Distinct Roles of Dendritic Cells and B Cells in Va14Ja18 Natural T Cell Activation In Vivo

Bezbradica, Jelena S.; Stanic, Aleksandar K.; Matsuki, Naoto; Bour-Jordan, Hélène; Bluestone, Jeffrey A.; Thomas, James W.; Unutmaz, Derya; Kaer, Luc Van; Joyce, Sebastian

doi:10.4049/jimmunol.174.8.4696

Cited by 142 publications

(152 citation statements)

References 64 publications

Supporting

Mentioning

137

Contrasting

Unclassified

Order By: Relevance

“…Efficient iNKT cell activation is key to the ignition of subsequent anti-infectious and antitumor responses, and DCs play a critical role due to their unique ability to activate iNKT cells (14,16). However, the nature of this ability has not been resolved.…”

Section: Discussionmentioning

confidence: 99%

“…7c). Inhibitory effects of B cells on the activation of iNKT cells were proposed as an explanation of the ineffectiveness of B cells as APCs for iNKT cells (16). To determine whether B cells inhibit the activation of iNKT cells, B cells were loaded with a high concentration (100 ng/ml) of ␣GalCer (␣GalCer/B cells).…”

Section: V␣14 Inkt Cells Are Potentiated By B Cells Overexpressing Wimentioning

confidence: 99%

“…The involvement of costimulatory signals and numerous cytokines has been suggested by some studies, yet questioned by others (8 -12). In contrast, activation of and cytokine production by iNKT cells can be differentially modulated by different types of APCs that express similar levels of CD1d and costimulatory molecules (13)(14)(15)(16). For instance, splenic, but not thymic APCs induce a strong ␣GalCer response (15).…”

mentioning

confidence: 99%

“…Also, whereas ␣GalCer-loaded dendritic cells (DCs) trigger potent and sustained IFN-␥ production by iNKT cells (14), the same Ag presented by B cells and macrophages resulted in a weak IL-4, but no IFN-␥ production (16). It is possible that DCs present additional stimulatory signals, or that some APCs are inhibitory for iNKT responses (16).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Efficient Activation of Vα14 Invariant NKT Cells by Foreign Lipid Antigen Is Associated with Concurrent Dendritic Cell-Specific Self Recognition

Ueno

Cho

et al. 2007

The Journal of Immunology

View full text Add to dashboard Cite

A burst release of cytokines by Vα14 invariant NKT (iNKT) cells upon their TCR engagement critically regulates innate and adaptive immune responses. However, it remains unclear in vivo why iNKT cells respond efficiently to microbial or intracellular lipid Ags that are at low levels or that possess suboptimal antigenicity. We found that dendritic cells (DCs) potentiated iNKT cells to respond to a minimal amount of ligand α-galactosylceramide (αGalCer) through CD1d-dependent autoreactive responses that require endosomal processing and CD1d trafficking. The ability of potentiation of NKT cells was DC specific and did not depend on costimulatory signals and IL-12 production by DCs. However, DCs that failed to synthesize a major endogenous lipid Ag isoglobotrihexosylceramide were unable to potentiate NKT cells for efficient activation. Further analysis showed that differences in the level and pattern of endogenous lipid Ag presentation differentiate DCs and B cells for effective potentiation and subsequent activation of iNKT cells in the presence of an exogenous Ag. Thus, CD1d-dependent potentiation by DCs may be crucial for iNKT cell-mediated immunity against infectious agents.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: V␣14 Inkt Cells Are Potentiated By B Cells Overexpressing Wimentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Efficient Activation of Vα14 Invariant NKT Cells by Foreign Lipid Antigen Is Associated with Concurrent Dendritic Cell-Specific Self Recognition

Ueno

Cho

et al. 2007

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…B lymphocytes suppress DC-mediated iNKT and NK cell activation and cell-cell contact is essential for this effect [35].…”

Section: Tim-1 Is Expressed On Cd4 + T Cells and T Helper 2 Cells Timentioning

confidence: 99%

The Naïve B Cells are the Lymphocytes with the Highest Anionic Phospholipid Binding Ratios

Moumaris¹

2012

TOIJ

View full text Add to dashboard Cite

Phospholipids (PLs) are the target for the clearance of apoptotic cells, and for the innate defense against malignant cancers or microbial infections. Several types of receptors for PLs were described on T lymphocytes, but not yet on B lymphocytes. To detect PLs binding lymphocytes two assays were used: a-FITC labelled liposomes, b-Petri dish coated with different PLs. The phenotype of lymphocytes was determined by FACS, using anti-CD mAb and anti-Ig polyclonal antibodies. Human lymphocytes bind PLs, the binding ratio decreases in the order phosphatidylserine (PS), cardiolipin (CL), phosphatidylethanolamine, and phosphatidylcholine. In healthy blood donors (n=17), 29% of B lymphocytes, 17% of NK cells, 9% of TCD4 + , and 4% of TCD8 + bind PS. Among B cells, the binding ratio varies with the subset, 49% for naïve cells (sIgM + sIgD + ), 42% for sIgA + , and 15% for sIgG + B cells. PL binding was not affected by the CD5 phenotype of B lymphocytes, nor by the HIV status of the donors (n=16) or the presence in the sera of IgG anti-CL antibodies. Pretreatment of lymphocytes with proteases inhibited the binding of PL, suggesting that binding is due to surface proteins. For each type of lymphocytes the various kinds of surface proteins which bind lipids, pattern recognition receptors, surface immunoglobulins, the lipid antigen presenting molecules CD1, and the CD1-restricted lipid antigen reactive TCR of NKT cells, are discussed.

show abstract

Characterization and Functional Analysis of Mouse Invariant Natural T (iNKT) Cells

2006

Self Cite

View full text Add to dashboard Cite

Invariant natural T (iNKT) cells are innate lymphocytes that recognize CD1d-restricted lipid antigens and have immunoregulatory properties. Human and mouse CD1d-restricted glycolipid antigen(s) and the iNKT cell functions they elicit are highly conserved, whereby, making the mouse an excellent animal model for understanding iNKT cell biology in vivo. This unit describes basic methods for the characterization and quantification (see Basic Protocol 1) and functional analysis of murine iNKT cells in vivo or in vitro (see Basic Protocols 2, 3, and 4). This unit also contains protocols that describe enrichment of iNKT cells (see Support Protocol 1), generation of CD1d-tetramer (see Support Protocol 2), and lipid antigen loading on cell-bound (see Support Protocol 3) or soluble (see Support Protocol 3) CD1d.

show abstract

Distinct Roles of Dendritic Cells and B Cells in Va14Ja18 Natural T Cell Activation In Vivo

Cited by 142 publications

References 64 publications

Efficient Activation of Vα14 Invariant NKT Cells by Foreign Lipid Antigen Is Associated with Concurrent Dendritic Cell-Specific Self Recognition

Efficient Activation of Vα14 Invariant NKT Cells by Foreign Lipid Antigen Is Associated with Concurrent Dendritic Cell-Specific Self Recognition

The Naïve B Cells are the Lymphocytes with the Highest Anionic Phospholipid Binding Ratios

Characterization and Functional Analysis of Mouse Invariant Natural T (iNKT) Cells

Contact Info

Product

Resources

About