Distinct role of <i>Arabidopsis</i> mitochondrial P-type pentatricopeptide repeat protein-modulating editing protein, PPME, in <i>nad1</i> RNA editing

Leu, Kuan-Chieh; Hsieh, Ming‐Hsiun; Wang, Huei‐Jing; Hsieh, Hsun-Ping; Jauh, Guang‐Yuh

doi:10.1080/15476286.2016.1184384

Cited by 29 publications

(21 citation statements)

References 64 publications

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“…As PPR proteins of the pure subfamily such as NUWA have rarely been involved in editing (46) and to confirm that DYW2 and NUWA function primarily in RNA editing, total RNA-seq data were used to quantify organelle transcript splicing, processing, and accumulation in nuwa-2 and dyw2-1. The organelle transcriptome of dyw2-1 was highly impacted with 182 differentially expressed genes out of 239 and 21 differentially spliced introns out of 37 (Dataset S2 E-G).…”

Section: Resultsmentioning

confidence: 99%

Two interacting PPR proteins are major Arabidopsis editing factors in plastid and mitochondria

Guillaumot

López-Obando

Baudry

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

113

114

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RNA editing is converting hundreds of cytosines into uridines during organelle gene expression of land plants. The pentatricopeptide repeat (PPR) proteins are at the core of this posttranscriptional RNA modification. Even if a PPR protein defines the editing site, a DYW domain of the same or another PPR protein is believed to catalyze the deamination. To give insight into the organelle RNA editosome, we performed tandem affinity purification of the plastidial CHLOROPLAST BIOGENESIS 19 (CLB19) PPR editing factor. Two PPR proteins, dually targeted to mitochondria and chloroplasts, were identified as potential partners of CLB19. These two proteins, a P-type PPR and a member of a small PPR-DYW subfamily, were shown to interact in yeast. Insertional mutations resulted in embryo lethality that could be rescued by embryo-specific complementation. A transcriptome analysis of these complemented plants showed major editing defects in both organelles with a very high PPR type specificity, indicating that the two proteins are core members of E+-type PPR editosomes.

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Section: Resultsmentioning

confidence: 99%

Two interacting PPR proteins are major Arabidopsis editing factors in plastid and mitochondria

Guillaumot

López-Obando

Baudry

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

113

114

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“…Few of the examples include PPR (pentatricopeptide repeat containing gene; targeted through bra-miR161a-3p) which is a RNA binding protein and facilitate RNA editing or splicing of organelle genes5859. AP2 like transcription factor (targeted through bra-miR172c-3p) codes for a DNA binding protein and regulate G1-S transitions in shoot apical meristem60.…”

Section: Resultsmentioning

confidence: 99%

Thiourea priming enhances salt tolerance through co-ordinated regulation of microRNAs and hormones in Brassica juncea

Srivastava

Sablok

Hackenberg

et al. 2017

Sci Rep

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Activation of stress tolerance mechanisms demands transcriptional reprogramming. Salt stress, a major threat to plant growth, enhances ROS production and affects transcription through modulation of miRNAs and hormones. The present study delineates salt stress ameliorating action of thiourea (TU, a ROS scavenger) in Brassica juncea and provides mechanistic link between redox, microRNA and hormones. The ameliorative potential of TU towards NaCl stress was related with its ability to decrease ROS accumulation in roots and increase Na+ accumulation in shoots. Small RNA sequencing revealed enrichment of down-regulated miRNAs in NaCl + TU treated roots, indicating transcriptional activation. Ranking analysis identified three key genes including BRX4, CBL10 and PHO1, showing inverse relationship with corresponding miRNA expression, which were responsible for TU mediated stress mitigation. Additionally, ABA level was consistently higher till 24 h in NaCl, while NaCl + TU treated roots showed only transient increase at 4 h suggesting an effective stress management. Jasmonate and auxin levels were also increased, which prioritized defence and facilitated root growth, respectively. Thus, the study highlights redox as one of the “core” components regulating miRNA and hormone levels, and also strengthens the use of TU as a redox priming agent for imparting crop resilience to salt stress.

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“…P‐class PPR proteins participate in RNA stabilization, cleavage, splicing and translation (Barkan and Small, ). However, P‐class PPR proteins, which have a role in RNA editing, have been described (Doniwa et al ., ; Leu et al ., ; Andrés‐Colás et al ., ).…”

Section: Introductionmentioning

confidence: 99%

PRECOCIOUS1 (POCO1), a mitochondrial pentatricopeptide repeat protein affects flowering time in Arabidopsis thaliana

Emami

Kempken

2019

The Plant Journal

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Flowering is a vital developmental shift in plants from vegetative to reproductive phase. The timing of this shift is regulated by various linked genetic pathways including environmental cues and internal regulation. Here we report a role for an Arabidopsis gene, AT1G15480, which encodes a P-class pentatricopeptide repeat (PPR) protein, affecting flowering time. We show that AT1G15480 is localized to mitochondria. An AT1G15480 T-DNA insertion line exhibits an early-flowering phenotype, which is quite a rare phenotype among PPR mutants. The early-flowering phenotype was observed under both long and short days compared with wild type plants. Genetic complementation confirmed the observed phenotype. We therefore named the PPR protein PRECOCIOUS1 (POCO1). poco1 plants showed lower respiration, ATP content and higher accumulation of superoxide. Importantly, the quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that the expression of FLOWERING LOCUS C (FLC), which is a key floral repressor, was strongly downregulated in the poco1. Likewise, the expression level of the FLC positive regulator ABSCISIC ACID-INSENSITIVE 5 (ABI5) was reduced in the poco1. Consistent with the qRT-PCR results, poco1 plants showed reduced sensitivity to abscisic acid compared with wild type with respect to primary root growth and days to flowering. Furthermore, the poco1 mutation enhances the sensitivity to drought stress. Further analysis showed that POCO1 affects mitochondrial RNA editing. Taken together, our data demonstrate a remarkable function of POCO1 in flowering time and the abscisic acid signalling pathway.

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Distinct role of Arabidopsis mitochondrial P-type pentatricopeptide repeat protein-modulating editing protein, PPME, in nad1 RNA editing

Cited by 29 publications

References 64 publications

Two interacting PPR proteins are major Arabidopsis editing factors in plastid and mitochondria

Two interacting PPR proteins are major Arabidopsis editing factors in plastid and mitochondria

Thiourea priming enhances salt tolerance through co-ordinated regulation of microRNAs and hormones in Brassica juncea

PRECOCIOUS1 (POCO1), a mitochondrial pentatricopeptide repeat protein affects flowering time in Arabidopsis thaliana

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