Distortion of micronuclei and other peripheral erythrocytes caused by fenitrothion and their recovery assemblage in zebrafish

Khatun, Mt. Marufa; Mostakim, Golam Mohammod; Moniruzzaman, M.; Rahman, Umme Ohida; Islam, Mohammad Nazrul

doi:10.1016/j.toxrep.2021.02.019

Cited by 26 publications

(21 citation statements)

References 36 publications

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“…An advantage of testing pesticides in the freshwater ecosystem is that this type of research may provide information on the recovery of the system after pesticide contamination has stopped. A 28-day recuperation period demonstrated the foremost astounding recuperation reaction, and also the decreasing micronuclei arrangement could indicate repair of damaged DNA, a great degree of intensely injured cells, or both [ 32 , 33 ]. This reverse connection between the time of application and DNA harm could be attributable to the danger of xenobiotics that disturb the enzymatic process in the formation of DNA damage [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Malathion-Induced Hematoxicity and Its Recovery Pattern in Barbonymus gonionotus

Mrong

Islam

Kole

et al. 2021

Journal of Toxicology

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An experiment was conducted to assess malathion-induced hematological responses of Barbonymus gonionotus (silver barb) and its recovery patterns in malathion-free water. Fish (45 days old) were exposed to two sublethal concentrations, namely, 25% and 50% (i.e., 3.78 and 7.56 ppm) of LC50 (15.13 ppm) of malathion for 28 days, followed by a postexposure recovery period for the same time. The hematological parameters were examined after 1, 7, 14, 21, and 28 days of exposure as well as after the postexposure recovery time. Except in the case of the control group (0% of malathion), the obtained results revealed that malathion exposure resulted in significantly ( p < 0.05 ) higher prevalence and severity of micronucleus and lower values of Hb, PCV, and RBC and significantly higher values of WBC in a concentration- and time-dependent manner. The values of blood glucose, MCV, MCH, and MCHC showed mixed trends during the experiment. During the recovery period, all blood parameters (micronucleus, glucose, Hb, PCV, RBC, WBC, MCV, MCH, and MCHC) partially recovered, which means that the recovery period was not long enough for the organisms to recover from the previous exposure. The study thus confirms that hematology is a sensitive indicator for fish to detect toxicity caused by different chemicals. Changes in these parameters can provide useful information about environmental conditions and risk assessment of aquatic organisms.

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Section: Discussionmentioning

confidence: 99%

Malathion-Induced Hematoxicity and Its Recovery Pattern in Barbonymus gonionotus

Mrong

Islam

Kole

et al. 2021

Journal of Toxicology

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“…The toxic effects induced by various pollutants can induce physiological stress leading to induction of different genotoxic abnormalities and development of morphological nuclear changes in erythrocytes of exposed aquatic animals [ 17 , 24 ]. Studies have recorded that investigation of morphological features of red blood cells are frequently utilized in toxicity testing and in determination of health condition of fish [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…Blood is commonly regarded as an effective stress indicator for revealing the potential harmful stress and health status of fish [17][18][19]. It is recorded that the monitoring and determination of hematological profile of fish is of vital, useful, and reliable tool for the estimation of different pathological ailments induced by various environmental pollutants both in aquatic and terrestrial animals.…”

Section: Introductionmentioning

confidence: 99%

Nuclear and Morphological Alterations in Erythrocytes, Antioxidant Enzymes, and Genetic Disparities Induced by Brackish Water in Mrigal Carp (Cirrhinus mrigala)

Raza

Ghaffar

Hussain

et al. 2022

Oxidative Medicine and Cellular Longevity

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Salinization of aquatic ecosystem, abrupt climate change, and anthropogenic activities cause adverse impact on agricultural land/soil as well as the aquaculture industry. This experimental study was designed to evaluate different biomarkers of oxidative stress, antioxidant enzymes, and genotoxic potential of diverse salinities of brackish water on freshwater fish. A total of 84 fresh water mrigal carp (Cirrhinus mrigala) were randomly segregated and maintained in four groups (T0, T1, T2, and T3) in a glass aquarium under similar laboratory conditions at various salinity levels (0, 3, 5, and 7 parts per thousand) to determine the pathological influence of brackish water. All the fish in groups T1, T2, and T3 were exposed to various salinity levels of brackish water for a period of 90 days while the fish of group T0 served as the control group. The experimental fish reared in different groups T1, T2, and T3 displayed various physical and behavioral ailments. The results revealed significantly augmented quantity of different oxidative stress indicators including reactive oxygen species (ROS) and thiobarbituric acid reactive substance (TBARS) in different visceral tissues (kidneys, liver, and gills) of exposed fish. Different antioxidant enzymes such as reduced glutathione (GSH), peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) along with total proteins were remarkably reduced in the kidneys, gills, and liver tissues. Results showed significantly increased values of different nuclear abnormalities (erythrocyte with micronucleus, erythrocyte with condensed nucleus, and erythrocyte with lobed nucleus) and morphological changes (pear shaped erythrocyte, spindle-shaped erythrocytes, and spherocyte) in red blood cells of experimental fish. The results on genotoxic effects exhibited significantly increased DNA damage in isolated cells of liver, kidneys, and gills of exposed fish. The findings of our experimental research suggested that brackish water causes adverse toxicological impacts on different visceral tissues of fresh water fish at higher salinity level through disruption and disorder of physiological and biochemical markers.

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“…To date the analysis of tailed and dumbbell-shaped nuclei in human biomonitoring, particularly for individuals living at high background radiation area (HBRA), is very rarely conducted. This contrasts with the fact that tailed nuclei are observed not only in human lymphocytes but also in erythrocytes of embryonic fish exposed to radiation in vivo (Braham et al 2017, Khetsuriani et al 2019, Khatun et al 2021. For this reason, the "erythrocyte micronucleus cytome assay" (ECMNA) has been used in aquatic cytogenotoxicity (Anbumani & Mohankumar 2011).…”

Section: Tailed and Dumbbell-shaped Nuclei Analysis In Human Biomonitoringmentioning

confidence: 99%

Could scoring tailed and dumbbell-shaped nuclei increase the sensitivity of micronucleus analysis as a biomarker of radiation exposure?

Ramadhani

Wulansari

Suvifan

et al. 2021

Berkala Penelitian Hayati

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In the cytokinesis-block micronucleus (CBMN) assay, micronuclei (MNi), nucleoplasmic bridges (NPBs), and nuclear budding (NBUD) are the most commonly analysed morphological types of nuclear abnormalities. In contrast, tailed and dumbbell-shaped nucleus have historically received little attention in the CBMN assay. Interestingly, the incidence of tailed and dumbbell-shaped nuclei in lymphocytes is closely related with that of dicentric chromosomes or NPBs in the CBMN assay. To provide a better picture of the implications and significance of tailed and dumbbell-shaped nuclei as markers of radiation exposure, a literature review was performed in this study. Twenty articles were found in PubMed, PubMed Central, and manually searched. The articles were screened and those that met the inclusion criteria and did not meet the exclusion criteria were reviewed by all authors. At the end, nine articles were included. In conclusion, the assessment of in vivo tailed nuclei in blood smears and accounting for the occurrence of dumbbell-shaped nuclei in the CBMN assay can increase the sensitivity of the CBMN assay for biodosimetry involving a high dose exposure.

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Distortion of micronuclei and other peripheral erythrocytes caused by fenitrothion and their recovery assemblage in zebrafish

Cited by 26 publications

References 36 publications

Malathion-Induced Hematoxicity and Its Recovery Pattern in Barbonymus gonionotus

Malathion-Induced Hematoxicity and Its Recovery Pattern in Barbonymus gonionotus

Nuclear and Morphological Alterations in Erythrocytes, Antioxidant Enzymes, and Genetic Disparities Induced by Brackish Water in Mrigal Carp (Cirrhinus mrigala)

Could scoring tailed and dumbbell-shaped nuclei increase the sensitivity of micronucleus analysis as a biomarker of radiation exposure?

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