Distinguishing responses of acetoclastic and hydrogenotrophic methanogens to ammonia stress in mesophilic mixed cultures

Wang, Zhongzhong; Wang, Shun; Hu, Yuansheng; Du, Bang; Meng, Jizhong; Wu, Guangxue; Li, He; Zhan, Xinmin

doi:10.1016/j.watres.2022.119029

Cited by 36 publications

(13 citation statements)

References 48 publications

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“…Similarly, the relative abundance of Methanosaeta was also not obviously affected by the ammonia nitrogen concentration of 1.5 g N/L ( Ace 0 , 39%; Ace 1.5 , 36%). As it is well known, compared with hydrogenotrophic methanogens, acetoclastic methanogens were more susceptible to ammonia toxicity; although Methanosaeta was a methanogenic archaeon that consumes acetic acid, in this study, it was found that its abundance was less affected by the ammonia concentration of 1.5 g N/L. Conventionally speaking, the toxicity of high-concentration ammonia (≥3 g/L) would affect the growth of methanogenic archaea.…”

Section: Resultsmentioning

confidence: 57%

“…Methanobacteriales as hydrogenotrophic methanogens, decreased from 49 to 38 and 44% in the Control and Ammonia groups, respectively. Hydrogenotrophic methanogens have been found to be more tolerant to ammonia nitrogen than acetoclastic methanogens in many studies. , Further study on the population changes of archaeal communities at the genus level. Twelve methanogenic archaea were identified at the genus level (Figure d).…”

Section: Resultsmentioning

confidence: 99%

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Ammonia Stress on Anaerobic Digestion: The More Sensitive Propionic/Butyric Acid-Degrading Bacteria over Methanogens

Feng,

Lian,

Zou

et al. 2023

ACS EST Eng.

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Nitrogenous organic matter could be converted into ammonia nitrogen during anaerobic digestion (AD) which could lead to the inhibition of methane production. Therefore, it is crucial to investigate the effect of ammonia stress on AD. In this study, the addition of ammonia nitrogen concentration of 1.5 g N/ L led to an obvious extended lag-period time of the AD with propionic acid or n-butyric acid as the sole substrate, while it showed no effect on the AD of acetic acid, indicating that the biotransformation of propionic acid and butyric acid might be more susceptible under low inhibitory ammonia stress. Analysis of the microbial consortia revealed that the community composition of bacteria and archaea was not obviously affected by a low inhibitory ammonia stress. Metatranscriptomic analysis showed that the enzymes related to the biotransformation of propionic and butyric acids were more susceptible to ammonia toxicity than those corresponding to acetoclastic methanogenesis. During protein synthesis, the expression of RNA polymerase and the subunits of ribosomes, which affected the transcription process, were significantly down-regulated. Additionally, ammonia toxicity caused DNA damage in microorganisms and triggered the SOS response of microorganisms. This study provided new insights for further understanding of the mechanism of ammonia stress at a low inhibitory level.

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Section: Resultsmentioning

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Ammonia Stress on Anaerobic Digestion: The More Sensitive Propionic/Butyric Acid-Degrading Bacteria over Methanogens

Feng,

Lian,

Zou

et al. 2023

ACS EST Eng.

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“…This agrees with previous studies showing that hydrogenotrophic methanogen was likely to become the dominant species with the exposure of multiple inhibitors or stresses such as BES, 5 chloroform, 35 and ammonia. 36 The higher tolerance of hydrogenotrophic methanogen as compared with the acetoclastic one might be attributed to the following reasons. First, compared with the rod cell morphology of hydrogenotrophic methanogen, 37 the thin filaments' cell morphology of the acetoclastic one and its large surface area might lead to a faster spread of inhibitors/ stresses into bacteria, and hence, higher sensitivity.…”

Section: Unca Reduced Atp Level and Suppressed Electron Transport Bymentioning

confidence: 99%

Effect of Undissociated n-Caproic Acid on Methanogen Activity and Subsequent Recovery: Methane Anabolism and Community Structure

Li,

Qiu,

et al. 2024

ACS EST Eng.

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The anaerobically fermented medium chain carboxylic acid (MCCA) has been demonstrated to inhibit methanogenesis in an anaerobic chain elongation fermentation system. However, the inhibition mechanism and recovery performance remain unknown. This study systematically investigated the shortterm effects of undissociated n-caproic acid (UnCA) on methanogenesis in granular sludge, focusing on methane anabolism and community structure. Results showed that 2.0 mM UnCA inhibited CH 4 yield by 94.3% and acetate consumption by 88.8%, which was ascribed to the variations of intracellular H + and cell membrane damage. Specifically, UnCA altered multiple processes of methane anabolism such as energy metabolism and electron transport by increasing the intracellular H + level. In addition, the hydrophobicity of UnCA destroyed the integrity of the cell membrane. However, the methane synthesis enzymes appeared not to be affected as indicated by the stable abundance and activities and also the subsequent reversible 66.6−82.8% recovery of the CH 4 yield. Meanwhile, the UnCA supplementation transformed the dominant species from acetoclastic methanogenic to hydrogenotrophic ones and also enriched Bathyarchaeia which had potential CH 4 production capacity. These findings are expected to enhance the understanding of the MCCA inhibition on methanogenesis, promoting the establishment of an efficient and stable MCCA production system.

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“…AMs are generally predominant over HMs in AD due to their higher affinity to substrates. , However, HMs could outcompete AMs in some conditions. HMs are known to have a higher tolerance to acetate with 11 times higher ammonia tolerance to AMs. , These advantages over AMs make HMs play a more important role in the AD system for stabilized operation.…”

Section: Introductionmentioning

confidence: 99%

“…HMs are known to have a higher tolerance to acetate with 11 times higher ammonia tolerance to AMs. 6,7 These advantages over AMs make HMs play a more important role in the AD system for stabilized operation.…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Role of Conductive Materials in the Interaction between Syntrophic Bacteria and Hydrogenotrophic Methanogens: From Kinetics to Microbial Pathways

et al. 2023

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Conductive materials have been reported to enhance anaerobic digestion (AD) processes, but their role in the synergistic relationship between syntrophic bacteria and hydrogenotrophic methanogens (HMs) remains unclear yet. This study evaluated the holistic impact of conductive materials in an HMsdominant anaerobic system (HMs over 95% in the archaeal community). Biochar (BC) and iron powder (IP) were selected as conductive materials with food waste and waste-activated sludge as co-substrates. The addition of IP and BC to HMs-dominant reactors significantly increased the methane yield (11.5−26%) and shortened the digestion period (32−45%) compared with the control. In addition, a novel developed integrated kinetic model revealed the diauxic peak for methanogenesis which could be significantly alleviated by the mediation of conductive materials. Furthermore, the degradation of volatile fatty acids followed a sequential order of acetate−butyrate−propionate. A smoother degradation was observed with the addition of conductive materials, while the control groups exhibited a 10 day lag phase between the degradation of butyrate and propionate. The microbial community analysis showed that BC and IP stimulated diverse syntrophic bacteria, that is, BC-enriched genus Longilinea and Aminicenantales and IP-accumulated Syntrophomonadaceae. Moreover, IP significantly facilitated HMs enrichment. Overall, this study offers new insights into the regulation of conductive materials on microbial pathways of syntrophic and hydrogenotrophic methanogenesis, with potential implications for the development of sustainable and efficient organic waste treatment strategies.

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Distinguishing responses of acetoclastic and hydrogenotrophic methanogens to ammonia stress in mesophilic mixed cultures

Cited by 36 publications

References 48 publications

Ammonia Stress on Anaerobic Digestion: The More Sensitive Propionic/Butyric Acid-Degrading Bacteria over Methanogens

Ammonia Stress on Anaerobic Digestion: The More Sensitive Propionic/Butyric Acid-Degrading Bacteria over Methanogens

Effect of Undissociated n-Caproic Acid on Methanogen Activity and Subsequent Recovery: Methane Anabolism and Community Structure

Deciphering the Role of Conductive Materials in the Interaction between Syntrophic Bacteria and Hydrogenotrophic Methanogens: From Kinetics to Microbial Pathways

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