Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing

Abbasi, Sepideh; Sinha, Sarthak; Labit, Elodie; Rosin, Nicole L.; Yoon, Grace; Rahmani, Waleed; Jaffer, Arzina; Sharma, Nilesh; Hagner, Andrew; Shah, Prajay; Arora, Rohit; Yoon, Jessica; Islam, Anowara; Uchida, Aya; Chang, Chih Kai; Stratton, Jo Anne; Scott, R. Wilder; Rossi, Fábio; Underhill, T. Michael; Biernaskie, Jeff

doi:10.1016/j.stem.2020.07.008

Cited by 148 publications

(108 citation statements)

References 50 publications

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“…This regenerative phenomenon, also known as wound‐induced hair follicle neogenesis (WIHN) 29,30 is highly desirable, yet normally not achievable clinically and variable across species, being prominent in spiny mice 31,32 and deficient in rats 33 and pigs 34 . Cellular heterogeneity in the WIHN model has been recently studied using scRNA‐seq 35–39 . The major focus on these studies has been to better understand the lineage origin and the transcriptomic states of regeneration‐competent wound fibroblasts, that can become new dermal papilla cells of neogenic hair follicles.…”

Section: Fibroblast Heterogeneitymentioning

confidence: 99%

More than just bricks and mortar: Fibroblasts and ECM in skin health and disease

Plikus

Krieg

2020

Experimental Dermatology

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Section: Fibroblast Heterogeneitymentioning

confidence: 99%

More than just bricks and mortar: Fibroblasts and ECM in skin health and disease

Plikus

Krieg

2020

Experimental Dermatology

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“…To investigate differences between scarring and regenerative wound repair, we performed a scRNA‐seq analysis from fibroblasts of two scarring wounds and two types of wounds that regenerate hair follicles (Figure 1). We utilized previously published data from Abbasi et al, 2020 and Phan et al, 2020 15,16 . scRNA‐seq experiments performed in Phan et al sequenced all of the cells from single‐cell suspensions of scarring and regenerating wounds.…”

Section: Resultsmentioning

confidence: 99%

“…Generation of scRNA‐seq data (GSE108677) is described 15 . In short, cells were isolated from large full‐thickness wounds of Hic1‐tdTomato mice treated with tamoxifen at P4‐5.…”

Section: Methodsmentioning

confidence: 99%

“…These comparisons of regenerating and scarring wounds using scRNA‐seq have mapped diverse populations of fibroblast within the wound beds but have not fully understood how they can differentiate into dermal papilla to support hair follicle reformation. However, two recent publications investigating the differences between scarring and regenerating wounds by scRNA‐seq now provide a foundational data set to investigate the ability to regenerate 15,16 …”

Section: Introductionmentioning

confidence: 99%

“…It has been recently shown that Hypermethylated in cancer (Hic1) transgenic CreERT2 mice can label both papillary and reticular fibroblasts at varying efficiencies 15 . Histological analysis of lineage traced Hic1‐labelled cells revealed high contribution to regenerating dermal papilla in WIHN.…”

Section: Introductionmentioning

confidence: 99%

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Single‐cell transcriptomic analysis of small and large wounds reveals the distinct spatial organization of regenerative fibroblasts

Phan

Sinha

Biernaskie

et al. 2020

Experimental Dermatology

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Wound-induced hair follicle neogenesis (WIHN) has been an important model to study hair follicle regeneration during wound repair. However, the cellular and molecular components of the dermis that make large wounds more regenerative are not fully understood. Here, we compare and contrast recently published scRNA-seq data of small scarring wounds to wounds that regenerate in hope to elucidate the role of fibroblasts lineages in WIHN. Our analysis revealed an over-representation of the newly identified upper wound fibroblasts in regenerative wound conditions, which express the retinoic acid binding protein Crabp1. This regenerative cell type shares a similar gene signature to the murine papillary fibroblast lineage, which are necessary to support hair follicle morphogenesis and homeostasis. RNA velocity analysis comparing scarring and regenerating wounds revealed the divergent trajectories towards upper and lower wound fibroblasts and that the upper populations were closely associated with the specialized dermal papilla. We also provide analyses and explanation reconciling the inconsistency between the histological lineage tracing and the scRNAseq data from recent reports investigating large wounds. Finally, we performed a computational test to map the spatial location of upper wound fibroblasts in large wounds which revealed that upper peripheral fibroblasts might harbour equivalent regenerative competence as those in the centre. Overall, our scRNA-seq reanalysis combining multiple samples suggests that upper wound fibroblasts are required for hair follicle regeneration and that papillary fibroblasts may migrate from the wound periphery to the centre during wound re-epithelialization. Moreover, data from this publication are made available on our searchable web resource: https://skinr egene ration.org/.

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CSMP: A Self‐Assembled Plant Polysaccharide‐Based Hydrofilm for Enhanced Wound Healing

Qiao,

Zhang,

Liu

et al. 2023

Adv Healthcare Materials

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Wound management remains a critical healthcare issue due to the rising incidence of chronic diseases leading to persistent wounds. Traditional dressings have their limitations, such as potential for further damage during changing and suboptimal healing conditions. Recently, hydrogel‐based dressings have gained attention due to their biocompatibility, biodegradability, and ability to fill wounds. Particularly, polysaccharide‐based hydrogels have shown potential in various medical applications. This study focuses on the development of a novel hydrofilm wound dressing produced from a blend of chia seed mucilage (CSM) and polyvinyl alcohol (PVA), termed CSMP. While the individual properties of CSM and PVA are well‐documented, their combined potential in wound management is largely unexplored. CSMP, coupled with sorbitol and glycerin, and cross‐linked using ultraviolet light, results in a flexible, adhesive, and biocompatible hydrofilm demonstrating superior water absorption, moisturizing, and antibacterial properties. This hydrofilm promotes epithelial cell migration, enhanced collagen production, and outperforms existing commercial dressings in animal tests. The innovative CSMP hydrofilm offers a promising, cost‐effective approach for improved wound care, bridging existing gaps in dressing performance and preparation simplicity. Future research can unlock further applications of such polysaccharide‐based hydrofilm dressings.

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Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing

Cited by 148 publications

References 50 publications

More than just bricks and mortar: Fibroblasts and ECM in skin health and disease

More than just bricks and mortar: Fibroblasts and ECM in skin health and disease

Single‐cell transcriptomic analysis of small and large wounds reveals the distinct spatial organization of regenerative fibroblasts

CSMP: A Self‐Assembled Plant Polysaccharide‐Based Hydrofilm for Enhanced Wound Healing

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