Multiscale imaging systems are widely employed for high-resolution imaging purposes. However, research in this area often lacks comprehensive theoretical analysis of these systems and discussions regarding potential mechanical interference among adjacent optical paths, leading to directional inadequacies and complexities in optical design. A novel method for designing multiscale imaging systems, to our knowledge, is proposed to address this gap. This method effectively guides optical design processes and mitigates mechanical interference. First, a theoretical analysis of the field of view distribution in multiscale imaging systems is conducted alongside the proposition of a method for calculating the field of view size for individual imaging systems. Second, telecentric optical paths are introduced, establishing an optical model and deriving a calculation formula for the system’s critical aperture, thereby identifying conditions to prevent mechanical interference. Third, a telecentric multiscale imaging system with satisfactory imaging performance is designed, featuring a focal length of
−200mm
and a telecentricity of less than 0.1 mrad. The system achieves diffraction-limited performance in the diffuse spot’s radius and modulation transfer function (MTF) curve without encountering mechanical interference. This design methodology presents a novel approach for subsequent multiscale imaging system designs, offering theoretical insights and practical implications.