Distinct Roles and Synergistic Function of FruM Isoforms in Drosophila Olfactory Receptor Neurons

Zhang, Ye; Ng, Renny; Neville, Megan C; Goodwin, Stephen F.; Su, Chih‐Ying

doi:10.1016/j.celrep.2020.108516

Cited by 11 publications

(34 citation statements)

References 54 publications

(95 reference statements)

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“…Strikingly, the genes with the altered expression tend to be localized on the cell membrane (Figure 3, GO: cellular component) and have functions in ion transport across membrane (Figure 3, GO: molecular function), and appear to be involved in the process of detecting and responding to olfactory stimuli (Figure 3, GO: biological process). This supports previous studies in providing a general mechanism for social experience, pheromone receptor signaling, and Fru M -dependent regulation of pheromone responsiveness of Or47b ORNs (Sethi et al, 2019; Zhang et al, 2020; Zhao et al, 2020). Furthermore, genes with oxidoreductase activity also have overlapping alterations across Or47b , Or67d, and fru M mutants, and many of these appear to contribute to insect hormone metabolism (Figure 3, GO: molecular function).…”

Section: Introductionsupporting

confidence: 90%

“…One functionally relevant gene among the genes that show differential regulation in pheromone receptor and fru M mutants is the Fru M target gene ppk25 , which previously was shown to modulate ORN responses in Or47b and Or67d neurons (Ng et al, 2019; Zhang et al, 2020). ppk25 belongs to a family of sodium channels that serve a variety of functions, from neuromodulation to detection of sensory cues.…”

Section: Discussionmentioning

confidence: 99%

“…Recent reports pointed to the function of DEG/ENaC channels known as pickpocket family of sodium channels that act in Or47b and Or67d ORNs to regulate responses to their ligands (Zhang et al, 2020). Fru M binding motifs have been identified around many of these ppk family members, such as ppk , ppk5 , ppk6, ppk15, ppk19 , ppk23 , ppk25, and ppk30 (Dalton et al, 2013; Neville et al, 2014; Vernes, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Fru M binding motifs have been identified around many of these ppk family members, such as ppk , ppk5 , ppk6, ppk15, ppk19 , ppk23 , ppk25, and ppk30 (Dalton et al, 2013; Neville et al, 2014; Vernes, 2014). Both ppk23 and ppk25 have been identified as necessary for changes of physiology in Or47b ORNs through Fru MB and Fru MC activity, respectively, with Fru MB having an antagonistic effect on physiology in Or67d ORNs (Zhang et al, 2020). We therefore first examined the expression levels of ppk genes in the antennae.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Social experience and pheromone receptor activity reprogram behavioral switch and neuromodulatory gene expression in sensory neurons

Volkan

Deanhardt

Duan

et al. 2021

Preprint

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Social experience and pheromone signaling in ORNs affect pheromone responses and male courtship behaviors in Drosophila, however, the molecular mechanisms underlying this circuit-level neuromodulation remain less clear. Previous studies identified social experience and pheromone signaling-dependent modulation of chromatin around behavioral switch gene fruitless, which encodes a transcription factor necessary and sufficient for male behaviors. To identify the molecular mechanisms driving social experience-dependent neuromodulation, we performed RNA-seq from antennal samples of mutant fruit flies in pheromone receptors and fruitless, as well as grouped or isolated wild-type males. We found that loss of pheromone detection differentially alters the levels of fruitless exons suggesting changes in splicing patterns. In addition, many Fruitless target neuromodulatory genes, such as neurotransmitter receptors, ion channels, and ion transporters, are differentially regulated by social context and pheromone signaling. Our results suggest that modulation of circuit activity and behaviors in response to social experience and pheromone signaling arise due to changes in transcriptional programs for neuromodulators downstream of behavioral switch gene function.

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Section: Introductionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Social experience and pheromone receptor activity reprogram behavioral switch and neuromodulatory gene expression in sensory neurons

Volkan

Deanhardt

Duan

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…Changes in FruM expression have been shown to affect male neurophysiology in ways predicted to alter behavior. 42 …”

Section: Increased Blood Ethanol Level Also Promotes Aggressionmentioning

confidence: 99%

Alcohol-induced Aggression

Atkinson

2021

J Exp Neurosci

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Intraspecies aggression is commonly focused on securing reproductive resources such as food, territory, and mates, and it is often males who do the fighting. In humans, individual acts of overt physical aggression seem maladaptive and probably represent dysregulation of the pathways underlying aggression. Such acts are often associated with ethanol consumption. The Drosophila melanogaster model system, which has long been used to study how ethanol affects the nervous system and behavior, has also been used to study the molecular origins of aggression. In addition, ethanol-induced aggression has been demonstrated in flies. Recent publications show that ethanol stimulates Drosophila aggression in 2 ways: the odor of ethanol and the consumption of ethanol both make males more aggressive. These ethanol effects occur at concentrations that flies likely experience in the wild. A picture emerges of males arriving on their preferred reproductive site—fermenting plant matter—and being stimulated by ethanol to fight harder to secure the site for their own use. Fly fighting assays appear to be a suitable bioassay for studying how low doses of ethanol reshape neural signaling.

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