Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells

Scaletsky, Isabel C. A.; Silva, M L; Trabulsi, Luíz Rachid

doi:10.1128/iai.45.2.534-536.1984

Cited by 524 publications

(301 citation statements)

References 11 publications

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“…Phenotypic and genotypic assays have been developed for detection of E. coli harboring Afa/Dr adhesins. On the basis that pathogenic E. coli strains attach to HeLa cells in different patterns (localized, diffuse, or aggregative), an adhesion assay has been proposed for the detection of the mannose-resistant diffuse adhesiveness of DAEC strains onto cultured epithelial Hep-2 or HeLa cells (307,308,365). The adhesion assay is not specific for Afa/Dr DAEC detection, since other pathogenic E. coli strains, including DA EPEC strains (26-29), have been reported to develop the diffuse phenotype of adhesion without the presence of Afa/Dr adhesins.…”

Section: Diagnosismentioning

confidence: 99%

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Pathogenesis of Afa/Dr Diffusely AdheringEscherichia coli

2005

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Over the last few years, dramatic increases in our knowledge about diffusely adhering Escherichia coli (DAEC) pathogenesis have taken place. The typical class of DAEC includes E. coli strains harboring AfaE-I, AfaE-II, AfaE-III, AfaE-V, Dr, Dr-II, F1845, and NFA-I adhesins (Afa/Dr DAEC); these strains (i) have an identical genetic organization and (ii) allow binding to human decay-accelerating factor (DAF) (Afa/DrDAF subclass) or carcinoembryonic antigen (CEA) (Afa/DrCEA subclass). The atypical class of DAEC includes two subclasses of strains; the atypical subclass 1 includes E. coli strains that express AfaE-VII, AfaE-VIII, AAF-I, AAF-II, and AAF-III adhesins, which (i) have an identical genetic organization and (ii) do not bind to human DAF, and the atypical subclass 2 includes E. coli strains that harbor Afa/Dr adhesins or others adhesins promoting diffuse adhesion, together with pathogenicity islands such as the LEE pathogenicity island (DA-EPEC). In this review, the focus is on Afa/Dr DAEC strains that have been found to be associated with urinary tract infections and with enteric infection. The review aims to provide a broad overview and update of the virulence aspects of these intriguing pathogens. Epidemiological studies, diagnostic techniques, characteristic molecular features of Afa/Dr operons, and the respective role of Afa/Dr adhesins and invasins in pathogenesis are described. Following the recognition of membrane-bound receptors, including type IV collagen, DAF, CEACAM1, CEA, and CEACAM6, by Afa/Dr adhesins, activation of signal transduction pathways leads to structural and functional injuries at brush border and junctional domains and to proinflammatory responses in polarized intestinal cells. In addition, uropathogenic Afa/Dr DAEC strains, following recognition of β1 integrin as a receptor, enter epithelial cells by a zipper-like, raft- and microtubule-dependent mechanism. Finally, the presence of other, unknown virulence factors and the way that an Afa/Dr DAEC strain emerges from the human intestinal microbiota as a “silent pathogen” are discussed

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Section: Diagnosismentioning

confidence: 99%

“…DAEC strains have been identified from their diffuse adherence (DA) pattern on cultured epithelial HEp-2 as well as HeLa cells (307,308,365), and they appear to form a heterogeneous group (91,308). The first class of DAEC strains includes E. coli strains that harbor Afa/Dr adhesins (Afa/Dr DAEC) (322).…”

Section: Introductionmentioning

confidence: 99%

Pathogenesis of Afa/Dr Diffusely AdheringEscherichia coli

2005

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“…Three patterns of adherence to tissue culture cells among faecal isolates of Escherichia coli have been identi-¢ed : localised adherence (LA), di¡use adherence (DA), and aggregative adherence (AA) [1,2]. Gomes et al [3] have recently described a distinctive pattern of adherence characterised by bacteria attaching on both coverslip and HeLa cell surfaces forming long chain aggregates.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of HEp-2 cell-adherent Escherichia coli and characterisation of enteroaggregative E. coli and chain-like adherent E. coli isolated from children with and without diarrhoea, in Londrina, Brazil

Gioppo

2000

FEMS Microbiology Letters

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A total of 919 Escherichia coli isolates from 125 children with diarrhoea (cases) and 98 controls were assayed for adherence to HEp-2 cells. Localised adherence was found only in isolates from cases. Diffuse, aggregative (AA), chain-like adherence (CLA) and variants of the AA pattern were found in both cases and controls. The AA isolates were tested for gene sequences associated with enteroaggregative E. coli (EAEC). Only 25% of the isolates hybridised with the EAEC probe, and the aafA, astA and pet gene sequences were found in 7.9%, 44.7% and 7.9% of the isolates, respectively. The aggA gene was not found, although 7.9% were positive for aggC. The CLA isolates reacted with the EAEC probe (55.6%), and the aggC, astA and pet gene sequences were found in 66.7%, 33.3% and 11.1%, respectively. The aggR (55.6%), aspU (55.6%), shf (33.3%) and she (22.2%) genes were also found in CLA isolates. ß

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“…Enteropathogenic Escherichia coli (EPEC) is one of the main causes of infant diarrhea in children in developing countries [1,2]. EPEC adheres to epithelial cells forming microcolonies, a pattern called lo-calized adherence (LA) [3]. In addition, it produces a distinct histopathological lesion on the intestinal mucosa referred to as the attaching and e¡acing (AE) lesion [4], which is characterized by e¡acement of microvilli and intimate adherence of the bacterium to the apical surface of the epithelial cells [4].…”

Section: Introductionmentioning

confidence: 99%

Lack of expression of bundle-forming pili in some clinical isolates of enteropathogenic Escherichia coli (EPEC) is due to a conserved large deletion in the bfp operon

Bortolini

1999

FEMS Microbiology Letters

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Enteropathogenic Escherichia coli (EPEC) produces a plasmid-encoded type IV pilus, called the bundle-forming pilus (BFP), involved in the formation of the localized adhesion onto epithelial cells. In this study, we demonstrate that clinical isolates of serotypes O128ab:H2 and O119:H2 contain a ca. 13-kb deletion in the bfp operon, resulting in a lack of expression of these pili. An IS sequence with homology to the IS66 of Agrobacterium tumefaciens replaced the deleted bfp genes. These results suggest that the bfp operon was deleted through a transpositional event and that other adherence factors may mediate attachment of these bacteria to the host cells. ß

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Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells

Cited by 524 publications

References 11 publications

Pathogenesis of Afa/Dr Diffusely AdheringEscherichia coli

Pathogenesis of Afa/Dr Diffusely AdheringEscherichia coli

Prevalence of HEp-2 cell-adherent Escherichia coli and characterisation of enteroaggregative E. coli and chain-like adherent E. coli isolated from children with and without diarrhoea, in Londrina, Brazil

Lack of expression of bundle-forming pili in some clinical isolates of enteropathogenic Escherichia coli (EPEC) is due to a conserved large deletion in the bfp operon

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