Distinct patterns of expression of keratinocyte growth factor and its receptor in endometrial carcinoma

Abstract

factor (FGF) family. KGF is a stromally derived, paracrine growth factor specifically mitogenic for a variety of epithelial cells. The KGF receptor (KGFR), which is a splice ples were obtained at the primary surgery before any treatment was initiated. In addition, endometrial tissues from 19 premenopausal women with normal menstrual cycles were examined. The expression of specific mRNAs in the endometrial samples was assessed using quantitative reverse transcriptase polymerase chain reaction. The results were analyzed by the nonparametric Kruskal-Wallis statistic. RESULTS.The KGF mRNA expression was significantly lower in endometrial adenocarcinoma tissue compared with cycling endometrial tissues, whereas no difference was found between carcinosarcoma tissue and cycling endometrium. The relative level of KGFR mRNA in endometrial adenocarcinoma did not differ from that in cycling endometrium, but was significantly higher compared with carcinosarcomas. No differences were observed in FGFR-2 mRNA expression between cycling endometrium and tumor tissues. CONCLUSIONS.To the authors' knowledge, this study demonstrates for the first time the expression of KGF, KGFR, and FGFR-2 mRNAs in endometrial adenocarcinoma and in carcinosarcoma tissues. The relative level of KGF mRNA expression in adenocarcinoma tissue is decreased compared with that in cycling endometrium. The change in epithelial/stromal cell prominence between cycling endometrium and adenocarcinoma tissue may account for the difference in KGF expression but does not explain why KGF receptor expression in same tissues remains unchanged. of such products.

Immunohistochemical analysis of keratinocyte growth factor and fibroblast growth factor 10 expression in psoriasis

“…Our immunohistochemical study also revealed that, in agreement with previous observations in normal epidermal tissues (15,23), KGFR protein appeared distributed over all the epidermal layers, with a preferential localization on the suprabasal spinous layers, in the skin samples analyzed either from normal or psoriatic patients. However, no significant differences in KGFR expression were revealed by quantitative analysis of the immunostaining (data not shown), and several reports demonstrate variability in the expression of KGFR at the protein level in pathological tissues (24,25) including psoriasis (19), as a consequence of the stage of the disease and growth factor stimulation. In fact, it has been suggested that modulation of KGF expression may induce ligand‐dependent KGFR downregulation and consequent modulation in the receptor expression at the protein level (15).…”

Activation of Mitogen-Activated Protein Kinase Pathway by Keratinocyte Growth Factor or Fibroblast Growth Factor-10 Promotes Cell Proliferation in Human Endometrial Carcinoma Cells

“…Although Pekonen et al (26) failed to show KGF expression in human endometrial adenocarcinoma cells, in our study we detected the presence of KGF transcripts in endometrial cancer cell lines, such as HEC-1 and Ishikawa cells. Siegfried et al (27) suggested that KGF mRNA expression was significantly lower in endometrial adenocarcinoma tissue, compared with cycling endometrial tissue. However, as shown in Fig.…”

Effects of Keratinocyte Growth Factor in the Endometrium of Rhesus Macaques during the Luteal-Follicular Transition1

“…The KGFR has been detected in human endometrium (10,17), human endometrial carcinoma cells (23), and macaque endometrium (24). Human endometrial KGFR messenger ribonucleic acid (mRNA) is reported to be increased by estrogen (17) and the relative levels of KGFR mRNA in endometrial adenocarcinoma are reported to be similar to those in cycling endometrium (23).…”