Distinguishing between ante factum and post factum properties of animal cell lines and demonstrating their use in grouping ray-finned fish cell lines into invitromes

Bols, Niels C.; Lee, Lucy E. J.; Dowd, Georgina C.

doi:10.1007/s11626-022-00744-0

Cited by 12 publications

(3 citation statements)

References 168 publications

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“…The totality of these cell lines constitutes the fish invitrome (Bols et al, 2017). The fish invitrome can be subdivided into smaller invitromes, based on different themes, such as species or organ of origin (Bols et al, 2017; Bols et al, 2023). For example, cell lines from Oplegnathus punctatus constitute the spotted knifejaw invitrome and cell lines from the brains of fish are the fish brain invitrome.…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of a brain tissue cell line from spotted knifejaw (Oplegnathus punctatus) and its susceptibility to several fish viruses

Liao

Zhang

Kang

et al. 2023

Journal of Fish Diseases

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Cells are important in the study of virus isolation and identification, viral pathogenic mechanisms and antiviral immunity. The spotted knifejaw (Oplegnathus punctatus) is a significant farmed fish in China that has been greatly affected by diseases in recent years. In this study, a new cell line derived from the spotted knifejaw brain (SKB) was established and characterized. SKB cells multiplied well in Leibovitz's L‐15 medium supplemented with 10% fetal bovine serum at 28°C. Chromosome analysis revealed that modal chromosome number was 48 for SKB. SKB cells exhibit susceptibility to several fish viruses, such as a largemouth bass virus, red grouper nervous necrosis virus (RGNNV), infectious spleen and kidney necrosis virus (ISKNV), Singapore grouper iridovirus (SGIV) and spotted knifejaw iridovirus isolate (SKIV‐TJ), as shown by cytopathic effect and increased viral titers. Electron microscopy results showed that the cytoplasm contained a large number of vacuoles, and many virus particles existed at the edge of the vacuoles in RGNNV‐infected cells and numerous viral particles were scattered throughout the cytoplasm in both ISKNV‐ and SKIV‐TJ‐infected cells. These results suggest that SKB is an ideal tool for studying host–virus interactions and potential vaccine development.

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Section: Introductionmentioning

confidence: 99%

Establishment and characterization of a brain tissue cell line from spotted knifejaw (Oplegnathus punctatus) and its susceptibility to several fish viruses

Liao

Zhang

Kang

et al. 2023

Journal of Fish Diseases

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“…Fish cell lines has been established as an essential tool for investigating many key issues in aquaculture, such as disease, reproduction, genetics, biotechnology and environmental toxicology (Bols et al, 2005, 2023; Goswami et al, 2022). Since the establishment of the first fish cell line, RTG‐ 2 in 1962, more than 783 fish cell lines have been established from various fish tissues including gill, fin, eye, liver, and kidney (Lakra et al, 2011; Wolf & Quimby, 1962).…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of a liver cell line from silver pomfret (Pampus argenteus) for studying fish health

Jiang

Zhang

Wang

et al. 2023

Journal of Fish Diseases

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Fish cell lines have become a useful tool to study in resource conservation, genetic breeding, diseases control, and environmental pollutants detection. The silver pomfret (Pampus argenteus) is a high‐valued marine fish species in aquaculture, which is seriously threatened by various fish diseases. In this study, a new cell line derived from P. argenteus liver (PaL) was established and characterized. PaL cells mainly consisted of fibroblast‐like morphology and multiplied well in Leibovitz's L‐15 medium supplemented with 15% foetal bovine serum and 3 ng/mL basic fibroblast growth factor at 22°C. Amplification of the Cyt b gene confirmed that the origin of PaL cells as P. argenteus. Chromosome analysis revealed that PaL cells had a diploid Karyotyp. The PaL cells were efficiently transfected with pEGFP‐N3 plasmids, indicating its potential application in foreign gene manipulation studies. The PaL cells were found to be susceptible to red sea bream iridovirus (RSIV) and the expression of immune‐related gene (TLR5) and apoptosis‐related genes (Bax, Cyt c3, CASP9) were upregulated. Furthermore, lipopolysaccharide and palmitic acid (PA) treatments decreased cell viability and up‐regulated the expression of inflammation related genes (IL‐8, IL‐1β). Meanwhile, PA incubation induced cell apoptosis by Bcl‐2‐regulated caspase activation. In conclusion, the newly established PaL cell line will be an appropriate in vitro tool for viral propagation and immune response.

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“…Structurally speaking, most of the muscles of L. crocea belong to the white muscle of vertebrates, which is mainly composed of skeletal muscle and fat [3]. Fish skeletal muscle cell lines are often described simply by their predominate cell shape, which usually is described as either fibroblast-like, epithelial-like or spindle-like [4]. All of these cell lines can contribute to studies on in vitro meat production, and both their ante factum properties, Fishes 2023, 8, 429 2 of 14 such as the species and anatomical site from which they arose, and post factum properties, such as growth factor requirements, are important features to consider [5].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Proliferation Medium and Its Effect on Differentiation of Muscle Satellite Cells from Larimichthys crocea in Cultured Fish Meat Production

Zhang,

Lou,

et al. 2023

Fishes

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To find a suitable medium for muscle satellite cells of Larimichthys crocea, herein, the effect of different basal media and coating materials on the proliferation of piscine satellite cells (PSCs) was explored. Firstly, two basal media, namely F10 and DMEM/F12, were selected as experimental materials, and high-sugar DMEM was the main basal culture medium used with fish muscle cells as a control. The results showed that the PSCs proliferated better in F10 than in DMEM/F12 or DMEM. Secondly, the effects of rat tail collagen, polylysine and matrix coatings, as compared with no coating, on the proliferation and later differentiation of PSCs were also investigated. Our results indicated that there was no significant difference between coating and no coating on the proliferation of PSCs in the F10-based medium. Meanwhile, it was found that the myotubes were washed out, and only those under matrix-coated conditions remained intact in the process of differentiation. The results also suggested that PSCs could still differentiate into myotubes without their stemness being affected after proliferation in the F10-based medium. Hence, this study identified an efficient proliferation medium based on F10 basal medium that could shorten the culture time and maintain the stemness of PSCs, thus providing a basis for large-scale cell expansion and cell-culture-based meat production in the future.

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Distinguishing between ante factum and post factum properties of animal cell lines and demonstrating their use in grouping ray-finned fish cell lines into invitromes

Cited by 12 publications

References 168 publications

Establishment and characterization of a brain tissue cell line from spotted knifejaw (Oplegnathus punctatus) and its susceptibility to several fish viruses

Establishment and characterization of a brain tissue cell line from spotted knifejaw (Oplegnathus punctatus) and its susceptibility to several fish viruses

Establishment and characterization of a liver cell line from silver pomfret (Pampus argenteus) for studying fish health

Characterization of Proliferation Medium and Its Effect on Differentiation of Muscle Satellite Cells from Larimichthys crocea in Cultured Fish Meat Production

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