Distribution and chemical characterization of regular arrays in the cell walls of strains of the genus Lactobacillus

Masuda, Kuniyoshi; Kawata, Tomio

doi:10.1111/j.1574-6968.1983.tb00105.x

Cited by 68 publications

(52 citation statements)

References 15 publications

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“…The disappearance of the outer layer from the isolated cell walls after SDS treatment and the concomitant loss of all protein material showed that the predominant 52 kDa protein was located in this layer and that it was in some way noncovalently associated with the underlying layer. Such a 50 kDa protein had previously been encountered in another L. helveticus strain and negative staining experiments had suggested the possibility of a regular surface arrangement (Masuda & Kawata, 1983). In our case no regular arrangement was detected by negative staining (data not shown).…”

Section: Discussionmentioning

confidence: 43%

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Cell wall and autolytic system of Lactobacillus helveticus ATCC 12046

Lortal¹,

Rousseau

Boyaval³

et al. 1991

Journal of General Microbiology

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The cell wall of Lactobacillus helveticus was found to have a three-layered structure when thin sections of whole cells or isolated cell walls were stained by various procedures and examined by electron microscopy. The main feature of the composition of isolated cell walls was a high protein content (about 48% of the cell wall dry weight) with a predominant 52 kDa protein. Peptidoglycan and what was considered as teichoic acids accounted for 36% and 14%, respectively, of the cell wall dry weight. The 52 kDa protein was apparently unglycosylated, located in the outer layer and noncovalently bound to the inner layers. Peptidoglycan was mostly assigned to the innermost layer. The conditions leading to autolysis of whole cells or isolated cell walls were determined, as well as the specificity of the autolysin involved in cell wall degradation. IntroductionLactobacillus helveticus is an important lactic acid bacterium that is involved in cheese technology (Accolas et al., 1980;Turner et al., 1983) and that has recently become the object of interest for genetic manipulation (Chassy, 1987;Sandine, 1987;Thomas & Collins, 1988). Its autolysis with the concomitant release of cytoplasmic proteases (Ezzat et al., 1986) is an essential feature of cheese maturation (Bie & Sjostrom, 1975a, b). A better understanding of the autolytic system of L. helveticus could perhaps offer a new way for an accelerated ripening of cheese. Ohmiya & Sat0 (1970) were the first to follow the autolysis of L. helveticus in technological conditions. After adding whole cells to an aseptic rennet curd, the release of DNA was noted and autolysed cells were clearly observed by electron microscopy. More recently, we promoted the autolysis of L. helveticus by suspending whole cells in dilute buffers (Lortal et al., 1989). A clear understanding of the autolytic system of L. helveticus implies, in particular, a better knowledge of the composition and structure of its cell wall, which have so far been investigated only to a limited extent (Ikawa & Snell, 1960;Baddiley & Davison, 1961 ; Williams, 1971). The main peptidoglycan amino acids were determined (Glu, Ala, Asp, Lys) as well as their respective percentage in the D-configuration (Ikawa & Snell, 1960). Previous studies also revealed the presence of glyceroltype teichoic acids (Baddiley & Davison, 1961) and neutral sugars (glucose, galactose but no rhamnose). Wall and membrane teichoic acids of L. helveticus were shown to have antigenic properties (Knox & Wicken, 1971). It has also been suggested that the cell wall of L. helveticus ATCC 10797 was composed of two layers with the outer layer presumably containing a predominant protein (Masuda & Kawata, 1983).In the work described here, a more detailed investigation of the morphology and composition of the cell wall of L. helveticus ATCC 12046 was undertaken. Moreover, the main characteristics of the autolytic system of this strain were investigated, in particular ways of promoting lysis, the effect of external factors, and the specificity of the autolytic...

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Section: Discussionmentioning

confidence: 43%

“…Wall and membrane teichoic acids of L. helveticus were shown to have antigenic properties (Knox & Wicken, 1971). It has also been suggested that the cell wall of L. helveticus ATCC 10797 was composed of two layers with the outer layer presumably containing a predominant protein (Masuda & Kawata, 1983). …”

mentioning

confidence: 99%

Cell wall and autolytic system of Lactobacillus helveticus ATCC 12046

Lortal¹,

Rousseau

Boyaval³

et al. 1991

Journal of General Microbiology

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“…In many species of lactic acid bacteria, including those of the genus Lactobacillus, the presence of surface-layer proteins [30,31,32] has been found. The gene for the S-layer protein has been sequenced and cloned in Lactobacillus brevis [33], Lactobacillus acidophilus [34], Lactobacillus helveticus [35] and Lactobacillus crispatus [36].…”

Section: Microorganisms With Probiotic Propertiesmentioning

confidence: 99%

Development of New Products: Probiotics and Probiotic Foods

Denkova¹,

Krastanov²

2012

Probiotics

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“…The S protein is capable of crystallization into a regular structure on the outside of bacteria, the S-layer, which covers the entire cell wall during all stages of growth (for reviews see Beveridge, 1994; Messner & Sleytr, 1992). The S-layer is present on bacteria of several Lactobacilh~ species known to inhabit the gastro-intestinal tract (Johnson et al, 1987; Lortal, 1993;Masuda & Kawata, 1983). Schneitz et a/.…”

Section: Boot and O T H E R Smentioning

confidence: 99%

The presence of two S-layer-protein-encoding genes is conserved among species related to Lactobacillus acidophilus

et al. 1996

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BCCM/LMGPreviously w e have shown that the type strain of Lactobacillus acidophilus possesses two S-protein-encoding genes, one of which is silent, on a chromosomal segment of 6 kb. The 5-protein-encoding gene in the expression site can be exchanged for the silent S-protein-encoding gene by inversion of this slp segment. In this study the presence of S-protein and corresponding Sprotein-encoding genes of strains belonging to species that are closely related to L acidophilus was determined. All strains investigated were identified by numerical comparison of highly standardized one-dimensional SDS-PAGE whole-cellular-protein patterns. Western blot and Southern blot methods were used to identify the presence of, and homology between, S-proteins and Sprotein-encoding genes. From these analyses w e conclude that strains of L.

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Distribution and chemical characterization of regular arrays in the cell walls of strains of the genus Lactobacillus

Cited by 68 publications

References 15 publications

Cell wall and autolytic system of Lactobacillus helveticus ATCC 12046

Cell wall and autolytic system of Lactobacillus helveticus ATCC 12046

Development of New Products: Probiotics and Probiotic Foods

The presence of two S-layer-protein-encoding genes is conserved among species related to Lactobacillus acidophilus

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