Distinguishing African and European honeybee matrilines using amplified mitochondrial DNA.

Hall, H. Glenn; Smith, Deborah R.

doi:10.1073/pnas.88.10.4548

Cited by 166 publications

(148 citation statements)

References 21 publications

Supporting

Mentioning

131

Contrasting

Unclassified

Order By: Relevance

“…It can be inferred from worker data that there was no colony or swarm with a homozygous queen for these European alleles. Heterozygous The banding pattern of the PCR amplification of the LRS gene and restriction enzyme digestion with EcoP.J was similar to the ones reported by Hall & Smith (1991). The size of the amplified region was approximately 738 bp and samples containing an EcoRl restriction site produced two fragments of 483 bp and 251 bp, respectively.…”

Section: Morphometrysupporting

confidence: 58%

“…Distinction between the European or African origins of mitochondrial DNA was made by PCR amplification of polymorphic regions of mitochondrial DNA, following the methods of Hall & Smith (1991). For this, total DNA of one individual worker was isolated by the procedure of Hall 1990.…”

Section: Morphometrymentioning

confidence: 99%

“…For this, total DNA of one individual worker was isolated by the procedure of Hall 1990. PCR amplification was performed using two of the primer pairs recommended by Hall & Smith (1991).…”

Section: Morphometrymentioning

confidence: 99%

See 2 more Smart Citations

Morphometric isozymic and mitochondrial variability of Africanized honeybees in Costa Rica

Lobo

1995

Heredity

View full text Add to dashboard Cite

Wing morphometry, isozymic variation and mitochondrial RFLPs were studied in samples of feral Africanized bees collected at three different locations in Costa Rica, two of them in the lowlands (Guanacaste and Golfito regions) and the other in the Central Valley. These data revealed a predominantly African origin at all locations. However, significant heterogeneity was found between the two lowland sites and the Central Valley for some isozyme markers as well as for the RFLPs considered in this study. Both isozymic and mitochondrial DNA European markers were more frequent in the Central Valley. This could be the result of different opportunities for European gene introgression at the beginning of the migration of Africanized honeybees. It is shown that different gene frequencies of mitochondrial and nuclear European genetic markers may exist in Africanized bee populations, and that this may result from different selection pressures among colonies with African and European queens.

show abstract

Section: Morphometrysupporting

confidence: 58%

Section: Morphometrymentioning

confidence: 99%

See 1 more Smart Citation

Morphometric isozymic and mitochondrial variability of Africanized honeybees in Costa Rica

Lobo

1995

Heredity

View full text Add to dashboard Cite

show abstract

“…DNA sequencing, PCR-RFLP and RAPD analyses have been used for population genetic and systematic studies in several taxa (White et al, 1996;Hall and Smith, 1991;Klinbunga et al, 2000;Sittipraneed et al, 2001). Using these approaches, several species-specific markers were observed in T. clareae and T. koenigerum.…”

Section: Discussionmentioning

confidence: 99%

Genetic differences betweenTropilaelaps clareaeandTropilaelaps koenigerumin Thailand based on ITS and RAPD analyses

et al. 2003

View full text Add to dashboard Cite

-Tropilaelaps koenigerum was first reported in Thailand. Species-diagnostic markers of bee mites, T. clareae and T. koenigerum were examined by sequencing of ITS and RAPD analysis. A lack of intraspecific polymorphism within T. clareae and T. koenigerum ITS was observed. At an interspecific level, 19 substitutions were found. Sequence divergence between ITS of these bee mites was 3.82%. A 5 bp (TTCTC) insertion was found in T. koenigerum. Based on ITS sequences, identification of these mites was simplified to restriction analysis of the amplified ITS with Mse I and/or Sau3A I. RAPD analysis using primers OPA07, OPA11 and OPA12 revealed 16 and 20 species-specific markers of T. clareae and T. koenigerum, respectively. A UPGMA phenogram based on genetic distance between pairs of geographic samples indicated clear differentiation between T. clareae and T. koenigerum genetically. Partial differentiation of T. clareae from different host species was observed.Tropilaelaps / bee parasite / RAPD / ITS / species-diagnostic marker / genetic diversity

show abstract

“…Haplotype identification was conducted by PCR analyzing the 485-bp section of the cytochrome bgene (Crozier et al, 1991) and the 783-bp of the large ribosomal subunit (ls rRNA) gene (Hall and Smith, 1991). The fragments were amplified using a modified protocol of Saiki et al (1988) and the PCR conditions were those of Nielsen et al (1999Nielsen et al ( , 2000.…”

Section: Analysis Of Haplotypesmentioning

confidence: 99%

Frequency of European and African-derived morphotypes and haplotypes in colonies of honey bees (Apis mellifera) from NW Mexico

et al. 2008

View full text Add to dashboard Cite

-Africanized honey bees (AHBs Apis mellifera) have been reported in NW Mexico since the mid 90s, but no study on the process of admixture with local European honey bees has been conducted. Morphometrics and haplotype analyses were used to investigate the frequency of African markers in honey bees from Sonora (SON), the north and south of Baja California (BCN and BCS). Morphometrics identified 42% of the samples from SON, 44% from BCN and 15% of BCS as Africanized. Honey bees from BCS had larger body size and formed a separate cluster from BCN and SON which were similar to each other. The molecular analysis revealed a higher frequency of African-derived haplotypes in SON (48%) and BCN (50%) compared to BCS (21%). The morphometric and molecular evidence suggests that the colonization of BCS by AHBs may be recent. Nest and food availability in desert areas and beekeeping practices are evoked to explain the reduced introgression of African genes into honey bee populations from this region of Mexico.Apis mellifera / Africanized honey bee / Baja California / hybridization / haplotype / morphometrics

show abstract

Distinguishing African and European honeybee matrilines using amplified mitochondrial DNA.

Cited by 166 publications

References 21 publications

Morphometric isozymic and mitochondrial variability of Africanized honeybees in Costa Rica

Morphometric isozymic and mitochondrial variability of Africanized honeybees in Costa Rica

Genetic differences betweenTropilaelaps clareaeandTropilaelaps koenigerumin Thailand based on ITS and RAPD analyses

Frequency of European and African-derived morphotypes and haplotypes in colonies of honey bees (Apis mellifera) from NW Mexico

Contact Info

Product

Resources

About