Distinctive responses of heart muscle and non-muscle cells to oxygen and glucose deprivation as regards phospholipid fatty acids

Freyss-Beguin, M; Brussel, Elisabeth Millanvoye-Van; Lechat, Geneviève Griffaton Paul

doi:10.1016/0006-2952(85)90544-1

Cited by 5 publications

(7 citation statements)

References 16 publications

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“…viously in heart cell cultures derived from 3-day-old Sprague-Dawley rats (Van Brussel and Freyss-Beguin, 1979;Freyss-Beguin and Van Brussel, 1985). Similarly, fatty acid composition of the phospholipids was different in F and M cells within the same genotype, as previously reported in the Sprague-Dawley rat (FreyssBeguin and Van Brussel, 1985).…”

Section: Discussionsupporting

confidence: 57%

“…Heart-cell cultures were prepared simultaneously from both groups as previously reported (Freyss-Beguin et al, 1985). Briefly, cells were isolated from minced hearts by repeated trypsinization at 23°C with 0.75-0.5%0 trypsin solution (swine pancreas trypsin, twice crystallized, Choay-France), with each treatment lasting 5 minutes.…”

Section: Cell Culturesmentioning

confidence: 99%

“…In another series of experiments, extracted lipids were used for the analysis of the phospholipid fatty acids, as previously described (Freyss-Beguin et al, 1985). Briefly, extracted lipids were fractionated on a silicic acid column (silicic AR CC-4, Mallinkrodt Inc.) according to Dittmer and Wells (1969).…”

Section: Phospholipid Fatty Acid Analysismentioning

confidence: 99%

“…In the whole heart, there are two types of cells that are also found in culture: muscle cells (M), which beat and have high P-oxydative activities, and nonmuscle cells or fibroblasts (F), which do no beat and exhibit low p-oxidation levels (Van Brussel and Freyss-Beguin, 1979). It is possible to culture them separately by means of a differential attachment technique and by the addition of an antimitotic drug to M cell culture (Freyss-Beguin et al, 1985).…”

mentioning

confidence: 99%

“…In addition, we have examined the phospholipid composition of these cells, as it has been reported that in liver and adipose tissue in adult animals, the acyl chain composition of membrane phospholipids is altered in oblob mice York et al, 1982) and fatfa rats (Wahle, 1974). We previously reported that in the Sprague-Dawley rat, a difference between the phospholipid fatty acid composition of M and F cultures exists, with the higher proportion of polyunsaturated fatty acids in M cells than in F cells (Freyss-Beguin et al, 1985). The possibility that the genotype could modify the membrane phospholipid composition of M and F cells in culture has been investigated.…”

mentioning

confidence: 99%

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Abnormal fatty acid utilization by cultured cardiac cells from 7‐day‐old obese zucker rats

Quignard‐Boulangé¹,

Freyss-Beguin²,

Brigant³

et al. 1989

Journal Cellular Physiology

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Fatty acid utilization by muscle and nonmuscle heart cells in culture has been investigated in the 7-day-old Zucker rat to determine if this tissue could contribute to the lower energy expenditure reported in obese rats at the onset of obesity. The partitioning of oleate to oxidation and esterification products and the effect of genotype on this partitioning according to cell types were studied. Results showed that the fatty acid beta-oxidation and its esterification in neutral lipid was decreased by 30% in beating muscle cells from obese animals when compared with those from lean animals. In contrast, nonmuscle cells exhibited a decreased beta-oxidation alone. A similar fatty acid composition of the phospholipids was found in non-muscle cells of obese animals and their lean litter mates. In muscle cultures, palmitic and oleic acids are lower in cells of obese rats than in those of lean rats. The present study indicates that a defect in energy metabolism could be found in heart cells at the onset of obesity, suggesting that this defect is determined by intrinisic factor(s).

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Section: Discussionsupporting

confidence: 57%

Section: Cell Culturesmentioning

confidence: 99%

Section: Phospholipid Fatty Acid Analysismentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Abnormal fatty acid utilization by cultured cardiac cells from 7‐day‐old obese zucker rats

Quignard‐Boulangé¹,

Freyss-Beguin²,

Brigant³

et al. 1989

Journal Cellular Physiology

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Nonesterified fatty acid accumulation and release during heart muscle‐cell (myocyte) injury: Modulation by extracellular “acceptor”

Janero

Burghardt

1989

Journal Cellular Physiology

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Long-chain nonesterified fatty acid (NEFA) accumulation in the heart muscle cell (myocyte) and NEFA release to the extracellular milieu are considered contributors to the pathogenesis of myocardial injury in a number of cardiovascular disease states. Reported here is a study of the factors which influence and control the interactions among NEFA formation, intracellular NEFA accumulation, and NEFA release to the extracellular compartment by the irreversibly injured myocyte. Under conditions of metabolic inhibition, neonatal rat myocytes in primary monolayer culture became virtually depleted of ATP within 8 h. The metabolically inhibited myocytes evidenced membrane phospholipid degradation and a resultant net accumulation of NEFA produced thereby in the extracellular medium. However, under conditions of nutrient deprivation, the injured myocytes retained the NEFA produced from phospholipid catabolism intracellularly and did not release it to the culture medium, although the extent of myocyte ATP depletion was the same as it had been from metabolic inhibition. Serum could elicit, in a concentration-dependent fashion, the quantitative release of NEFA from metabolically inhibited myocytes to the culture medium but did not influence the net production of NEFA by the injured cells. Similarly, NEFA release from nutrient-deprived myocytes incubated in serum-free, substrate-free medium or in physiological buffer could be induced by supplementing the medium or buffer with bovine serum albumin (BSA), and the extent of NEFA release, but not NEFA formation, was dependent upon the extracellular BSA concentration. No manipulations to media other than changing their serum content or supplementing them with BSA were found to influence the disposition of NEFA produced during phospholipid catabolism in the irreversibly injured, ATP-depleted myocyte. Therefore, although progressive metabolic compromise in the myocyte was correlated with increasing, net NEFA formation, the distribution of the NEFA between the intracellular and the extracellular compartments was not determined by the magnitude of ATP loss or by the nature or duration of at least two injury stimuli, metabolic inhibition and nutrient deprivation. Rather, the net release of NEFA from the ATP-depleted myocyte to the culture medium and the consequent reduction of intracellular myocyte NEFA overload were critically and causally dependent upon the presence and concentration of extracellular NEFA "acceptor". The influence of acceptor on the mobilization of NEFA from the injured myocyte has implications regarding the use of NEFA release as an index of myocyte pathology and could serve to modify the progression and extent of myocardial injury in vivo.

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3,4-Diaminopyridine partially reverses phospholipid breakdown induced by oxygen deprivation of cultured myocardial cells

Freyss-Beguin

Brussel

Griffaton

et al. 1986

Biochemical Pharmacology

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Distinctive responses of heart muscle and non-muscle cells to oxygen and glucose deprivation as regards phospholipid fatty acids

Cited by 5 publications

References 16 publications

Abnormal fatty acid utilization by cultured cardiac cells from 7‐day‐old obese zucker rats

Abnormal fatty acid utilization by cultured cardiac cells from 7‐day‐old obese zucker rats

Nonesterified fatty acid accumulation and release during heart muscle‐cell (myocyte) injury: Modulation by extracellular “acceptor”

3,4-Diaminopyridine partially reverses phospholipid breakdown induced by oxygen deprivation of cultured myocardial cells

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