Distribution and antibiotic-resistance of different <i>Staphylococcus</i> species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Garbacz, Katarzyna; Wierzbowska, Maria; Kwapisz, Ewa; Kosecka-Strojek, Maja; Bronk, Marek; Saki, Morteza; Międzobrodzki, Jacek

doi:10.1080/20002297.2021.1983322

Cited by 18 publications

(15 citation statements)

References 33 publications

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“…Our Polish Reference Center for Staphylococci is a leading center for bacteriophage typing of S. aureus strains involved in various infections, with considerable achievements in this field [ 12 , 13 , 14 , 15 , 16 ]. Recently, the oral cavity has gained attention as a reservoir of antibiotic-resistant S. aureus strains and a source of their spread to other anatomical regions [ 17 , 18 , 19 ]. However, the knowledge of the sensitivity of oral S. aureus to bacteriophages and the potential therapeutic application of the latter are fairly limited.…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance

et al. 2021

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Nowadays, research on bacteriophage therapy and its potential use in combination with antibiotics has been gaining momentum. One hundred and ten oral Staphylococcus aureus isolates were phage-typed and their antibiotic resistance was determined by standard and molecular methods. The prevalence of MSSA and MRSA strains was 89.1% and 10.9%, respectively. Nearly all (91.8%) analyzed isolates, whether MSSA or MRSA, were susceptible to the phages used from the international set. The highest lytic activity showed phages 79 and 52 A from lytic group I. The predominant phage groups were mixed, the I+III group and a mixed group containing phages from at least three various lytic groups. S. aureus strains sensitive to phage group I were usually resistant to penicillin and susceptible to ciprofloxacin, whereas the strains typeable with group V or group V with the 95 phage were susceptible to most antibiotics. Epidemic CA-MRSA strains (SCCmecIV) of phage type 80/81 carried Panton–Valentine leucocidin genes. Considering the high sensitivity of oral S. aureus to the analyzed phages and the promising results of phage therapies reported by other authors, phage cocktails or phage-antibiotic combinations may potentially find applications in both the prevention and eradication of staphylococcal infections.

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Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance

et al. 2021

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“…However, the inappropriate use of antimicrobials in oral cavity is still performed in dentistry ( Holmes et al, 2016 ; Gross et al, 2021 ). Recent studies showed that the oral cavity is considered one of major reservoirs of antimicrobial resistance genes in human body ( Kearney et al, 2020 ; Garbacz et al, 2021 ). In the present study we showed that HEF and PA have a narrow-spectrum therapeutic effect against P. gingivalis , which is recognized as a keystone pathobiont for development of periodontal disease.…”

Section: Discussionmentioning

confidence: 99%

Dual Inhibitory Activity of Petroselinic Acid Enriched in Fennel Against Porphyromonas gingivalis

2022

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Increasing evidence has shown that a major periodontal pathobiont, Porphyromonas gingivalis, triggers oral dysbiosis leading to deterioration not only of periodontal health, but also of several systemic conditions. In the present study we identified remarkable anti-P. gingivalis activity of Foeniculum vulgare (fennel), an herbal plant used in Asian cuisine as well as in traditional medicine, by screening of 92 extracts prepared from 23 edible plants. The n-hexane-extracted fennel (HEF) showed a rapid lethal action toward P. gingivalis, while it was rather ineffective with a wide range of other oral commensal bacterial species. Morphological analysis using both high-speed atomic force microscopy and field emission scanning electron microscopy revealed that a low concentration of HEF (8 μg/mL) resulted in formation of protruding nanostructures composed of outer membrane vesicle (OMV)-like particles, while a high concentration of HEF (64 μg/mL) induced bacteriolysis with overproduction of OMVs with unusual surface properties. Interestingly, HEF treatment resulted in deprivation of two outer membrane transporter proteins, RagA and RagB, which is essential for nutrient acquisition in P. gingivalis, by extracellularly releasing RagA/RagB-enriched OMVs. Furthermore, HEF showed gingipain-inhibitory activity toward both arginine-specific (Rgps) and lysine-specific (Kgp) gingipains, resulting in blocking oral epithelial cell rounding and the subsequent detachment from culture dishes. Finally, we isolated petroselinic acid as a major bactericide as well as a gingipain inhibitor through a bioassay-guided fractionation of HEF. Taken together, our findings suggest clinical applicability of HEF and petroselinic acid for periodontitis therapy to eliminate P. gingivalis and its major virulence factors on the basis of the dual anti-P. gingivalis activity, i.e., rapid bacteriolysis and gingipain inhibition.

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“…Four previous studies had identified antibiotic-resistant bacteria in dental offices: S. maltophilia resistant to trimethoprim were found in an air sample of a dental waiting room [ 10 ], and S. aureus resistant to methicillin were found on the surfaces of dental consulting rooms [ 4 , 8 , 9 ]. Several Staphylococcus species resistant to antibiotics (mainly resistant to penicillin, gentamicin and erythromycin) were isolated from the oral cavity: mainly S. aureus , but also numerous coagulase-negative staphylococci including S. haemolyticus [ 25 ]. Therefore, the oral cavity should be considered to be a potential reservoir and source of antibiotic-resistant bacteria spread in the environment.…”

Section: Discussionmentioning

confidence: 99%

Microbiological Contamination of the Office Environment in Dental and Medical Practice

et al. 2021

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The microbiological contamination of the environment in independent healthcare facilities such as dental and general practitioner offices was poorly studied. The aims of this study were to describe qualitatively and quantitatively the bacterial and fungal contamination in these healthcare facilities and to analyze the antibiotic resistance of bacterial pathogens identified. Microbiological samples were taken from the surfaces of waiting, consulting, and sterilization rooms and from the air of waiting room of ten dental and general practitioner offices. Six surface samples were collected in each sampled room using agar contact plates and swabs. Indoor air samples were collected in waiting rooms using a single-stage impactor. Bacteria and fungi were cultured, then counted and identified. Antibiograms were performed to test the antibiotic susceptibility of bacterial pathogens. On the surfaces, median concentrations of bacteria and fungi were 126 (range: 0–1280) and 26 (range: 0–188) CFU/100 cm2, respectively. In indoor air, those concentrations were 403 (range: 118–732) and 327 (range: 32–806) CFU/m3, respectively. The main micro-organisms identified were Gram-positive cocci and filamentous fungi, including six ubiquitous genera: Micrococcus, Staphylococcus, Cladosporium, Penicillium, Aspergillus, and Alternaria. Some antibiotic-resistant bacteria were identified in general practitioner offices (penicillin- and erythromycin-resistant Staphylococcus aureus), but none in dental offices. The dental and general practitioner offices present a poor microbiological contamination with rare pathogenic micro-organisms.

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Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Cited by 18 publications

References 33 publications

Staphylococcus aureus Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance

Staphylococcus aureus Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance

Dual Inhibitory Activity of Petroselinic Acid Enriched in Fennel Against Porphyromonas gingivalis

Microbiological Contamination of the Office Environment in Dental and Medical Practice

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