Distinguished Photons: A Review of In Vivo Spectral Fluorescence Imaging in Small Animals

Mansfield, James R.

doi:10.2174/138920110792246474

Cited by 18 publications

(9 citation statements)

References 49 publications

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“…The concept of spectral unmixing is based on the "signature" emission intensity that each fluorophore has at certain wavelengths, providing the fluorophore with its own specific emission spectrum (28). Using spectral unmixing, the signal is decomposed into a collection of predefined spectra that is used to determine the individual contribution of each fluorophore.…”

Section: Strategies To Distinguish the Target-specific Fluorescence Smentioning

confidence: 99%

Optical Image-Guided Cancer Surgery: Challenges and Limitations

Keereweer

Driel

Snoeks

et al. 2013

Clinical Cancer Research

229

209

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Optical image-guided cancer surgery is a promising technique to adequately determine tumor margins by tumor-specific targeting, potentially resulting in complete resection of tumor tissue with improved survival. However, identification of the photons coming from the fluorescent contrast agent is complicated by autofluorescence, optical tissue properties, and accurate fluorescent targeting agents and imaging systems. All these factors have an important influence on the image that is presented to the surgeon. Considering the clinical consequences at stake, it is a prerequisite to answer the questions that are essential for the surgeon. What is optical image-guided surgery and how can it improve patient care? What should the oncologic surgeon know about the fundamental principles of optical imaging to understand which conclusions can be drawn from the images? And how do the limitations influence clinical decision making? This article discusses these questions and provides a clear overview of the basic principles and practical applications. Although there are limitations to the intrinsic capacity of the technique, when practical and technical surgical possibilities are considered, optical imaging can be a very powerful intraoperative tool in guiding the future oncologic surgeon toward radical resection and optimal clinical results.

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Section: Strategies To Distinguish the Target-specific Fluorescence Smentioning

confidence: 99%

Optical Image-Guided Cancer Surgery: Challenges and Limitations

Keereweer

Driel

Snoeks

et al. 2013

Clinical Cancer Research

229

209

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“…Spectral multiplexing of fluorophores in optical techniques requires the collection of dense spectral data sets for efficient unmixing of independent signals [4]. Such data sets are acquired sequentially one wavelength at a time, leading to relatively long acquisition times, especially for whole body applications.…”

Section: Introductionmentioning

confidence: 99%

Monte Carlo based method for fluorescence tomographic imaging with lifetime multiplexing using time gates

Chen

Venugopal

Intes

2011

Biomed. Opt. Express

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Time-resolved fluorescence optical tomography allows 3-dimensional localization of multiple fluorophores based on lifetime contrast while providing a unique data set for improved resolution. However, to employ the full fluorescence time measurements, a light propagation model that accurately simulates weakly diffused and multiple scattered photons is required. In this article, we derive a computationally efficient Monte Carlo based method to compute time-gated fluorescence Jacobians for the simultaneous imaging of two fluorophores with lifetime contrast. The Monte Carlo based formulation is validated on a synthetic murine model simulating the uptake in the kidneys of two distinct fluorophores with lifetime contrast. Experimentally, the method is validated using capillaries filled with 2.5nmol of ICG and IRDye™800CW respectively embedded in a diffuse media mimicking the average optical properties of mice. Combining multiple time gates in one inverse problem allows the simultaneous reconstruction of multiple fluorophores with increased resolution and minimal crosstalk using the proposed formulation.

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“…FMT typically offers higher sensitivity compared to other modalities, such as micro CT or micro MRI [3] and is relatively inexpensive. FMT is also capable of imaging various molecular targets using multiple agents with different spectral or lifetime characteristics [4–6]. …”

Section: Introductionmentioning

confidence: 99%

Ex VivoFluorescence Molecular Tomography of the Spine

Pimpalkhare

Chen

Venugopal

et al. 2012

International Journal of Biomedical Imaging

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We investigated the potential of fluorescence molecular tomography to image ex vivo samples collected from a large animal model, in this case, a dog spine. Wide-field time-gated fluorescence tomography was employed to assess the impact of multiview acquisition, data type, and intrinsic optical properties on the localization and quantification accuracy in imaging a fluorescent inclusion in the intervertebral disk. As expected, the TG data sets, when combining early and late gates, provide significantly better performances than the CW data sets in terms of localization and quantification. Moreover, the use of multiview imaging protocols led to more accurate localization. Additionally, the incorporation of the heterogeneous nature of the tissue in the model to compute the Jacobians led to improved imaging performances. This preliminary imaging study provides a proof of concept of the feasibility of quantitatively imaging complex ex vivo samples nondestructively and with short acquisition times. This work is the first step towards employing optical molecular imaging of the spine to detect and characterize disc degeneration based on targeted fluorescent probes.

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Distinguished Photons: A Review of In Vivo Spectral Fluorescence Imaging in Small Animals

Cited by 18 publications

References 49 publications

Optical Image-Guided Cancer Surgery: Challenges and Limitations

Optical Image-Guided Cancer Surgery: Challenges and Limitations

Monte Carlo based method for fluorescence tomographic imaging with lifetime multiplexing using time gates

Ex VivoFluorescence Molecular Tomography of the Spine

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