Distinct polymer extraction and cellulose DP reduction for complete cellulose hydrolysis under mild chemical pretreatments in sugarcane

Hu, Meng; Yu, Hua; Yu, Li; Li, Ao; Cai, Qiuming; Liu, Peng; Tu, Yuanyuan; Wang, Yanting; Hu, Ruofei; Hu, Bo; Peng, Liangcai; Xia, Tao

doi:10.1016/j.carbpol.2018.08.039

Cited by 53 publications

(17 citation statements)

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“…It has been demonstrated that lignocellulose features significantly affect biomass enzymatic saccharification under various physical and chemical pretreatments [21][22][23][24][25][26][27][28][29]. Due to markedly increased cellulose levels in PtoDET2-OE lines, we examined cellulose crystalline index (CrI) and degree of polymerization (DP), which have been examined as major cellulose features negatively affecting biomass enzymatic saccharification.…”

Section: Altered Lignocellulose Features In Transgenic Poplar Plantsmentioning

confidence: 99%

“…For cellulosic ethanol production, initial biomass pretreatments are considered as crucial step for enhancing sequential enzymatic hydrolysis and final yeast fermentation [1,[21][22][23]. Over the past years, various chemical pretreatments have been performed to reduce lignocellulose recalcitrance in grasses, such as H 2 SO 4 , NaOH, CaO, Na 2 S + Na 2 CO 3 [24][25][26][27][28][29]. Acids and alkalis (H 2 SO 4 and NaOH) are the classical agents applied for pretreatment to enhance biomass enzymatic hydrolysis in woody plants, but these methods release wastes and cause serious secondary environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

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Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar

Fan

Qin

et al. 2020

Biotechnol Biofuels

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Background: As a leading biomass feedstock, poplar plants provide enormous lignocellulose resource convertible for biofuels and bio-chemicals. However, lignocellulose recalcitrance particularly in wood plants, basically causes a costly bioethanol production unacceptable for commercial marketing with potential secondary pollution to the environment. Therefore, it becomes important to reduce lignocellulose recalcitrance by genetic modification of plant cell walls, and meanwhile to establish advanced biomass process technology in woody plants. Brassinosteroids, plantspecific steroid hormones, are considered to participate in plant growth and development for biomass production, but little has been reported about brassinosteroids roles in plant cell wall assembly and modification. In this study, we generated transgenic poplar plant that overexpressed DEETIOLATED2 gene for brassinosteroids overproduction. We then detected cell wall feature alteration and examined biomass enzymatic saccharification for bioethanol production under various chemical pretreatments.Results: Compared with wild type, the PtoDET2 overexpressed transgenic plants contained much higher brassinosteroids levels. The transgenic poplar also exhibited significantly enhanced plant growth rate and biomass yield by increasing xylem development and cell wall polymer deposition. Meanwhile, the transgenic plants showed significantly improved lignocellulose features such as reduced cellulose crystalline index and degree of polymerization values and decreased hemicellulose xylose/arabinose ratio for raised biomass porosity and accessibility, which led to integrated enhancement on biomass enzymatic saccharification and bioethanol yield under various chemical pretreatments. In contrast, the CRISPR/Cas9-generated mutation of PtoDET2 showed significantly lower brassinosteroids level for reduced biomass saccharification and bioethanol yield, compared to the wild type. Notably, the optimal green-like pretreatment could even achieve the highest bioethanol yield by effective lignin extraction in the transgenic plant. Hence, this study proposed a mechanistic model elucidating how brassinosteroid regulates cell © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article' Full list of author information is available at the end of the article wall modification for reduced lignocellulose recalcitrance and increased biomass porosity and accessibility for high bioethanol production. Conclusions:This study has demonstrated a powerful strategy to enhance cell...

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Section: Altered Lignocellulose Features In Transgenic Poplar Plantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar

Fan

Qin

et al. 2020

Biotechnol Biofuels

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“…Therefore, it is possible that other factors are involved in determining saccharification efficiency. In fact, two major characteristics of cell wall—the cellulose crystalline index (CrI) and the degree of polymerization (DP) of β-1,4-glucans—have been considered essential to negatively affect biomass digestibility under various pretreatments in different species, including sugarcane [25, 26, 49, 50]. In addition, recent studies have suggested that the arabinose (Ara) substitution degree of xylans could reduce cellulose crystallinity for positively affecting biomass enzymatic digestibility under chemical pretreatments in sugarcane and other grasses [25, 51, 52].…”

Section: Discussionmentioning

confidence: 99%

“…This is mainly achieved by ( i ) using biomass feedstocks that possess less cell-wall recalcitrance; ( ii ) using suitable pretreatments of the raw material for hemicellulose sugars extraction; and ( iii ) enzymatic treatment for the cellulose conversion to glucose that will be converted to ethanol by fermentation [23]. Among the pretreatment technologies, which include Organosolv treatment [24], acid and alkali pretreatments [25, 26], and steam explosion [27], the Organosolv process has been considered as one of the most promising for cellulosic ethanol production [23, 24, 28, 29]. This type of pretreatment involves the use of an organic liquid and water to partially hydrolyze lignin bonds and lignin-carbohydrate bonds, resulting in a solid residue consisting of mainly cellulose and some hemicellulose [30, 31].…”

Section: Introductionmentioning

confidence: 99%

Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility

Souza

Pacheco

Duarte

et al. 2019

Biotechnol Biofuels

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Background Sugarcane ( Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results We identified six BAHD genes in the sugarcane genome (Sac BAHD s) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Electronic supplementary material The online version of this article (10.1186/s13068-019-1450-7) contains supplementary material, which is available to authorized users.

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“…The structure is similar to composites in that celluloses of stiff fillers are surrounded by lignin and hemi-cellulose, which acted as cements [9,10]. Cellulose of semi-crystalline is the main component of such non-wood and wood structures, and provides outstanding mechanical and thermal properties due to strong hydrogen bonding between hydroxyl groups in the linear polymer of β -(1→4)- d -glucopyranose units [11,12], whereas the lignin and hemi-cellulose components are amorphous [13]. Thus, cellulose is applied to plastic substitute materials or various reinforcement composites as organic fillers [14,15,16].…”

Section: Introductionmentioning

confidence: 99%

Electron Beam Irradiation Isolates Cellulose Nanofiber from Korea “Tall Goldenrod” Invasive Alien Plant Pulp

et al. 2019

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This work investigates the possibility of isolating cellulose nanofibers from pulps of tall goldenrod plant, which are invasive plants in Korea, by a convenient method, without strong acids or high-pressure homogenization, using electron beam irradiation (EBI). The obtained cellulose nanofibers were characterized by scanning electron microscopy (SEM), ultraviolet–visible (UV–vis) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and in terms of their mechanical properties. SEM showed that the initially isolated 10-μm-diameter cellulose fibers became more finely separated with increasing EBI dose, and that cellulose fibers treated with 300 kGy of EBI were separated into long cellulose nanofibers of around 160 nm in diameter. In addition, the paper samples prepared from more finely separated fibers generated by using higher doses of EBI had enhanced UV–vis transmittance. Via the XRD analysis, we observed that cellulose I in the EBI-treated cellulose fibers were gradually converted into a different type of cellulose similar to cellulose type II, as the EBI dose increased. Meanwhile, the TGA demonstrated that the finely separated cellulose fibers observed after administering the high EBI dose had lowered thermal stability due to the reduction of cellulose I but higher char yield. In addition, tensile strengths of paper samples increased with decreasing the diameters of their constituent fibers that result from the different EBI doses used in the preparation of the paper pulp.

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Distinct polymer extraction and cellulose DP reduction for complete cellulose hydrolysis under mild chemical pretreatments in sugarcane

Cited by 53 publications

References 53 publications

Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar

Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar

Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility

Electron Beam Irradiation Isolates Cellulose Nanofiber from Korea “Tall Goldenrod” Invasive Alien Plant Pulp

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