2019
DOI: 10.1016/j.fsi.2019.08.002
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Distinct response of immune gene expression in peripheral blood leucocytes modulated by bacterin vaccine candidates in rainbow trout Oncorhynchus mykiss: A potential in vitro screening and batch testing system for vaccine development in aquaculture

Abstract: Please cite this article as: Attaya A, Jiang Y, Secombes CJ, Wang T, Distinct response of immune gene expression in peripheral blood leucocytes modulated by bacterin vaccine candidates in rainbow trout Oncorhynchus mykiss: A potential in vitro screening and batch testing system for vaccine development in aquaculture, Fish and Shellfish Immunology (2019), doi:

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Cited by 10 publications
(12 citation statements)
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“…Freshly prepared PBL were seeded into 12-well cell culture plates (Greiner bio-one, UK) at 2 ml/well, and stimulated with phytohemagglutinin from red kidney bean Phaseolus vulgaris (PHA, 5 μg/ml, Sigma-Aldrich), rIL21 (200 ng/ml), and a formalin killed bacterin of Aeromonas salmonicida subsp. salmonicida MT423 cultured under iron depleted conditions (100 μg/ml, Attaya et al, 2019) for 24 h. These stimulation conditions are known to increase trout IL-22 transcript expression (Monte et al, 2011; Wang et al, 2011; Attaya et al, 2019). BD GolgiPlug™ containing brefeldin A (BD Biosciences, UK) was added 6 h before cell harvest to allow the accumulation of intracellular cytokines.…”
Section: Methodsmentioning
confidence: 99%
“…Freshly prepared PBL were seeded into 12-well cell culture plates (Greiner bio-one, UK) at 2 ml/well, and stimulated with phytohemagglutinin from red kidney bean Phaseolus vulgaris (PHA, 5 μg/ml, Sigma-Aldrich), rIL21 (200 ng/ml), and a formalin killed bacterin of Aeromonas salmonicida subsp. salmonicida MT423 cultured under iron depleted conditions (100 μg/ml, Attaya et al, 2019) for 24 h. These stimulation conditions are known to increase trout IL-22 transcript expression (Monte et al, 2011; Wang et al, 2011; Attaya et al, 2019). BD GolgiPlug™ containing brefeldin A (BD Biosciences, UK) was added 6 h before cell harvest to allow the accumulation of intracellular cytokines.…”
Section: Methodsmentioning
confidence: 99%
“…It is true that lethal sampling provides information on tissue-specific histopathological and immune responses. However, blood biomarkers for tissue-specific pathology [77] and immune response [78,79] in fish are continually improving. In combination with the sampling approach proposed here, we believe that significant improvements can be made in the understanding of disease dynamics at the population level, and the bases for differences in disease outcome, vaccine efficacy (protection correlates), and other biological phenotypes.…”
Section: Discussionmentioning
confidence: 99%
“…Indeed, studies on gut mucosal immunity in response to infection, vaccination, dietary additives and probiotics in fish have increased recently (31)(32)(33)(34)(35), where typically whole or segments of the gut were used for transcript analysis. Primary cell culture is a crucial technique in cell and molecular immunology, that provides excellent in vitro platform models for studying the cell immune repertoires and potentially the assessment of vaccine candidates [36]. The use of gut leucocytes for this purpose would seem a sensible first step for the in vitro testing of oral vaccine candidates.…”
Section: Introductionmentioning
confidence: 99%
“…However, the cell yield was still low compared to that obtained from other tissues, e.g. head kidney, spleen, gills and blood [36][37][38][39][40]. This low yield also prevents study of the cells from different intestine segments separately to explore whether immunological differences exist.…”
Section: Introductionmentioning
confidence: 99%
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