Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR

Abstract

SUMMARYA total of 241 group A rotavirus-positive stool samples collected from diarrhoeic patients in Thailand between July 1988 and June 1991 were characterized for their serotypes by enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies and by a polymerase chain reaction (PCR). In July 1988-June 1989, serotype 1 was the most prevalent (63-4%), followed by serotype 4 (1IO0%) and serotype 2 (8-5 %). In July 1989-June 1990, 59-8 % were serotype 1, 24-3 % were serotype 2, and 6 1 % were serotype 3. In contrast, in July 1990-June 1991, serotype 3 was detected in the highest frequency (40-5 %), 29-9 % were serotype 1, and 27-3 % were serotype 2. Thus, a distinct yearly change of serotype distribution of rotavirus in Thailand was observed in the three consecutive years. In particular, it was of note that the prevalence of serotype 3 greatly increased, in contrast to the previous studies in which almost no serotype 3 rotaviruses were detected in the years 1983-8 in Thailand.Rotavirus is a major cause of diarrhoea in infants and young children worldwide [1,2]. The mortality rate in rotavirus diarrhoea is high in areas where therapy for dehydration is not sufficiently available. Because of the importance of this disease as a major global health problem, the World Health Organization considers the development of an effective rotavirus vaccine to have a high public health priority. However, the antigenic complexity of rotavirus has hampered the development of an effective vaccine [1,3]. Epidemiological studies on distribution of human rotavirus serotypes provide the basis for interpreting the results of field trials of candidate vaccines.

A long‐term survey on the distribution of the human rotavirus G type in Thailand

“…Regarding the G type distribution in Thailand, a yearly change in the distribution of the G type was also found in previous studies [Pongsuwanna et al, 1989[Pongsuwanna et al, , 1993. In 1988-1989, 1989-1992, G1 was most prevalent.…”

“…In 1988-1989, 1989-1992, G1 was most prevalent. In 1990and 1992-1993 and G2 were predominant, respectively. Thus, following the previous studies, a 19-year survey of the distribution of the G type in Thailand was carried out.…”

“…A total of 7,452 stool samples were collected from the same or different study sites in each period between July 1993and June 2007(1993 in Thailand (Table I).…”

Diarrhoea caused by rotavirus in a regional Peruvian hospital: determination of circulating genotypes

“…However, in multiple studies both techniques have shown variable sensitivity and specificity with a risk of misdiagnosis 13,14 . Polymerase Chain Reaction (PCR) has become the preferred method for human rotaviruses detection as well as genotype characterization in epidemiologic studies 15,16 .…”

Detecção e caracterização moleculares de <i>Picobirnavirus</i> bovino na região centro-sul do Brasil

“…A partir desse relato, diversos estudos descreveram a presença desses vírus em diferentes espécies de mamíferos: ratos (PEREIRA et al, 1988b;FREGOLENTE et al, 2009), bovinos (VANOPDENBOSCH & WELLEMANS, 1989GHOSH et al, 2009;BUZINARO et al, 2003;MALIK et al, 2011), búfalos (MONDAL et al, 2013MALIK et al, 2011), suínos (GATTI et al, 1989LUDERT et al, 1991;PONGSUWANNA et al, 1996;BÁNYAI et al, 2008), cavalos (BROWNING et al, 1991), coelhos (GALLIMORE et al, 1993;LUDERT et al, 1995), tamanduá bandeira (HAGA et al,1999), jumento, orangotango e tatu (MASACHESSI et al, 2007), macacos (WANG et al, 2012), leão (WOO et al, 2012;GILLMAN et al, 2013), puma, jaguar e gato (GILLMAN et al, 2013). Em aves, PBV já foi detectado em galinhas (LEITE et al, 1990;SILVIA et al, 2014), frangos de corte (TAMEHIRO et al, 2003), gansos (MASACHESSI et al, 2007), ema, pelicano e faisão (MASACHESSI et al, 2007); em répteis, PBV já foi encontrado em diferentes espécies de cobras (MASACHESSI et al, 2007 (PEREIRA et al, 1988a); na Itália, de 0,42% (CASCIO et al, 1996) e na Argentina, de 0,09% (GIORDANO et al, 2008).…”