Distinctive Test for α-Amino-acids in Paper Chromatography

Crumpler, H. R.; Dent, C. E.

doi:10.1038/164441a0

Cited by 113 publications

(57 citation statements)

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“…It was identified as taurine after the following observations: The location of the spot coincided with that of known taurine. It withstood the copper treatment ( 5 ) and was, therefore, not an alpha-amino acid. Aliquots of the acid hydrolysates of some of the species which contained this substance were diluted and put through two successive columns of 1 :2 cupric carbonate-alumina (w/w) ( 3 6 ) .…”

Section: Resultsmentioning

confidence: 99%

NUTRITIVE VALUE OF FISH^a I. NICOTINIC ACID, RIBOFLAVIN, VITAMIN B₁₂, AND AMINO ACIDS OF VARIOUS SALT‐WATER SPECIES

Teeri

Loughlin

Josselyn

1957

Journal of Food Science

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The consumption of fish and other marine products has always been an important factor in the economy and in the nutrition of coastal populations. Recently there has been a trend toward increased fish consumption, particularly in school lunch programs (22, 25, 2 7 ) . Fish cookery demonstrations before school lunch personnel in Maine, New Hampshire, Vermont, Rhode Island, and Connecticut resulted in a 59% increase in fish consumption in the sehool lunch program. It is predicted that one result of this campaign Will be an increased use of fish in the area as a whole ( 2 5 ) , which, Considering the high nutritive value of fishery products, should benefit the nutritional status of the population.Available information on the vitamin and amino acid content of fish includes reports on the nutritive value of fish from Michigan waters (19, 22, 23), the levels of certain vitamins in Florida-produced foods (12), and the B-vitamin content of certain marine products ( I , 17). Studies of specific nutrients in certain fishery products have include ascorbic acid (12, 2 4 ) , thiamine (13, 23, 35), nicotinic acid (6, 22, 2 6 ) , riboflavin ( 2 , 25, I S ) , vitamin Blz (10, 26, 30, 34, 3 8 ) , and various amino acids (7, 9, 28, 29, 33). Similar information with respect to the nicotinic acid, riboflavin, vitamin BIZ, and amino acids of various species commonly consumed in New England and other areas is reported herein. Included are certain marine products, such as lobsters, which are of particular importance to the New England area. EXPERIMENTAL PROCEDUREMarket-fresh samples of various species of fish, including shellfish, which are commonly consumed in New England, were analyzed qualitatively for amino acids and quantitatively for nicotinic acid, riboflavin, vitamin BI?, total nitrogen, and total solids. A small number of canned samples was also analyzed.The edible portion of the fish muscle, blended in a Waring blender, was used for analysis. With the small fish, several were blended together. The vitamin analyses were carried out on hot-water extracts (autoclaved) of the blended mixtures by standard quantitative microbiological procedures ( 8 ) , using Lactobacillus arabinosus 17-5 (A. T. C. C. No. 8014) for nicotinic acid, Lactobacillus casei (No. 7469) for riboflavin, and Lactobacillus Zsichmannii (No. 7830) for vitamin Bu. Protein was determined by the macro-Kjeldahl nitrogen procedure on 2-g. aliquots of the blended fish, and moisture determinations were made by heating weighed samples in an oven at approximately 103" C. for 48 hours.

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Section: Resultsmentioning

confidence: 99%

NUTRITIVE VALUE OF FISH^a I. NICOTINIC ACID, RIBOFLAVIN, VITAMIN B₁₂, AND AMINO ACIDS OF VARIOUS SALT‐WATER SPECIES

Teeri

Loughlin

Josselyn

1957

Journal of Food Science

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“…It could be distinguished from methionine sulphoxide however by the fact that it could not be further oxidized to the sulphone, and from the sarcosine by its failure to form a copper complex under the conditions described by Crumpler & Dent (1949). It had, in fact, several distinctive properties which indicated that it did not correspond to any of the already known amino-acids.…”

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confidence: 80%

FAMILY STUDIES ON THE URINARY EXCRETION OF SS-Aminoisobutyric ACID

Harris¹

1952

Annals of Eugenics

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“…Non-a-amino-acids were detected by the copper carbonate, technique of Crumpler and Dent, (1949), using butanol/pyridine/ water (1:1:1) and phenol/water (4:1) as developing solvents. Figure 1 shows a map of the amino acids and other ninhydrin-reacting substances and Figure 2 a map of the non-a-amino acids.…”

Section: Identification Of Ninhydrin-reacting Substancesmentioning

confidence: 99%

Application of Paper Chromatography to Avian Pathology

Chubb

1959

Poultry Science

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Distinctive Test for α-Amino-acids in Paper Chromatography

Cited by 113 publications

References 4 publications

NUTRITIVE VALUE OF FISH^a I. NICOTINIC ACID, RIBOFLAVIN, VITAMIN B₁₂, AND AMINO ACIDS OF VARIOUS SALT‐WATER SPECIES

NUTRITIVE VALUE OF FISH^a I. NICOTINIC ACID, RIBOFLAVIN, VITAMIN B₁₂, AND AMINO ACIDS OF VARIOUS SALT‐WATER SPECIES

FAMILY STUDIES ON THE URINARY EXCRETION OF SS-Aminoisobutyric ACID

Application of Paper Chromatography to Avian Pathology

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Distinctive Test for α-Amino-acids in Paper Chromatography

Cited by 113 publications

References 4 publications

NUTRITIVE VALUE OF FISHa I. NICOTINIC ACID, RIBOFLAVIN, VITAMIN B12, AND AMINO ACIDS OF VARIOUS SALT‐WATER SPECIES

NUTRITIVE VALUE OF FISHa I. NICOTINIC ACID, RIBOFLAVIN, VITAMIN B12, AND AMINO ACIDS OF VARIOUS SALT‐WATER SPECIES

FAMILY STUDIES ON THE URINARY EXCRETION OF SS-Aminoisobutyric ACID

Application of Paper Chromatography to Avian Pathology

Contact Info

Product

Resources

About

NUTRITIVE VALUE OF FISH^a I. NICOTINIC ACID, RIBOFLAVIN, VITAMIN B₁₂, AND AMINO ACIDS OF VARIOUS SALT‐WATER SPECIES

NUTRITIVE VALUE OF FISH^a I. NICOTINIC ACID, RIBOFLAVIN, VITAMIN B₁₂, AND AMINO ACIDS OF VARIOUS SALT‐WATER SPECIES