Distribution and Abundance of &lt;i&gt;Pulsatilla Patens&lt;/i&gt; Populations in Nature Reserves in North-Eastern Poland

Juśkiewicz-Swaczyna, Barbara

doi:10.2478/v10020-010-0035-5

Cited by 4 publications

(7 citation statements)

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“…The absence of distinctive differences between the analyzed populations can be attributed to the fact that until recently, Eastern pasqueflower had continuous geographic range in Eastern and Central Europe (Hultén & Fries, 1986; Akeroyd, 1993). Nowadays the populations of the studied species are gradually disappearing and becoming partially isolated (Ciosek, 1999; Wójtowicz, 2000; Wójtowicz, 2001; Chmura, 2003; Wójtowicz, 2004; Juśkiewicz-Swaczyna, 2010). However, this process has not been taking place long enough for evolutionary mechanisms to induce significant differences between populations.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effectiveness of ISJ and SSR markers and detection of outlier loci in conservation genetics ofPulsatilla patenspopulations

Bilska

Szczecińska

2016

PeerJ

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BackgroundResearch into the protection of rare and endangered plant species involves genetic analyses to determine their genetic variation and genetic structure. Various categories of genetic markers are used for this purpose. Microsatellites, also known as simple sequence repeats (SSR), are the most popular category of markers in population genetics research. In most cases, microsatellites account for a large part of the noncoding DNA and exert a neutral effect on the genome. Neutrality is a desirable feature in evaluations of genetic differences between populations, but it does not support analyses of a population’s ability to adapt to a given environment or its evolutionary potential. Despite the numerous advantages of microsatellites, non-neutral markers may supply important information in conservation genetics research. They are used to evaluate adaptation to specific environmental conditions and a population’s adaptive potential. The aim of this study was to compare the level of genetic variation in Pulsatilla patens populations revealed by neutral SSR markers and putatively adaptive ISJ markers (intron-exon splice junction).MethodsThe experiment was conducted on 14 Polish populations of P. patens and three P. patens populations from the nearby region of Vitebsk in Belarus. A total of 345 individuals were examined. Analyses were performed with the use of eight SSR primers specific to P. patens and three ISJ primers.ResultsSSR markers revealed a higher level of genetic variation than ISJ markers (He = 0.609, He = 0.145, respectively). An analysis of molecular variance (AMOVA) revealed that, the overall genetic diversity between the analyzed populations defined by parameters FST and ΦPT for SSR (20%) and ΦPT for ISJ (21%) markers was similar. Analysis conducted in the Structure program divided analyzed populations into two groups (SSR loci) and three groups (ISJ markers). Mantel test revealed correlations between the geographic distance and genetic diversity of Polish populations of P. patens for ISJ markers, but not for SSR markers.ConclusionsThe results of the present study suggest that ISJ markers can complement the analyses based on SSRs. However, neutral and adaptive markers should not be alternatively applied. Neutral microsatellite markers cannot depict the full range of genetic variation in a population because they do not enable to analyze functional variation. Although ISJ markers are less polymorphic, they can contribute to the reliability of analyses based on SSRs.

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Section: Discussionmentioning

confidence: 99%

Comparison of the effectiveness of ISJ and SSR markers and detection of outlier loci in conservation genetics ofPulsatilla patenspopulations

Bilska

Szczecińska

2016

PeerJ

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“…Similar relations have been reported by other authors based on many years of field studies. At the non‐forest sites, the number of individuals was greater, including a greater number of blooming and fruit‐bearing ones and a greater number of flowers on single individuals (Juśkiewicz‐Swaczyna & Grzybowski, 2013). Wójtowicz (2000) claims that with the deterioration of sunlight accessibility (increased shadow or in years with a small number of sunny days), not only the number of P. patens flowers but also the number of leaf rosettes on individuals decreases.…”

Section: Discussionmentioning

confidence: 99%

“…All tools used in modeling (diagnostic testing, cross‐validation, sensitivity analysis) are built‐in functionalities of the GeNIe software. Prior to the preliminary modeling, the discretization (the process of transforming continuous variables into contiguous intervals) was carried out using expert opinion or the literature as guidance (Table 1; Juśkiewicz‐Swaczyna & Choszcz, 2012; Juśkiewicz‐Swaczyna & Grzybowski, 2013; Zarzycki et al, 2002). The structure of the BN was learned from the data set at the significance level α = 0.05 using the Bayesian Search Algorithm:

P (()|, A_{i}, B) goodbreak= \frac{P ((), A_{i}) P (()|, B, A_{i})}{\sum_{j = 1}^{\infty} P ((), A_{j}) P (()|, B, A_{j})},

where

P (()|, A_{i}, B)

, the conditional probability (a posteriori) of event A (in this study current characteristics of P. patens populations) on condition that event B (in this study biotic and abiotic environmental factors) has occurred;

P ((), A_{i})

, the probability a priori of event A ;

\sum_{j = 1}^{\infty} P ((), A_{j}) P (()|, B, A_{j})

, the sum of probabilities of event A 1 , A 2 , …, A n (Drużdżel, 2005).…”

Section: Methodsmentioning

confidence: 99%

Bayesian network model informs on the impact of biotic and abiotic factors on the population characteristics of an endangered plant species

Sienkiewicz

Łaska

2022

Population Ecology

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Preservation of the endangered plant species requires knowledge of the impact of environmental factors on their populations. This study was undertaken to evaluate the potential of a Bayesian network (BN) model to analyze the impact of habitat conditions on the current characteristics of Pulsatilla patens (L.) Mill. population in NE Poland. The model was based on field data collected in 2011-2015 from 47 sites in the four largest forest complexes of NE Poland. The causal network between (1) the biotic and (2) abiotic factors as well as morphological-developmental features of individuals and demographic features of populations (20 attributes, 1573 records) was made using the BayesianSearch Algorithm in GeNIe 2.0. The results indicate that the greatest impact on the population features had the number of competing species in forest undergrowth and exposition of slopes. Diagnostic testing and sensitivity analysis revealed that these factors were the major variables determining the size of the population, developmental phase, and the size of individuals. Tenfold cross-validation revealed that this model was the most effective to analyze the impact of habitat conditions on the presence of generative shoots, developmental phase, and juvenile shoots. The optimal levels of factors following from the BN modeling of the impact of habitat conditions on the characteristics of the population will allow regular growth (size of individuals) and development (life cycle of individuals) of a given endangered species.

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“…The pasque-flower is included in Annex I to the Bern In Poland, P. patens is a differential species of Peucedano-Pinetum pine forests (Matuszkiewicz 2006). Less frequently, it can be found at edges of Serratulo-Pinetum, Querco roboris-Pineum mixed pine forests or Potentillo albae-Quercetum steppe oak forests (Juśkiewicz-Swaczyna 2010a, 2010bŁaska & Sienkiewicz 2010;Pawlikowski 2011). It grows in sheltered places with southern or south-western exposition (Ciosek 1999;Zych 2007), on acidic sandy soils of low nitrogen, phosphorus, magnesium and potassium content (Wójtowicz 2000).…”

Section: Introductionmentioning

confidence: 97%

Population dynamics of Pulsatilla patens (L.) Mill. in a new locality in Poland

Ciosek¹,

Piórek²,

Sikorski³

et al. 2016

Biodiversity Research and Conservation

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A new locality of Pulsatilla patens (L.) Mill. in the Wyszków-Jegiel Forest District, in the southern part of Puszcza Biała (ATPOL EC79) is described. This locality is situated on an escarpment, at the edge of Peucedano-Pinetum fresh pine forest with elements of Serratulo-Pinetum mixed pine forest and Potentillo albae-Quercetum steppe oak forest. Good light conditions prevail in the place of plant occurrence. Studies carried out in 2008-2015 showed an increasing trend of P. patens population, though, recently, this population seems to be stable. The number of species accompanying the pasque-flower also increased in this time period. It was found based on the analysis of flora directly neighbouring the clumps of the pasque-flower. During 7 years, the number of taxa increased by 31.5% on plot I and by 25% on plot II. In the years 2011-2013, the pasqueflower was represented both by flowering (1-2) and vegetative individuals. Afterwards, the plant was present only in vegetative stage. The number of its leaves varied in time. The highest number of leaves -14 per individual -was noted in 2014, and the lowest number (1) -in 2012. The reason for a decline in the number of plant clumps in the site Dalekie and, at the same time, the greatest threat was the excavation of sand from the escarpment. In 2012, this escarpment slid down and several clumps of the pasque-flower were destroyed. Forest management and digging up by wild boars were additional factors limiting the population. To preserve the site of P. patens, measures of active protection should be applied.

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Distribution and Abundance of <i>Pulsatilla Patens</i> Populations in Nature Reserves in North-Eastern Poland

Cited by 4 publications

References 5 publications

Comparison of the effectiveness of ISJ and SSR markers and detection of outlier loci in conservation genetics ofPulsatilla patenspopulations

Comparison of the effectiveness of ISJ and SSR markers and detection of outlier loci in conservation genetics ofPulsatilla patenspopulations

Bayesian network model informs on the impact of biotic and abiotic factors on the population characteristics of an endangered plant species

Population dynamics of Pulsatilla patens (L.) Mill. in a new locality in Poland

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