Distinct levels of genetic diversity of Borrelia burgdorferi are associated with different aspects of pathogenicity

Baranton, Guy; Seinost, Gerald; Theodore, George H.; Postić, D.; Dykhuizen, Daniel E.

doi:10.1016/s0923-2508(01)01186-x

Cited by 92 publications

(79 citation statements)

References 22 publications

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“…In accordance with other investigators using animal models (29), it is possible that ospC diversity is less important as a predictor of mammalian and human invasion than proposed previously (3,25). Thus, given the extensive diversity of B. burgdorferi ospC types that may infect humans, alternative genetic markers should be investigated that may better correlate with and explain B. burgdorferi disseminated disease.…”

Section: Discussionsupporting

confidence: 90%

Borrelia burgdorferi ospC Heterogeneity among Human and Murine Isolates from a Defined Region of Northern Maryland and Southern Pennsylvania: Lack of Correlation with Invasive and Noninvasive Genotypes

et al. 2005

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B. burgdorferi invasiveness correlates with ospC genotype. To test this hypothesis and whether identical genotypes infect humans and small mammals in specific sites, B. burgdorferi ospC heterogeneity was tested among isolates from northern Maryland and southern Pennsylvania. Six culture-positive patients allowed collection of small animals from their properties, and spirochetes from animals trapped within 300 yards of each patient's home were isolated. 3 ospC sequences were compared to reference sequences. Of the 7 human and 15 mouse DNA templates that produced reliable sequences, all clustered with references into only four and seven distinct clades, respectively. A human and a mouse isolate with the same ospC were seen in only one locality, and five of six sites contained two or more B. burgdorferi ospC clones. Four invasive patient isolates and six small mammal isolates clustered with "noninvasive" reference ospC genotypes. A high degree of ospC diversity exists among B. burgdorferi isolates in Maryland and Pennsylvania, even in narrowly defined geographic localities. Dissemination in mice and humans by noninvasive ospC types contradicts the ospC invasiveness hypothesis. Alternative genetic markers for B. burgdorferi disseminated disease should be investigated.

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Section: Discussionsupporting

confidence: 90%

Borrelia burgdorferi ospC Heterogeneity among Human and Murine Isolates from a Defined Region of Northern Maryland and Southern Pennsylvania: Lack of Correlation with Invasive and Noninvasive Genotypes

et al. 2005

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“…at 33°C in 5% CO 2 . The Escherichia coli host cell strains used for cloning and expression of recombinant proteins were INF␣F (Invitrogen, Leek, The Netherlands) and M15 (Qiagen, Hilden, Germany), respectively.…”

Section: Methodsmentioning

confidence: 99%

Species-SpecificSerodiagnosis of Lyme Arthritis and Neuroborreliosis Due to Borreliaburgdorferi Sensu Stricto, B. afzelii , and B.garinii by Using Decorin Binding ProteinA

Heikkilä

Seppälä

Saxén³

et al. 2002

J Clin Microbiol

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The antigenic potential of decorin binding protein A (DbpA) was evaluated in serodiagnosis of human Lyme borreliosis (LB). The dbpA was cloned and sequenced from the three pathogenic Borrelia species common in Europe. Sequence analysis revealed high interspecies heterogeneity. The identity of the predicted amino acid sequences was 43 to 62% among Borrelia burgdorferi sensu stricto, B. afzelii, and B. garinii. The respective recombinant DbpAs (rDbpAs) were produced and tested as antigens by Western blotting and enzyme-linked immunosorbent assay (ELISA). One hundred percent of patients with neuroborreliosis (NB) and 93% of patients with Lyme arthritis (LA) reacted positively. Sera from the majority of patients reacted with one rDbpA only and had no or low cross-reactivity to other two variant proteins. In patients with culture-positive erythema migrans (EM), the sensitivity of rDbpA immunoglobulin G (IgG) or IgM ELISA was low. The DbpA seems to be a sensitive and specific antigen for the serodiagnosis of LA or NB, but not of EM, provided that variants from all three pathogenic borrelial species are included in the combined set of antigens.

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“…Molecular analyses such as protein and plasmid profiles, reactivity with monoclonal antibodies, DNA-DNA relatedness, 16 rRNA, flagellin and ospA gene sequence analyses, and finally multilocus enzyme electrophoresis demonstrated that these B. burgdorferi isolates are phenotypically and genotypically divergent (11,12,28,35,38,40). These studies also established that all isolates are gathered in several species and genomic groups that include B. burgdorferi sensu stricto, B. garinii, B. afzelii, B. japonica, B. andersonii, B. valaisiana, B. lusitaniae, B. tanukii, B. turdi, B. bissettii, and B. sinica species (3,13,21,26,33). The term B. burgdorferi sensu lato is generally used to refer to all isolates belonging to these species or genomic groups.…”

mentioning

confidence: 93%

PCR-Reverse Line Blot Typing Method Underscores the Genomic Heterogeneity of Borrelia valaisiana Species and Suggests Its Potential Involvement in Lyme Disease

Godfroid

Humair

et al. 2003

J Clin Microbiol

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Detection of the Borrelia burgdorferi sensu lato complex in biological samples is currently done by conventional immunological and molecular biological methods. To improve on the accuracy of these methods and to simplify the procedure for testing large numbers of samples, a solid-phase sandwich hybridization system readily applicable to the detection of PCR products has been designed. This colorimetric detection system relies on the use of polybiotinylated detection probes and of specific capture oligonucleotides covalently linked at allocated positions on nylon membrane strips. From a phylogenetic analysis on a great number of ospA gene sequences, we have designed and synthesized a set of PCR primers specific to the five Borrelia burgdorferi sensu lato genospecies present in Europe and a subset of probes (capture and detection probes) specific to these five genospecies (B. burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, and B. lusitaniae). This combined PCR hybridization system was evaluated with a large number of various B. burgdorferi isolates and clinical specimens. These analyses clearly showed that the system could be used as a typing method to distinguish five genospecies belonging to the B. burgdorferi sensu lato complex. In addition, the study showed that B. valaisiana strains might be more heterologous than suspected up to now and clustered into three genomic groups.

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Distinct levels of genetic diversity of Borrelia burgdorferi are associated with different aspects of pathogenicity

Cited by 92 publications

References 22 publications

Borrelia burgdorferi ospC Heterogeneity among Human and Murine Isolates from a Defined Region of Northern Maryland and Southern Pennsylvania: Lack of Correlation with Invasive and Noninvasive Genotypes

Borrelia burgdorferi ospC Heterogeneity among Human and Murine Isolates from a Defined Region of Northern Maryland and Southern Pennsylvania: Lack of Correlation with Invasive and Noninvasive Genotypes

Species-SpecificSerodiagnosis of Lyme Arthritis and Neuroborreliosis Due to Borreliaburgdorferi Sensu Stricto, B. afzelii , and B.garinii by Using Decorin Binding ProteinA

PCR-Reverse Line Blot Typing Method Underscores the Genomic Heterogeneity of Borrelia valaisiana Species and Suggests Its Potential Involvement in Lyme Disease

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