“…These powerful methods provide unique insights into health and disease in contrast to bulk transcriptomic and classical histological analyses. Single-cell RNA-sequencing and spatial transcriptomic approaches have shown that glia isolated from healthy and disease-associated brain regions respond across broad cellular states for microglia (Tay et al, 2018;Hammond et al, 2019;Jordão et al, 2019;Li et al, 2019;Masuda et al, 2019), astrocytes (Cahoy et al, 2008;Zamanian et al, 2012;Boisvert et al, 2018;Bradley et al, 2019;Batiuk et al, 2020;Bayraktar et al, 2020;Das et al, 2020), and oligodendrocytes (Jäkel et al, 2019;Spitzer et al, 2019;Floriddia et al, 2020). Advances in single-cell proteomics have also enabled the highthroughput investigation of key biological questions involving protein binding, modifications, and degradation, that cannot be assessed at the transcriptomic level (Slavov, 2020).…”