The abundance and distribution of ichthyoplankton and their relationships to current velocity, temperature, dissolved oxygen, pH and electrical conductivity of the water in the Anavilhanas Ecological Station, Negro River, Amazonas State, Brazil, were analyzed. Preferred microhabitats for spawning, dispersion and nursery were also verified. Sampling was undertaken during the falling water period of 2001 and the rising water period of 2002, in a section of 100 km subdivided into 5 subsections, with a total of 20 stations (5 beaches, 5 ravines, 5 channels, and 5 lake channels) at night and during the day at the surface and at the bottom. 647 eggs and 4,187 larvae were captured, belonging to 10 families and four orders: Characiformes (6), Siluriformes (2), Perciformes (1), and Clupeiformes (1). Engraulidae (55.39%), Pimelodidae (30.45%), Auchenipteridae (5.23%) and Sciaenidae (5.13%) were the dominant families. The hierarchical statistical model (ANOVA) with three factors (microhabitat, depth and period) was applied to the environmental variables and the larval abundance, showing greater abundances of sciaenids in the ravines and lower abundances of engraulids in the channels. The highest captures were obtained at lower temperature values, at the bottom during the day and at the surface at night, suggesting an active larval behavior. The presence of the four larval development stages in all subsection for pimelodids and sciaenids, and in three subsections for engraulids, indicates that the Anavilhanas Ecological Station is an important spawning and nursery area for species of these groups in the Negro River. Larvae abundance of all characiform families was extremely low (from 0.1 to 1.17%), suggesting that they do not spawn in this system.A abundância e distribuição do ictioplâncton e suas relações com a velocidade da corrente, temperatura, oxigênio dissolvido, pH e condutividade elétrica da água na Estação Ecológica Anavilhanas, rio Negro, Amazonas, Brasil, foram investigadas, bem como o uso de áreas e microhabitats preferenciais para desova, dispersão e criação. As coletas foram realizadas em dois perío-dos: vazante de 2001 e enchente de 2002, em um trecho de 100 km dividido em cinco subseções, e em cada um delas foram amostrados os microhabitats praia, barranco, canal e canal de lago, com um total de 20 pontos de coletas (cinco praias, cinco barrancos, cinco canais e cinco canais de lago). As coletas foram realizadas durante o dia e à noite na superfície e no fundo. 647 ovos e 4.187 larvas de peixes foram capturados, pertencentes a quatro ordens e dez famílias: Characiformes (6), Siluriformes (2), Perciformes (1) e Clupeiformes (1). As famílias dominantes foram Engraulidae (55,39%), Pimelodidae (30,45%), Auchenipteridae (5,23%) e Sciaenidae (5,13%). O modelo de estatística hierárquica (ANOVA) com três fatores (microhabitat, profundidade e período) foi aplicado às variáveis ambientais e às abundâncias de larvas, mostrando maior abundância de Sciaenidae nos barrancos e menor de Engraulidae nos canais. As maiores ca...