 3ʹ-poly(A) tail play crucial roles in defining mRNA fate. The 3ʹ-poly(A) tail is covered with poly(A)-binding proteins (PABPs) that help to mediate mRNA metabolism and recent work has shed light on the number and function of germ cell-specific expressed PABPs. There are two structurally different PABP groups distinguished by their cytoplasmic and nuclear localization. Both lack catalytic activity but are coupled with various roles through their interaction with multifunctional partners during mRNA metabolism. Here, we present a synopsis of PABP function during gametogenesis and early embryogenesis and describe both conventional and current models of the functions and regulation of PABPs, with an emphasis on the physiological significance of how germ cell-specific PABPs potentially affect human fertility.

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Distinct H3K9me3 and DNA methylation modifications during mouse spermatogenesis

Cited by 43 publications

References 41 publications

The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase

The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase

Roles of Wnt Signaling Pathway and ROR2 Receptor in Embryonic Development: An Update Review Article

Revisiting poly(A)‐binding proteins: Multifaceted regulators during gametogenesis and early embryogenesis

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