Distinctive electrophoretic isoenzyme profiles in Saccharomyces sensu stricto

Abstract

Genetic variation among 35 strains representing the four currently recognized species of Saccharomyces sensu stricto (Saccharomyces cerevisiae, Saccharomyces bayanus, Saccharomyces pastorianuslcarlsbergensis and Saccharomyces paradoxus) was estimated by analysing the electrophoretic mobilities of nonspecific esterases, acid phosphatase, lactate dehydrogenase and g I ucose-6-p hosphate de h ydrogenase isoenzymes. Twenty-two electrophoretic types were identified, a result in agreement with the phenotypic and genetic polymorphisms reported for this group of yeasts. However, the four species were clearly distinguishable based on the patterns obtained using three of the enzymes assayed, the resolving power not being improved by the introduction of data correspondent t o lactate dehydrogenase. The overall diversity was higher among S. cerevisiae isolates, in contrast with 5. paradoxus which showed only two patterns, one of which was common to four of the five strains studied. Concordant results from the application of the method and DNA hybridization experiments demonstrate its value for identification purposes.

Genetic Polymorphism in Wine Yeasts: Mechanisms and Methods for Its Detection

“…Simplified biochemical methods have also been developed based on fermentation and assimilation characteristics (Rohm, 1990). Other methods have been based on the analysis of total proteins in the cell (Van Vuuren and Meer, 1987; Vancanneyt et al, 1991), isoenzymic patterns (Duarte et al, 1999), fatty acid analysis using gas chromatography (Cottrell et al, 1986; Tredoux et al, 1987; Moreira da Silva et al, 1994) or, more recently, the application of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) for yeast differentiation (Blättel et al, 2013; Agustini et al, 2014), especially in the domain of medical sciences for the identification of pathogenic microorganisms (Stevenson et al, 2010; van Veen et al, 2010). However, DNA-based methods are currently the most widely used techniques for yeast differentiation.…”

Role of social wasps in Saccharomyces cerevisiae ecology and evolution

“…Polsinelli et al reported that, before maturation, grapes are almost free of S. cerevisiae (∼0.05%), whereas 25% of ripe damaged grapes harbor such cells (6,7). Following this pioneering study, S. cerevisiae strains have been isolated from several natural sources (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22). However, these reports are restricted to warm seasons, when ripe fruit is available, or to post harvest.…”

Protoplast Fusion between Geotrichum candidium and Phanerochaete chrysosporium to Produce Fusants for Corn Stover Fermentation

“…Electrophoretic isoenzyme analysis has been widely applied as a standard method in microbial taxonomy (Duarte et al 1999). The isoenzyme pattern can display the taxonomical characteristics of a microbe and disclose the genetical distance within related microbes.…”